07-579 | Anti-REST Antibody

07-579
200 µg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-579
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-REST Antibody
      Alternate Names
      • Neural-restrictive silencer factor
      • RE1-silencing transcription factor
      • X2 box repressor
      • neuron restrictive silencer factor
      • repressor binding to the X2 box
      Background InformationNRSF (neuron-restrictive silencer factor, also designated XBR and REST for RE1-silencing factor) is a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). The binding of NRSF to the NRSE represses neuronal gene transcription in non-neuronal cells. Although NRSF is most highly expressed in non-neural tissues, it is also expressed in developing neurons and at low levels in the brain. NRSF contains nine zinc finger domains, but also exists as a C-terminally truncated form produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and weakly binds DNA, yet is transported to the nucleus. NRSF associates with mSin3 and HDAC in ventricular myocytes, suggesting a role for NRSF outside the nervous system. Down-regulation of NRSF, which normally occurs upon neural differentiation, is necessary for the proper development of certain classes of neurons. NRSF is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract
        Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
      PresentationPurified rabbit IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Store at -20°C.
      Applications
      ApplicationAnti-REST Antibody is a rabbit polyclonal antibody for detection of REST also known as RE1-silencing transcription factor, X2 box repressor, neuron restrictive silencer factor & has been validated in WB.
      Key Applications
      • Western Blotting
      Application NotesUpdated information-Rest is glycosylated. From http://amsdottorato.cib.unibo.it/687/
      From the summary:
      "Finally, the glycosylation pattern of the REST protein was analysed, moving from the observation that the molecular weight calculated on REST sequence is about 116 kDa but using western blotting this transcription factor appears to have distinct apparent molecular weight (see Table 1.1): this difference could be explained by post-translational modifications of the proteins, like glycosylation. In fact recently, several studies underlined the importance of O-glycosylation in modulating transcriptional silencing, protein phosphorylation, protein degradation by proteasome and protein–protein interactions [Julenius et al., 2005; Zachara and Hart, 2006]. Deglycosilating analysis showed that REST protein in SH-SY5Y and HEK293 cells is Oglycosylated and not N-glycosylated. Moreover, using several combination of deglycosilating enzymes it is possible to hypothesize the presence of Gal-β(1-3)-GalNAc residues on the endogenous REST, while β(1-4)-linked galactose residues may be present on recombinant REST protein expressed in HEK293 cells. However, the O-glycosylation process produces an immense multiplicity of chemical structures and monosaccharides must be sequentially hydrolyzed by a series of exoglycosidase. Further experiments are needed to characterize all the post-translational modification of the transcription factor REST." In particular the authors show that REST migrates at 200kd in SH-SY5Y cells.
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 801-1097 of human REST (repressor element RE-1 silencing transcription factor). Also known as NRSF (neuronal restricted silencing factor).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes REST, Mr 200 kDa
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described; however, their full length nature has not been determined.
      Gene Symbol
      • REST
      • NRSF
      • XBR
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13127 # Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
      SIZE: 1097 amino acids; 121888 Da
      SUBUNIT: Interacts with SIN3A, SIN3B and RCOR1.
      SUBCELLULAR LOCATION: Nucleus (Probable).
      TISSUE SPECIFICITY: Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
      SIMILARITY: SwissProt: Q13127 ## Contains 9 C2H2-type zinc fingers.
      Molecular Weight120-200 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot with HeLa and Raji RIPA lysate.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected REST in nuclear extracts from HeLa and Raji cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Analysenzertifikate

      TitelChargennummer
      Anti-REST 2484184
      Anti-REST (rabbit polyclonal IgG) - DAM1457527 DAM1457527
      Anti-REST - 2424772 2424772
      Anti-REST - 2462854 2462854
      Anti-REST - 0701049896 0701049896
      Anti-REST - 0702052409 0702052409
      Anti-REST - 2000855 2000855
      Anti-REST - 2019731 2019731
      Anti-REST - 2087934 2087934
      Anti-REST - 2189955 2189955

      Literatur

      ÜbersichtAnwendungSpeziesPub Med ID
      Transcriptional Mechanisms of Proneural Factors and REST in Regulating Neuronal Reprogramming of Astrocytes.
      Masserdotti, G; Gillotin, S; Sutor, B; Drechsel, D; Irmler, M; Jørgensen, HF; Sass, S; Theis, FJ; Beckers, J; Berninger, B; Guillemot, F; Götz, M
      Cell stem cell  17  74-88  2015

      Abstract anzeigen
      26119235 26119235
      A comparison of the rest complex binding patterns in embryonic stem cells and epiblast stem cells.
      Seki, M; Masaki, H; Arauchi, T; Nakauchi, H; Sugano, S; Suzuki, Y
      PloS one  9  e95374  2014

      Abstract anzeigen
      24752154 24752154
      In vitro ischemia triggers a transcriptional response to down-regulate synaptic proteins in hippocampal neurons.
      Fernandes, J; Vieira, M; Carreto, L; Santos, MA; Duarte, CB; Carvalho, AL; Santos, AE
      PloS one  9  e99958  2014

      Abstract anzeigen
      Western Blotting24960035 24960035
      REST and stress resistance in ageing and Alzheimer's disease.
      Lu, T; Aron, L; Zullo, J; Pan, Y; Kim, H; Chen, Y; Yang, TH; Kim, HM; Drake, D; Liu, XS; Bennett, DA; Colaiácovo, MP; Yankner, BA
      Nature  507  448-54  2014

      Abstract anzeigen
      Immunofluorescence24670762 24670762
      Consuming a Western diet for two weeks suppresses fetal genes in mouse hearts.
      Medford, HM; Cox, EJ; Miller, LE; Marsh, SA
      American journal of physiology. Regulatory, integrative and comparative physiology  306  R519-26  2014

      Abstract anzeigen
      24523346 24523346
      RASSF1A inactivation unleashes a tumor suppressor/oncogene cascade with context-dependent consequences on cell cycle progression.
      Ram, RR; Mendiratta, S; Bodemann, BO; Torres, MJ; Eskiocak, U; White, MA
      Molecular and cellular biology  34  2350-8  2014

      Abstract anzeigen
      Western Blotting24732797 24732797
      Comparison of REST cistromes across human cell types reveals common and context-specific functions.
      Rockowitz, S; Lien, WH; Pedrosa, E; Wei, G; Lin, M; Zhao, K; Lachman, HM; Fuchs, E; Zheng, D
      PLoS computational biology  10  e1003671  2014

      Abstract anzeigen
      24922058 24922058
      REST mediates androgen receptor actions on gene repression and predicts early recurrence of prostate cancer.
      Svensson, C; Ceder, J; Iglesias-Gato, D; Chuan, YC; Pang, ST; Bjartell, A; Martinez, RM; Bott, L; Helczynski, L; Ulmert, D; Wang, Y; Niu, Y; Collins, C; Flores-Morales, A
      Nucleic acids research  42  999-1015  2014

      Abstract anzeigen
      24163104 24163104
      Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits.
      Xue, Yuanchao, et al.
      Cell, 152: 82-96 (2013)  2013

      Abstract anzeigen
      23313552 23313552
      Specificity protein 1 (Sp1)-dependent activation of the synapsin I gene (SYN1) is modulated by RE1-silencing transcription factor (REST) and 5'-cytosine-phosphoguanine (CpG) methylation.
      Paonessa, F; Latifi, S; Scarongella, H; Cesca, F; Benfenati, F
      The Journal of biological chemistry  288  3227-39  2013

      Abstract anzeigen
      23250796 23250796

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      Included Positive Control

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies