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AB5467 | Anti-AANAT Antibody

AB5467
50 µL  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, R, Sh, F WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB5467
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-AANAT Antibody
      Alternate Names
      • Arylalkylamine N-acetyltransferase
      • SNAT
      • Serotonin Acetylase
      • EC 2.3.1.87
      References
      Product Information
      FormatSerum
      Control
      • POSITIVE CONTROL:

        Night adapted rat pineal gland. Expression is light sensitive; highest levels are obtained from animals at night.
      PresentationLiquid rabbit serum containing no preservative.
      Applications
      ApplicationAnti-AANAT Antibody is an antibody against AANAT for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot: 1:10,000-1:20,000 using ECL. Reacts with the ~23 kDa AANAT protein. Some reactivity may also be seen at ~97 kDa. Suggested blocking buffer is PBS with 10% non fat dry milk and 0.2% Tween-20. Suggested dilution buffer is PBS with 1 mg/mL BSA fraction V. Suggested incubation time is overnight at room temperature (~23°C).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide (phosphorylated) corresponding to amino acids near the N-terminous of rat AANAT conjugated to BSA. {Ganguly, S et al, 2005}.
      HostRabbit
      SpecificityRecognizes AANAT (Arylalkylamine N-acetyltransferase). The antibody specifically recognizes the phosphorylated N-terminal PKA site of AANAT (pT31) (Ganguly et al. 2005). The antibody reacts with natural and recombinant protein.
      Species Reactivity
      • Bovine
      • Human
      • Rat
      • Sheep
      • Fish
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryArylalkylamine N-acetyltransferase belongs to the superfamily of acetyltransferases. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for seasonal reproduction, modulates the function of the circadian clock in the suprachiasmatic nucleus, and influences activity and sleep. This enzyme is rapidly inactivated when animals are exposed to light at night. This protein is 80% identical to sheep and rat AA-NAT. Arylalkylamine N-acetyltransferase may contribute a multifactorial genetic diseases such as altered behavior in sleep/wake cycle.
      Gene Symbol
      • AANAT
      • SNAT
      • AA-NAT
      • EC 2.3.1.87
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16613 # Catalyzes the N-acetylation of serotonin into N- acetylserotonin.
      SIZE: 207 amino acids; 23344 Da
      SUBUNIT: Monomer (By similarity).
      TISSUE SPECIFICITY: Highly expressed in pineal gland and retina.
      SIMILARITY: SwissProt: Q16613 ## Belongs to the acetyltransferase family. AANAT subfamily. & Contains 1 N-acetyltransferase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      RABBIT ANTI-AANAT, pT31, RAT 2465109
      RABBIT ANTI-AANAT, pT31, RAT POLYCLONAL ANTIBODY - 2293680 2293680

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Placental melatonin production and melatonin receptor expression are altered in preeclampsia: new insights into the role of this hormone in pregnancy.
      Dave Lanoix,Pascale Guérin,Cathy Vaillancourt
      Journal of pineal research 53 2012

      Mostrar resumen
      22686298 22686298
      Dynamics in enzymatic protein complexes offer a novel principle for the regulation of melatonin synthesis in the human pineal gland.
      Erik Maronde,Anastasia Saade,Katrin Ackermann,Hany Goubran-Botros,Cecile Pagan,Roman Bux,Thomas Bourgeron,Faramarz Dehghani,Jörg H Stehle
      Journal of pineal research 51 2011

      Mostrar resumen
      21517958 21517958
      Melatonin synthesis: 14-3-3-dependent activation and inhibition of arylalkylamine N-acetyltransferase mediated by phosphoserine-205.
      Ganguly, Surajit, et al.
      Proc. Natl. Acad. Sci. U.S.A., 102: 1222-7 (2005) 2005

      Mostrar resumen
      15644438 15644438
      Characterization of the serotoninergic system in the C57BL/6 mouse skin
      Slominski, Andrzej, et al
      Eur J Biochem, 270:3335-44 (2003) 2003

      12899690 12899690

      Ficha técnica

      Cargo
      Anti-AANAT - Data Sheet