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07-741 | Anti-AS160 (Rab-GAP) Antibody

200 µL  
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      Replacement Information

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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Ca WB Rb Serum Polyclonal Antibody
      Catalogue Number07-741
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-AS160 (Rab-GAP) Antibody
      Alternate Names
      • Acrg embryonic lethality minimal region ortholog
      • Akt substrate of 160 kDa
      • TBC (Tre-2, BUB2, CDC16) domain-containing protein
      • TBC1 domain family, member
      Background InformationAS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2.
      Product Information
      • MDI-treated 3T3/L1 cell lysates.
      PresentationRabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide and 30% glycerol.
      ApplicationDetect AS160 (Rab-GAP) using this Anti-AS160 (Rab-GAP) Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 1178-1189 (C-PTNDKAKAGNKP) of rat Akt substrate of 160kDa (AS160) (Rab-GAP) with a N-terminal cysteine for conjugation purposes.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes AS160 (Rab-GAP), Mr 160 kDa.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Canine
      Species Reactivity NoteHuman and mouse. Predicted to cross react with rat and dog based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AS160
      • DKFZp779C0666
      • KIAA0603
      • OTTHUMP00000018505
      • OTTHUMP00000040818
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O60343 # May act as a GTPase-activating protein for Rab family protein(s).
      SIZE: 1298 amino acids; 146563 Da
      TISSUE SPECIFICITY: Expressed in heart, skeletal muscle and in immune tissues (spleen, lymph node and leukocytes).
      PTM: Phosphorylated by AKT1; insulin-induced. & Insulin-stimulated phosphorylation is required for GLUT4 translocation (By similarity).
      DISEASE: SwissProt: O60343 # May be involved in atopic dermatitis (AD).
      SIMILARITY: SwissProt: O60343 ## Contains 2 PID domains. & Contains 1 Rab-GAP TBC domain.
      MISCELLANEOUS: Subcellular redistribution from low density microsomes to the cytosol with insulin treatment (By similarity).
      Molecular WeightApprox. 160 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot in RIPA lysates from 3T3/L1 cells differentiated into adipocytes.

      Western Blot Analysis:
      A 1:1000-1:5000 dilution of this lot detected AS160 (Rab-GAP) in RIPA lysates from 3T3/L1 cells differentiated into adipocytes by MDI treatment. This lot also detects AS160 (Rab-GAP) in HEK293 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information


      Ficha datos de seguridad (MSDS)


      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-AS160 (Rab GAP) - 30554 30554
      Anti-AS160 (Rab GAP) - DAM1394820 DAM1394820
      Anti-AS160 (Rab GAP) - JBC1361857 JBC1361857
      Anti-AS160 (Rab-GAP) - 2455680 2455680
      Anti-AS160 (Rab-GAP) - 1962662 1962662
      Anti-AS160 (Rab-GAP) - 2044107 2044107
      Anti-AS160 (Rab-GAP) - 2056630 2056630
      Anti-AS160 (Rab-GAP) - 2190639 2190639
      Anti-AS160 (Rab-GAP) - 2309075 2309075
      Anti-AS160 (Rab-GAP) - 2536834 2536834
      Anti-AS160 (Rab-GAP) - DAM1604977 DAM1604977
      Anti-AS160 (Rab-GAP) - DAM1745368 DAM1745368
      Anti-AS160 (Rab-GAP) - JBC1913124 JBC1913124
      Anti-AS160 (Rab-GAP) -2664284 2664284
      Anti-AS160 (Rab-GAP) -2724296 2724296
      Anti-AS160 (Rab-GAP) -2766478 2766478

      Referencias bibliográficas | 41 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      PKB-Mediated Thr649 Phosphorylation of AS160/TBC1D4 Regulates the R-Wave Amplitude in the Heart.
      Quan, C; Xie, B; Wang, HY; Chen, S
      PloS one 10 e0124491 2015

      Mostrar resumen
      25923736 25923736
      Acute mTOR inhibition induces insulin resistance and alters substrate utilization in vivo.
      Kleinert, M; Sylow, L; Fazakerley, DJ; Krycer, JR; Thomas, KC; Oxbøll, AJ; Jordy, AB; Jensen, TE; Yang, G; Schjerling, P; Kiens, B; James, DE; Ruegg, MA; Richter, EA
      Molecular metabolism 3 630-41 2014

      Mostrar resumen
      Western Blotting25161886 25161886
      Increased postexercise insulin sensitivity is accompanied by increased AS160 phosphorylation in slow-twitch soleus muscle.
      Iwabe, M; Kawamoto, E; Koshinaka, K; Kawanaka, K
      Physiological reports 2 2014

      Mostrar resumen
      25501433 25501433
      Insulin signaling in skeletal muscle of HIV-infected patients in response to endurance and strength training.
      Broholm, C; Mathur, N; Hvid, T; Grøndahl, TS; Frøsig, C; Pedersen, BK; Lindegaard, B
      Physiological reports 1 e00060 2013

      Mostrar resumen
      24303139 24303139
      AS160 deficiency causes whole-body insulin resistance via composite effects in multiple tissues.
      Wang, HY; Ducommun, S; Quan, C; Xie, B; Li, M; Wasserman, DH; Sakamoto, K; Mackintosh, C; Chen, S
      The Biochemical journal 449 479-89 2013

      Mostrar resumen
      23078342 23078342
      6-Mercaptopurine augments glucose transport activity in skeletal muscle cells in part via a mechanism dependent upon orphan nuclear receptor NR4A3.
      Liu, Q; Zhu, X; Xu, L; Fu, Y; Garvey, WT
      American journal of physiology. Endocrinology and metabolism 305 E1081-92 2013

      Mostrar resumen
      24022864 24022864
      A persistent increase in insulin-stimulated glucose uptake by both fast-twitch and slow-twitch skeletal muscles after a single exercise session by old rats.
      Xiao, Y; Sharma, N; Arias, EB; Castorena, CM; Cartee, GD
      Age (Dordrecht, Netherlands) 35 573-82 2013

      Mostrar resumen
      Western Blotting22286902 22286902
      Specialized sorting of GLUT4 and its recruitment to the cell surface are independently regulated by distinct Rabs.
      Sadacca, LA; Bruno, J; Wen, J; Xiong, W; McGraw, TE
      Molecular biology of the cell 24 2544-57 2013

      Mostrar resumen
      Western Blotting23804653 23804653
      The Rab-GTPase-activating protein TBC1D1 regulates skeletal muscle glucose metabolism.
      Szekeres, F; Chadt, A; Tom, RZ; Deshmukh, AS; Chibalin, AV; Björnholm, M; Al-Hasani, H; Zierath, JR
      American journal of physiology. Endocrinology and metabolism 303 E524-33 2012

      Mostrar resumen
      Western Blotting22693207 22693207
      Muscle-specific knock-out of NUAK family SNF1-like kinase 1 (NUAK1) prevents high fat diet-induced glucose intolerance.
      Inazuka, F; Sugiyama, N; Tomita, M; Abe, T; Shioi, G; Esumi, H
      The Journal of biological chemistry 287 16379-89 2012

      Mostrar resumen
      Western BlottingMouse22418434 22418434