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MAB1501 | Anti-Actin Antibody, clone C4

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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A ELISA, ICC, IF, IHC, IH(P), WB M Ascites Monoclonal Antibody
      Catalogue NumberMAB1501
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Actin Antibody, clone C4
      Alternate Names
      • MAB1501X
      • MAB1501R
      Background InformationActin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific (Otey, 1986; Lessard, 1988).
      Product Information
      • HeLa whole cell lysate.
      PresentationMouse monoclonal Ascites fluid, with 0.01% sodium azide.
      ApplicationReliably and specifically detect actin using this Anti-Actin Antibody, clone C4. This highly published monoclonal antibody is validated for use in ELISA, IC, IF, IH, IH(P) & WB. This mAb is also available as a fluorescent conjugate.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesIndirect immunofluorescence at 1:100:
      Tissue culture cells -- fix with formaldehyde, treat with methanol or acetone.
      Glycerinated myofibrils -- fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts.
      Cryostat sections (6 µm) -- quick frozen in isopentane, slides treated with gelatin and formaldehyde.

      1:100-1:1,000 (Otey, 1987):On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle.

      Iodination (Lessard, 1979).

      Solid phase binding assay ELISA:
      1:800-1:1,000 dilution from a previous lot was shown to be strongly reactive with cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.

      strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified chicken gizzard actin (Lessard, 1988).
      Epitopea.a. 50-70
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityMAB1501 is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70. Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts (Lessard, 1983). Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
      Species Reactivity
      • All
      Species Reactivity NoteTo date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
      Gene Symbol
      • ACTA1
      • MPFD
      • ASMA
      • NEM1
      • CFTDM
      • ACTA
      • CFTD
      • NEM2
      • Alpha-actin-1
      • CFTD1
      • NEM3
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
      SIZE: 377 amino acids; 42051 Da
      SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
      SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
      DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
      SIMILARITY: SwissProt: P68133 ## Belongs to the actin family.
      MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
      Molecular Weight43 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected ACTIN on 10 ug of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -20°C in undiluted aliquots for up to 12 months from date of receipt. Do not store in a diluted format. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Ficha datos de seguridad (MSDS)


      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Actin, clone C4 2464499
      Anti-Actin, clone C4 -2691430 2691430
      Anti-Actin, clone C4 - 2145928 2145928
      Anti-Actin, clone C4 - 2384641 2384641
      Anti-Actin, clone C4 - 1994358 1994358
      Anti-Actin, clone C4 - 2020486 2020486
      Anti-Actin, clone C4 - 2065987 2065987
      Anti-Actin, clone C4 - 2239595 2239595
      Anti-Actin, clone C4 - 2275539 2275539
      Anti-Actin, clone C4 - 2295405 2295405
      Anti-Actin, clone C4 - JBC1780750 JBC1780750
      Anti-Actin, clone C4 - JC1604275 JC1604275
      Anti-Actin, clone C4 - JC1629852 JC1629852
      Anti-Actin, clone C4 - JC1663936 JC1663936
      Anti-Actin, clone C4 - LV1519577 LV1519577
      Anti-Actin, clone C4 - LV1547855 LV1547855
      Anti-Actin, clone C4 - LV1588663 LV1588663
      Anti-Actin, clone C4 - NG1715194 NG1715194
      Anti-Actin, clone C4 - NG1738985 NG1738985
      Anti-Actin, clone C4 - NG1812617 NG1812617
      Anti-Actin, clone C4 - NG1848416 NG1848416
      Anti-Actin, clone C4 -2483230 2483230
      Anti-Actin, clone C4 -2508783 2508783
      Anti-Actin, clone C4 -2549973 2549973
      Anti-Actin, clone C4 -2578104 2578104
      Anti-Actin, clone C4 -2604822 2604822
      Anti-Actin, clone C4 -2640549 2640549
      Anti-Actin, clone C4 -2665057 2665057
      Anti-Actin, clone C4 -2757213 2757213
      Anti-Actin, clone C4 -2795083 2795083
      Anti-Actin, clone C4 -2808225 2808225
      MOUSE ANTI-ACTIN - LV1501427 LV1501427

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