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MAB11012 | Anti-CBX-4 Antibody, clone 10H10.2

MAB11012
100 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, Mplex M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB11012
      DescriptionAnti-CBX-4 Antibody, clone 10H10.2
      Alternate Names
      • chromobox homolog 4
      Background InformationChromeobox 4 (CBX4), also known as Pc2, is a polycomb group (PcG) protein that functions as a transcriptional suppressor. CBX4 is thought to act as a co-repressor of DNMT3B, inducing transcriptional repression independent of DNA methylation. CBX4 also acts as a SUMO E3 ligase, facilitating SUMO1 conjugation by UBE2I. CBX4 is phosphorylated on Thr495 by HIPK2 upon DNA damage, which enhances its E3 SUMO-protein ligase activity.
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract or NIH3T3 whole cell lysate
      PresentationPurified mouse monoclonal IgG2bκ in PBS containing 0.05% sodium azide.
      Applications
      ApplicationUse Anti-CBX-4 Antibody, clone 10H10.2 (Mouse Monoclonal Antibody) validated in WB, Mplex to detect CBX-4 also known as chromobox homolog 4.
      Key Applications
      • Western Blotting
      • Multiplexing
      Application NotesLuminex®:
      The antibody was shown to bind CBX4, but not CBX7 or CBX8, by Luminex® assay.
      Biological Information
      ImmunogenThe immunogen was GST-tagged fusion protein containing amino acids 77-551 of murine CBX-4.
      Clone10H10.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes CBX-4.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and mouse. Does not react with rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PC2
      • NBP16
      • Pc2
      • hPC2
      • hPc2
      Non-Reactive Species
      • Rat
      Purification MethodPurified from ascites by protein G affinity chromatography.
      UniProt Number
      UniProt SummaryFunction: E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
      Pathway: Protein modification; protein sumoylation.
      Subunit structure: Interacts with histone H3-K9Me3 By similarity. Component of chromatin-associated class II PcG repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Interacts with SUV39H1 and HIPK2.
      Subcellular location: Nucleus.
      Tissue specificity: Ubiquitous.
      Post-translational modification: Phosphorylated on Thr-495 by HIPK2 upon DNA damage; which enhances E3 SUMO-protein ligase activity and promotes sumoylation on Lys-492.
      Sequence similarities: Contains 1 chromo domain.

      Molecular Weight61 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      1 μg/mL of this antibody detected CBX-4 on 20 ug of NIH3T3 cell lysate and HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-CBX-4, clone 10H10.2 - 2202477 2202477
      Anti-CBX-4, clone 10H10.2 - 2227194 2227194
      Anti-CBX-4, clone 10H10.2 - NG1576343 NG1576343
      Anti-CBX-4, clone 10H10.2 -2607884 2607884
      Anti-CBX-4, clone 10H10.2_2837183 2837183

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Polycomb protein Cbx4 promotes SUMO modification of de novo DNA methyltransferase Dnmt3a.
      Li, Bing, et al.
      Biochem. J., 405: 369-78 (2007) 2007

      17439403 17439403

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      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.