Tabla espec. clave
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|H||FC, IHC, IH(P)||M||Purified||Monoclonal Antibody|
|Description||Anti-CD57 Antibody, clone NK-1|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.|
|Material Size||100 µg|
Ficha datos de seguridad (MSDS)
|Cargo||Número de lote|
|MOUSE ANTI-HUMAN CD57 MONOCLONAL ANTIBODY - 2452193||2452193|
|MOUSE ANTI-HUMAN CD57 MONOCLONAL ANTIBODY - 2216876||2216876|
|MOUSE ANTI-HUMAN CD57 MONOCLONAL ANTIBODY -2548044||2548044|
|MOUSE ANTI-HUMAN CD57 MONOCLONAL ANTIBODY -2608150||2608150|
|Visión general referencias||Pub Med ID|
|Recognition of myelin-associated glycoprotein by the monoclonal antibody HNK-1. |
McGarry, R C, et al.
Nature, 306: 376-8 (1983) 1983
Myelin-associated glycoprotein (MAG) is a quantitatively minor component in both peripheral and central myelin sheaths that is thought to have a role in cell-cell interactions within the nervous system. We show here that a mouse monoclonal antibody, HNK-1, which is directed against human natural killer cells also recognizes an antigenic determinant of human central and peripheral nervous system white matter by immunoperoxidase staining of tissue sections. Immunoblot analysis of myelin proteins and purified extracted MAG indicates that the antigen recognized is MAG.
|Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. |
Lanier, L L, et al.
J. Immunol., 131: 1789-96 (1983) 1983
The functional and phenotypic characteristics of cells in human peripheral blood that mediate "natural killer" (NK) cytolysis have been examined with the use of multiparameter flow cytometry analysis and cell sorting. Essentially, all lymphocytes expressing NK and ADCC activity reacted with the anti-Leu-11a monoclonal antibody. The Leu-11a antigen was expressed on cytotoxic large granular lymphocytes (LGL), neutrophils, and basophils, but was not present on B cells, mitogen-activated T lymphoblasts, or Leu-1+ and Leu 4+ resting T cells. Anti-Leu-11a antibody selectively inhibited the binding of FITC heat-aggregated IgG complexes to granulocytes and LGL, and it may recognize a type of Fc receptor on these cells. Two-color FACS cell sorting indicated the existence of four lymphocyte subsets defined by the expression of Leu-11a and Leu-7 antigens. The Leu-11a+, -7- cells were highly active in 4-hr NK assays with the use of 51Cr-labeled K562 as the target. In contrast, the Leu-11a-, -7+ cells demonstrated weak activity and the Leu-11a-, -7- cells demonstrated no activity. The function of the Leu 11a+, -7+ cells varied considerably among several individuals examined. Multiparameter analysis with the use of two-color flow cytometry was used to determine the relationship between the expression of these NK-associated antigens and T and B cell-associated markers. These data indicate that considerable heterogeneity exists within human peripheral lymphocytes with regard to cell phenotype and function, but that several defined cellular subsets can be clearly revealed by using multiparameter FACS analysis and sorting.
|A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). |
Abo, T and Balch, C M
J. Immunol., 127: 1024-9 (1981) 1981
|Anti-CD57, clone NK-1 - Data Sheet|