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AB3613 | Anti-Caspase 12 Antibody, NT

AB3613
100 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB3613
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 12 Antibody, NT
      Background InformationThree distinct signaling pathways lead to apoptosis. The death receptor and mitochondrion pathways are the two mains, which involve respectively caspase-8 and caspase-9. The endoplasmic reticulum stress is the third apoptotic pathway and involves caspase-12. Localized to the ER but not to cytoplasm or mitochondria, caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including g-secretase presenilin and b-amyloid precursor protein (APP). Caspase-12 mediates ER stress-induced apoptosis as well as cytotoxicity induced by amyloid-b. Caspase-12 is ubiquitously expressed in mouse tissues.
      References
      Product Information
      FormatPurified
      PresentationAffinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
      Applications
      ApplicationThis Anti-Caspase 12 Antibody, N-terminus is validated for use in WB for the detection of Caspase 12.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:500 to 1:1000. Detects a band at ~53-49 kDa. in normal tissue extracts. Caspase 12 is a stress induced caspase localized to the ER and activated upon ER stress. Active process forms are difficult to detect in most non-transfected systems. Not all cells produce caspase 12; mouse L929 fibrosacrcoma cells are an excellent positive control line.

      Mouse brain tissue lysate can be used as a positive control as well. Caspase 12 is associated with ER membranes and not in cytoplasm or mitochondria. Caspase 12 mediates cytotoxixity induced by amyloid beta.

      Immunohistochemistry: 10μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPeptide corresponding to aa 2-17 of mouse caspase-12.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAntibody recognizes caspase 12 pro-protein in mouse extracts of normal and apoptotic cells. Caspase 12 is predicted to have a large >120aa long prodomain region. Thus this antibody recognizes only the proforms of the enzyme and not the active subunits. Uncleaved (proform) caspase 12 is prominently detected in many tissues. Active forms are difficult to detect expect in transfect cell systems. {Kalai, M et al (2003) 162:457-467}.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene SummaryCaspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene, CASP12, is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis.
      Gene Symbol
      • CASP12
      • Caspase-12.
      • CASP12P1
      UniProt Number
      UniProt SummarySIZE: 341 amino acids; 38907 Da
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 4°C for up to 6 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      RABBIT ANTI-CASPASE 12 -2599126 2599126
      RABBIT ANTI-CASPASE 12 -2762358 2762358
      RABBIT ANTI-CASPASE 12 POLYCLONAL ANTIBODY - 2187342 2187342

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      The endoplasmic reticulum stress inhibitor salubrinal inhibits the activation of autophagy and neuroprotection induced by brain ischemic preconditioning.
      Gao, B; Zhang, XY; Han, R; Zhang, TT; Chen, C; Qin, ZH; Sheng, R
      Acta pharmacologica Sinica 34 657-66 2013

      Mostrar resumen
      23603983 23603983
      Autophagy regulates endoplasmic reticulum stress in ischemic preconditioning.
      Rui Sheng,Xiao-Qian Liu,Li-Sha Zhang,Bo Gao,Rong Han,Ying-Qiu Wu,Xiang-Yang Zhang,Zheng-Hong Qin
      Autophagy 8 2012

      Mostrar resumen
      22361585 22361585
      Caspases find a new place to hide.
      Mehmet, H
      Nature, 403: 29-30 (2000) 2000

      10638735 10638735
      Characterization of seven murine caspase family members.
      Van de Craen, M, et al.
      FEBS Lett., 403: 61-9 (1997) 1997

      Mostrar resumen
      9038361 9038361
      Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors.
      Nakagawa, T, et al.
      J. Neurosurg., 81: 69-77 (1994) 1994

      Mostrar resumen
      8207529 8207529

      Ficha técnica

      Cargo
      Anti-Caspase 12, N-terminus - Data Sheet