A part of Merck

07-1466 | Anti-Cdc42 Antibody

07-1466
100 µg  
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      Descripción

      Replacement Information
      Replacement Information07-1466 is a recommended replacement for AB4201

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-1466
      ReplacesAB4201
      DescriptionAnti-Cdc42 Antibody
      Alternate Names
      • G25K
      • GTP-binding Protein
      Background Informationcdc42 is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal Actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of cdc42 stimulates filopodia formation, whereas Rac results in the formation of lamellipodia, and Rho stimulates formation of actin stress fibers. Effectors of the cdc42/Rac pathway include NADPH Oxidase (Phox) and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of cdc42.
      References
      Product Information
      FormatPurified
      Control
      • Rat Brain Lysate
      PresentationPurified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative.
      Applications
      ApplicationThis Anti-Cdc42 Antibody is validated for use in WB for the detection of Cdc42.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: Rat brain with previous lots of rabbit anti-Cdc42 (cat. # 07-1466) and rabbit anti-Rac1 (cat. # 07-14664).
      The molecular weights of Rac1 and Cdc42 are ~21 kDa.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 130-141 of human Cdc42
      (AKNKQKPITPET-C).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Cdc42, does not cross react with Rac1. Cdc42 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Cdc42 induces the formation of filopodia. Cdc42 can activate Rac (consequently, filopodia are intimately associated with lamellipodia.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]
      Gene Symbol
      • CDC42
      • CDC42Hs
      • G25K
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
      ENZYME REGULATION: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.
      SUBUNIT: The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, CDC42SE1, CDC42SE2, PARD6A, PARD6B and PARD6G (in a GTP-dependent manner). Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and TCGAP/SNX26. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ.
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
      ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
      Molecular Weight21 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on rat brain lysates.

      Western Blot Analysis: 1:500-1:2000 (0.5-2μg/mL) dilution of rat brain lysate with a previous lot of rabbit anti-Cdc42.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Cdc42 2475662
      Anti-Cdc42 - 2398896 2398896
      Anti-Cdc42 - 1973235 1973235
      Anti-Cdc42 - 2196115 2196115
      Anti-Cdc42 - 2299610 2299610
      Anti-Cdc42 - JBC1794850 JBC1794850
      Anti-Cdc42 - NG1609556 NG1609556
      Anti-Cdc42 - NG1835845 NG1835845
      Anti-Cdc42 -2514037 2514037
      Anti-Cdc42 -2604303 2604303
      Anti-Cdc42 -2685475 2685475
      Anti-Cdc42 -2739261 2739261

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      p16 Stimulates CDC42-dependent migration of hepatocellular carcinoma cells.
      Chen, YW; Chu, HC; Ze-Shiang Lin, ; Shiah, WJ; Chou, CP; Klimstra, DS; Lewis, BC
      PloS one 8 e69389 2013

      Mostrar resumen
      23894465 23894465