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05-815 | Anti-Cyclin D1 Antibody

05-815
100 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R IP, WB, ICC M Purified Monoclonal Antibody
      Description
      Catalogue Number05-815
      Replaces04-221
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cyclin D1 Antibody
      Background InformationProgression through G1 phase and transition from G1 to S phase of the cell division cycle is controlled by activation of a distinct series of serine/threonine kinase complexes which comprise of a cyclin regulatory subunit and a cyclin dependent kinase (cdk). D type cyclins are induced earlier than cyclin E in G1 and can form complexes with cdk2,-4,-5, and -6. Cyclin D1 is a nuclear protein during G1 phase and disappears from the nucleus in S-phase. Among the key cell cycle regulators, cyclin D1 has been implicated most strongly as a proto-oncogene in several human tumor types, including breast carcinomas.
      References
      Product Information
      FormatPurified
      Control
      • RIPA lysates from mouse 3T3/L1 cells
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-Cyclin D1 Antibody is a Mouse Monoclonal Antibody for detection of Cyclin D1 also known as G1/S-specific cyclin D1, PRAD1 oncogene & has been tested in IP, WB, ICC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenGST fusion protein cleaved with Factor Xa and gel purified, corresponding to complete mouse Cyclin D1
      HostMouse
      SpecificityCyclin D1
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteDoes not effectively recognize human Cyclin D1
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
      Gene Symbol
      • CCND1
      • D11S287E
      • U21B31
      • PRAD1
      • BCL1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P24385 # Essential for the control of the cell cycle at the G1/S (start) transition.
      SIZE: 295 amino acids; 33729 Da
      SUBUNIT: Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
      DISEASE: SwissProt: P24385 # A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle. & A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas [MIM:168461]. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer. & A chromosomal aberration involving CCND1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with the IgH locus.
      SIMILARITY: SwissProt: P24385 ## Belongs to the cyclin family. Cyclin D subfamily.
      Molecular Weight~36kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from mouse 3T3/L1 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Cyclin D1 (mouse monoclonal IgG1) - 2410234 2410234
      Anti-Cyclin D1 - 26958 26958

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Myocardin related transcription factors are required for coordinated cell cycle progression.
      Shaposhnikov, D; Kuffer, C; Storchova, Z; Posern, G
      Cell cycle (Georgetown, Tex.) 12 1762-72 2013

      Mostrar resumen
      23656782 23656782
      Overexpression of HOXB5, cyclin D1 and PCNA in congenital cystic adenomatoid malformation.
      Xiangyuan Wang,Debra J Wolgemuth,Laxmi V Baxi
      Fetal diagnosis and therapy 29 2011

      Mostrar resumen
      21228548 21228548
      Deficiency of the NR4A neuron-derived orphan receptor-1 attenuates neointima formation after vascular injury.
      Nomiyama, T; Zhao, Y; Gizard, F; Findeisen, HM; Heywood, EB; Jones, KL; Conneely, OM; Bruemmer, D
      Circulation 119 577-86 2009

      Mostrar resumen
      19153266 19153266
      Nerve Growth factor regulation of cyclin D1 in PC12 cells through a p21RAS extracellular signal-regulated kinase pathway requires cooperative interactions between Sp1 and nuclear factor-kappaB.
      Marampon, F; Casimiro, MC; Fu, M; Powell, MJ; Popov, VM; Lindsay, J; Zani, BM; Ciccarelli, C; Watanabe, G; Lee, RJ; Pestell, RG
      Molecular biology of the cell 19 2566-78 2008

      Mostrar resumen Artículo Texto completo
      18367547 18367547
      Regulation of PGC-1alpha and PGC-1alpha-responsive genes with forskolin-induced Schwann cell differentiation.
      Cowell, RM; Blake, KR; Inoue, T; Russell, JW
      Neuroscience letters 439 269-74 2008

      Mostrar resumen
      18538475 18538475
      Human cytomegalovirus-encoded chemokine receptor US28 promotes tumorigenesis.
      Maussang, D; Verzijl, D; van Walsum, M; Leurs, R; Holl, J; Pleskoff, O; Michel, D; van Dongen, GA; Smit, MJ
      Proceedings of the National Academy of Sciences of the United States of America 103 13068-73 2006

      Mostrar resumen Artículo Texto completo
      16924106 16924106
      The NR4A orphan nuclear receptor NOR1 is induced by platelet-derived growth factor and mediates vascular smooth muscle cell proliferation.
      Nomiyama, T; Nakamachi, T; Gizard, F; Heywood, EB; Jones, KL; Ohkura, N; Kawamori, R; Conneely, OM; Bruemmer, D
      The Journal of biological chemistry 281 33467-76 2006

      Mostrar resumen Artículo Texto completo
      16945922 16945922
      Identification of G1 kinase activity for cdk6, a novel cyclin D partner.
      Meyerson, M and Harlow, E
      Mol. Cell. Biol., 14: 2077-86 (1994) 1994

      Mostrar resumen
      8114739 8114739
      Functional interactions of the retinoblastoma protein with mammalian D-type cyclins.
      Ewen, M E, et al.
      Cell, 73: 487-97 (1993) 1993

      Mostrar resumen
      8343202 8343202
      A possible new member of tyrosine kinase family, human frt sequence, is highly conserved in vertebrates and located on human chromosome 13.
      Matsushime, H, et al.
      Jpn. J. Cancer Res., 78: 655-61 (1987) 1987

      Mostrar resumen
      3040650 3040650

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies