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MAB3199Z | Anti-E-Cadherin Antibody, clone 67A4, Azide Free

MAB3199Z
100 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, IF, FUNC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3199Z
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-E-Cadherin Antibody, clone 67A4, Azide Free
      Alternate Names
      • CD324
      • MAB3199
      References
      Product Information
      FormatPurified
      PresentationLiquid in 0.02M PBS with NaCl, pH=7.6, with no preservatives. Protein A purified immunoglobulin.
      Applications
      ApplicationAnti-E-Cadherin Antibody, clone 67A4, Azide Free is an antibody against E-Cadherin for use in FC & IF.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Affects Function
      Application NotesImmunoblotting

      FACS Analysis

      Functional blocking of E-cadherin

      Does not work well for immunoprecipitation

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenT-47D breast carcinoma line.
      Clone67A4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityAntibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
      Gene Symbol
      • CDH1
      • CDHE
      • E-cadherin
      • Arc-1
      • Uvomorulin
      • UVO
      • Cadherin-1
      • CD324
      • uvomorulin
      • ECAD
      • LCAM
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12830 # E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
      SIZE: 882 amino acids; 97456 Da
      SUBUNIT: Homodimer; disulfide-linked. Interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLG7.
      SUBCELLULAR LOCATION: Cell junction. Cell membrane; Single-pass type I membrane protein. Note=Colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm.
      TISSUE SPECIFICITY: Non-neural epithelial tissues.
      PTM: During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disaaaembly of adherens junctions.
      DISEASE: "SwissProt: P12830 # Defects in CDH1 are involved in dysfunction of the cell- cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. & Defects in CDH1 are a cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215]. & Defects in CDH1 are a cause of susceptibility to endometrial cancer [MIM:608089]."
      SIMILARITY: SwissProt: P12830 ## Contains 5 cadherin domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      MOUSE ANTI-HUMAN E-CADHERIN AZIDE-FREE MONOCLONAL ANTIBODY - 2383974 2383974
      MOUSE ANTI-HUMAN E-CADHERIN -2733680 2733680
      MOUSE ANTI-HUMAN E-CADHERIN AZIDE-FREE MONOCLONAL ANTIBODY - 2020864 2020864
      MOUSE ANTI-HUMAN E-CADHERIN AZIDE-FREE MONOCLONAL ANTIBODY - 2080846 2080846
      MOUSE ANTI-HUMAN E-CADHERIN AZIDE-FREE MONOCLONAL ANTIBODY - 2187369 2187369
      MOUSE ANTI-HUMAN E-CADHERIN AZIDE-FREE MONOCLONAL ANTIBODY - 2281670 2281670
      MOUSE ANTI-HUMAN E-CADHERIN AZIDE-FREE MONOCLONAL ANTIBODY -2750318 2750318
      MOUSE ANTI-HUMAN E-CADHERIN AZIDE-FREE MONOCLONAL ANTIBODY-2829128 2829128

      Referencias bibliográficas

      Visión general referenciasAplicación Pub Med ID
      Human colonic crypts in culture: segregation of immunochemical markers in normal versus adenoma-derived.
      Dame, MK; Jiang, Y; Appelman, HD; Copley, KD; McClintock, SD; Aslam, MN; Attili, D; Elmunzer, BJ; Brenner, DE; Varani, J; Turgeon, DK
      Laboratory investigation; a journal of technical methods and pathology 94 222-34 2014

      Mostrar resumen
      24365748 24365748
      The use of an in vitro 3D melanoma model to predict in vivo plasmid transfection using electroporation.
      Marrero, B; Heller, R
      Biomaterials 33 3036-46 2012

      Mostrar resumen
      22244695 22244695
      Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research.
      Stewart, CE; Torr, EE; Mohd Jamili, NH; Bosquillon, C; Sayers, I
      Journal of allergy 2012 943982 2012

      Mostrar resumen
      22287976 22287976
      Silencing of E7 oncogene restores functional E-cadherin expression in human papillomavirus 16-transformed keratinocytes.
      Caberg, Jean-Hubert D, et al.
      Carcinogenesis, 29: 1441-7 (2008) 2008

      Mostrar resumen
      18566017 18566017
      Controlled, scalable embryonic stem cell differentiation culture.
      Dang, Stephen M, et al.
      Stem Cells, 22: 275-82 (2004) 2004

      Mostrar resumen
      15153605 15153605
      E-cadherin-mediated interactions of thymic epithelial cells with CD103+ thymocytes lead to enhanced thymocyte cell proliferation.
      Kutlesa, Snjezana, et al.
      J. Cell. Sci., 115: 4505-15 (2002) 2002

      Mostrar resumen
      Immunoblotting (Western)12414996 12414996
      The adhesion molecule E-cadherin and a surface antigen recognized by the antibody 9C4 are selectively expressed on erythroid cells of defined maturational stages.
      Bühring, H J, et al.
      Leukemia, 10: 106-16 (1996) 1996

      Mostrar resumen
      8558914 8558914

      Folleto

      Cargo
      Human Stem Cell Systems

      Ficha técnica

      Cargo
      MOUSE ANTI-HUMAN E-CADHERIN AZIDE-FREE MONOCLONAL ANTIBODY

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies