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MABE69 | Anti-Emerin Antibody, clone 7D9

MABE69
100 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE69
      DescriptionAnti-Emerin Antibody, clone 7D9
      Alternate Names
      • Emery-Dreifuss muscular dystrophy
      • LEM domain containing 5
      Background InformationEmerin is a LEM domain-containing protein found in the nuclear membrane in vertebrates. The LEM domain, a conserved globular domain of approximately 40 amino acids, is present in many nuclear membrane associated proteins. Emerin is known to interact with nuclear lamins, barrier-to-autointegration factor (BAF), nesprin-1 alpha, and a transcription repressor. Functionally, this protein mediates membrane anchorage to the cytoskeleton, stabilizes and promotes formation of the nuclear actin cortical network, and inhibits beta-catenin activity by preventing nuclear accumulation. Mutations to emerin result in Dreifuss-Emery muscular dystrophy, an X-linked inherited degenerative myopathy. This protein appears to play a role in response to mechanical strain and the control of apoptosis due to strain. Emerin may also play a part in chromosome location and anchoring.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-Emerin Antibody, clone 7D9 (Mouse Monoclonal Antibody) validated in WB, ICC to detect Emerin also known as Emery-Dreifuss muscular dystrophy, LEM domain containing 5.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:50 dilution from a representative lot detected Emerin in normal human muscle cells. Courtesy of Dr. Ian Holt, Wolfson Centre for Inherited Neuromuscular Disease.
      Biological Information
      ImmunogenFull length recombinant protein corresponding to human Emerin.
      Clone7D9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryEmerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq].
      Gene Symbol
      • EDMD
      • LEMD5
      • STA
      • emerin
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.

      SUBUNIT STRUCTURE: Interacts with lamins A and C, BANF1, GMCL, BCLAF1 and YTHDC1/YT521. Interacts with TMEM43; the interaction retains emerin in the nuclear inner membrane. Interacts with SUN1 and SUN2 (By similarity). Interacts with ACTB, SPTAN1, F-actin, CTNNB1 and beta-tubulin.

      SUBCELLULAR LOCATION: Nucleus inner membrane; Single-pass membrane protein; Nucleoplasmic side. Nucleus outer membrane. Note: Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus.

      TISSUE SPECIFICTY: Skeletal muscle, heart, colon, testis, ovary and pancreas.

      PTM: Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle.

      INVOLVEMENT IN DISEASE: Defects in EMD are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1) [MIM:310300]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects.

      Sequence similarities Contains 1 LEM domain.
      Molecular Weight ~29 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Emerin on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Emerin, clone 7D9 - 2377084 2377084
      Anti-Emerin, clone 7D9 - NRG1838234 NRG1838234
      Anti-Emerin, clone 7D9 -2533848 2533848

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Direct interaction between emerin and lamin A.
      Clements, L; Manilal, S; Love, DR; Morris, GE
      Biochemical and biophysical research communications 267 709-14 2000

      Mostrar resumen
      10673356 10673356
      Distribution of emerin and lamins in the heart and implications for Emery-Dreifuss muscular dystrophy.
      Manilal, S; Sewry, CA; Pereboev, A; Man, N; Gobbi, P; Hawkes, S; Love, DR; Morris, GE
      Human molecular genetics 8 353-9 1999

      Mostrar resumen
      9949197 9949197
      The Emery-Dreifuss muscular dystrophy protein, emerin, is a nuclear membrane protein.
      Manilal, S; Nguyen, TM; Sewry, CA; Morris, GE
      Human molecular genetics 5 801-8 1996

      Mostrar resumen
      8776595 8776595