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AB1511 | Anti-Glutamate Decarboxylase 65 & 67 Antibody

AB1511
50 µL  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Fe IHC, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB1511
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glutamate Decarboxylase 65 & 67 Antibody
      Alternate Names
      • 65 kDa glutamic acid decarboxylase
      • Glutamate decarboxylase 65 kDa isoform
      • Glutamate decarboxylase-2 (pancreas)
      • glutamate decarboxylase 2
      • glutamate decarboxylase 2 (pancreatic islets and brain,65kD)
      • glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
      Background InformationGutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.
      References
      Product Information
      FormatPurified
      Control
      • Whole rat brain lysate, mouse brain cell extract.
      PresentationPurified rabbit polyclonal in buffer containing PBS-BSA containing 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Glutamate Decarboxylase 65 & 67 Antibody is validated for use in IH, WB for the detection of Glutamate Decarboxylase 65 & 67.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      1:200-1:5,000 dilution of a previous lot was used in immunohistochemistry (overnight PAP or ABC). Several fixative solutions may be used. Aldehyde-combination fixatives (i.e. those containing formaldehyde and glutaraldehyde) usually give satisfactory results. Bouin and Susa fixatives containing 0.1-0.2% glutaraldehyde have been used satisfactorily. Also reactive on paraffin embedded tissue. Protease digestion is not required.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenA synthetic peptide with the amino acid sequence [C]DFLIEEIERLGQDL from rat glutamate decarboxylase (GAD65; C-terminus residues [Cys] + 572-585) (1).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityGlutamate Decarboxylase (GAD65 and GAD67).
      Western blot reveals a doublet at approximately 65/68 kDa.
      Immunohistochemical staining can be abolished by preincubation with 1-10 μg peptide (Catalog Number AG252) per mL of diluted antibody.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Feline
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome.
      Gene Symbol
      • GAD2
      • MGC161607
      • GAD65
      • MGC161605
      • GAD-65
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q05329 # Catalyzes the production of GABA.
      COFACTOR: Pyridoxal phosphate.
      SIZE: 585 amino acids; 65411 Da
      SUBUNIT: Homodimer (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
      PTM: Phosphorylated; which does not affect kinetic parameters or subcellular location. & Palmitoylated; which is required for presynaptic clustering.
      SIMILARITY: SwissProt: Q05329 ## Belongs to the group II decarboxylase family.
      Molecular Weight65/68 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on mouse brain lysates.

      Western Blot Analysis:
      1:500 dilution of this antibody detected GAD 65&67 on 10 μg of mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots (not less than 10 μL) from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Glutamate Decarboxylase 65 67 -
      Anti-Glutamate Decarboxylase 65 67 - 2118925 2118925
      Anti-Glutamate Decarboxylase 65 67 - 2136558 2136558
      Anti-Glutamate Decarboxylase 65 67 - 1964954 1964954
      Anti-Glutamate Decarboxylase 65 67 - 2008630 2008630
      Anti-Glutamate Decarboxylase 65 67 - 2060942 2060942
      Anti-Glutamate Decarboxylase 65 67 - 2064692 2064692
      Anti-Glutamate Decarboxylase 65 67 - 2069924 2069924
      Anti-Glutamate Decarboxylase 65 67 - 2188467 2188467
      Anti-Glutamate Decarboxylase 65 67 - JBC1766713 JBC1766713
      Anti-Glutamate Decarboxylase 65 67 - JC1658245 JC1658245
      Anti-Glutamate Decarboxylase 65 67 - JC1682877 JC1682877
      Anti-Glutamate Decarboxylase 65 67 - LV1462025 LV1462025
      Anti-Glutamate Decarboxylase 65 67 - NG1723895 NG1723895
      Anti-Glutamate Decarboxylase 65 67 - NG1737444 NG1737444
      Anti-Glutamate Decarboxylase 65 67 - NG1780582 NG1780582
      Anti-Glutamate Decarboxylase 65 67 - NG1817608 NG1817608
      Anti-Glutamate Decarboxylase 65 67 - NG1938405 NG1938405
      Anti-Glutamate Decarboxylase 65 & 67 2464528
      Anti-Glutamate Decarboxylase 65 & 67 - 2360918 2360918
      Anti-Glutamate Decarboxylase 65 & 67 - 2365921 2365921
      Anti-Glutamate Decarboxylase 65 & 67 - 2390388 2390388
      Anti-Glutamate Decarboxylase 65 & 67 - 2277832 2277832
      Anti-Glutamate Decarboxylase 65 & 67 - 2324512 2324512
      Anti-Glutamate Decarboxylase 65 & 67 - 2365528 2365528
      Anti-Glutamate Decarboxylase 65 & 67 -2500412 2500412
      Anti-Glutamate Decarboxylase 65 & 67 -2580693 2580693
      Anti-Glutamate Decarboxylase 65 & 67 -2620157 2620157
      Anti-Glutamate Decarboxylase 65 & 67 -2646478 2646478
      Anti-Glutamate Decarboxylase 65 & 67 -2664971 2664971
      Anti-Glutamate Decarboxylase 65 & 67 -2718324 2718324
      Anti-Glutamate Decarboxylase 65 & 67 -2772777 2772777

      Referencias bibliográficas | 71 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      Neurovascular crosstalk between interneurons and capillaries is required for vision.
      Usui, Y; Westenskow, PD; Kurihara, T; Aguilar, E; Sakimoto, S; Paris, LP; Wittgrove, C; Feitelberg, D; Friedlander, MS; Moreno, SK; Dorrell, MI; Friedlander, M
      The Journal of clinical investigation 125 2335-46 2015

      Mostrar resumen
      25915585 25915585
      Genome-Wide Definition of Promoter and Enhancer Usage during Neural Induction of Human Embryonic Stem Cells.
      Poletti, V; Delli Carri, A; Malagoli Tagliazucchi, G; Faedo, A; Petiti, L; Mazza, EM; Peano, C; De Bellis, G; Bicciato, S; Miccio, A; Cattaneo, E; Mavilio, F
      PloS one 10 e0126590 2015

      Mostrar resumen
      25978676 25978676
      Repeated neonatal propofol administration induces sex-dependent long-term impairments on spatial and recognition memory in rats.
      Gonzales, EL; Yang, SM; Choi, CS; Mabunga, DF; Kim, HJ; Cheong, JH; Ryu, JH; Koo, BN; Shin, CY
      Biomolecules & therapeutics 23 251-60 2015

      Mostrar resumen
      25995824 25995824
      Transmitter inputs to different motoneuron subgroups in the oculomotor and trochlear nucleus in monkey.
      Zeeh, C; Mustari, MJ; Hess, BJ; Horn, AK
      Frontiers in neuroanatomy 9 95 2015

      Mostrar resumen
      26257611 26257611
      Human Induced Pluripotent Stem Cell Derived Neuronal Cells Cultured on Chemically-Defined Hydrogels for Sensitive In Vitro Detection of Botulinum Neurotoxin.
      Pellett, S; Schwartz, MP; Tepp, WH; Josephson, R; Scherf, JM; Pier, CL; Thomson, JA; Murphy, WL; Johnson, EA
      Scientific reports 5 14566 2015

      Mostrar resumen
      Immunofluorescence26411797 26411797
      microRNA-125 distinguishes developmentally generated and adult-born olfactory bulb interneurons.
      Akerblom, M; Petri, R; Sachdeva, R; Klussendorf, T; Mattsson, B; Gentner, B; Jakobsson, J
      Development (Cambridge, England) 141 1580-8 2014

      Mostrar resumen
      Immunohistochemistry24598163 24598163
      CCL2-ethanol interactions and hippocampal synaptic protein expression in a transgenic mouse model.
      Gruol, DL; Vo, K; Bray, JG; Roberts, AJ
      Frontiers in integrative neuroscience 8 29 2014

      Mostrar resumen
      Western Blotting24772072 24772072
      In utero exposure to valproic acid changes sleep in juvenile rats: a model for sleep disturbances in autism.
      Cusmano, DM; Mong, JA
      Sleep 37 1489-99 2014

      Mostrar resumen
      Western Blotting25142574 25142574
      Increased astrocyte expression of IL-6 or CCL2 in transgenic mice alters levels of hippocampal and cerebellar proteins.
      Gruol, DL; Vo, K; Bray, JG
      Frontiers in cellular neuroscience 8 234 2014

      Mostrar resumen
      25177271 25177271
      Leptin potentiates GABAergic synaptic transmission in the developing rodent hippocampus.
      Guimond, D; Diabira, D; Porcher, C; Bader, F; Ferrand, N; Zhu, M; Appleyard, SM; Wayman, GA; Gaiarsa, JL
      Frontiers in cellular neuroscience 8 235 2014

      Mostrar resumen
      25177272 25177272

      Ficha técnica

      Cargo
      Anti-Glutamate Decarboxylase 65 & 67 - Data Sheet

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies