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MAB1921 | Anti-Laminin β1 Antibody, clone 4E10

MAB1921
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ELISA, IHC, IP, WB M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB1921
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Laminin β1 Antibody, clone 4E10
      Alternate Names
      • Laminin B1 Chain
      References
      Product Information
      FormatAscites
      PresentationLiquid.
      Applications
      ApplicationAnti-Laminin β1 Antibody, clone 4E10 detects level of Laminin β1 & has been published & validated for use in ELISA, IH, IP & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesELISA (50% maximal binding to human laminin): >1:1,600,000.

      Immunofluorescence

      Affinity chromatography

      Immunoprecipitation

      Immunoblotting

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified human laminin
      Clone4E10
      HostMouse
      SpecificityReacts with the B1 portion of intact laminin and blocks the neurite stimulating activity of laminin.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryLaminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.
      Gene Symbol
      • LAMB1
      • MGC142015
      • CLM
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07942 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
      SIZE: 1786 amino acids; 198066 Da
      SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-1 is a subunit of laminin-1 (EHS laminin), laminin-2 (merosin), and laminin-6 (K-laminin).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
      DOMAIN: SwissProt: P07942 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI and IV are globular.
      SIMILARITY: Contains 13 laminin EGF-like domains. & Contains 1 laminin IV type B domain. & Contains 1 laminin N-terminal domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      MOUSE ANTI-HUMAN LAMININ MONOCLONAL ANTIBODY - 2391118 2391118
      MOUSE ANTI-HUMAN LAMININ -2517842 2517842
      MOUSE ANTI-HUMAN LAMININ -2566775 2566775
      MOUSE ANTI-HUMAN LAMININ -2610458 2610458
      MOUSE ANTI-HUMAN LAMININ -2714549 2714549
      MOUSE ANTI-HUMAN LAMININ -2736552 2736552
      MOUSE ANTI-HUMAN LAMININ MONOCLONAL ANTIBODY - 2032712 2032712
      MOUSE ANTI-HUMAN LAMININ MONOCLONAL ANTIBODY - 2075103 2075103
      MOUSE ANTI-HUMAN LAMININ MONOCLONAL ANTIBODY - 2191971 2191971
      MOUSE ANTI-HUMAN LAMININ MONOCLONAL ANTIBODY - 2322532 2322532

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      The osteogenic differentiation of human bone marrow MSCs on HUVEC-derived ECM and β-TCP scaffold.
      Kang, Y; Kim, S; Bishop, J; Khademhosseini, A; Yang, Y
      Biomaterials 33 6998-7007 2012

      Mostrar resumen
      22795852 22795852
      Laminin alpha2 chain-positive vessels and epidermal growth factor in lung neuroendocrine carcinoma: a model of a novel cooperative role of laminin-2 and epidermal growth factor in vessel neoplastic invasion and metastasis.
      Vitolo, D; Ciocci, L; Deriu, G; Spinelli, S; Cortese, S; Masuelli, L; Morrone, S; Filice, MJ; Coloni, GF; Natali, PG; Baroni, CD
      The American journal of pathology 168 991-1003 2006

      Mostrar resumen
      16507913 16507913
      A sarcoglycan-dystroglycan complex anchors Dp116 and utrophin in the peripheral nervous system.
      M Imamura, K Araishi, S Noguchi, E Ozawa
      Human molecular genetics 9 3091-100 2000

      Mostrar resumen
      11115854 11115854
      Abnormal expression of laminin beta 1 chain in skeletal muscle of adult-onset limb-girdle muscular dystrophy.
      M Li, D W Dickson, A J Spiro, M Li, D W Dickson, A J Spiro
      Archives of neurology 54 1457-61 1997

      Mostrar resumen
      9400354 9400354
      Increased laminin A expression in regenerating myofibers in neuromuscular disorders.
      Mundegar, R R, et al.
      Muscle Nerve, 18: 992-9 (1995) 1995

      Mostrar resumen
      7543975 7543975
      Appearance and distribution of laminin A chain isoforms and integrin alpha 2, alpha 3, alpha 6, beta 1, and beta 4 subunits in the developing human small intestinal mucosa.
      N Perreault, P H Vachon, J F Beaulieu, N Perreault, P H Vachon, J F Beaulieu
      The Anatomical record 242 242-50 1995

      Mostrar resumen
      7545357 7545357
      Human laminin isolated in a nearly intact, biologically active form from placenta by limited proteolysis.
      Wewer, U, et al.
      J. Biol. Chem., 258: 12654-60 (1983) 1983

      Mostrar resumen
      6415055 6415055
      Enzyme immunoassay ELISA and EMIT.
      Engvall, E
      Meth. Enzymol., 70: 419-39 (1980) 1980

      6999296 6999296

      Ficha técnica

      Cargo
      Anti-Laminin beta1, clone 4E10 - Data Sheet