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07-1799 | Anti-NCoA-2 Antibody

07-1799
100 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ChIP Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-1799
      DescriptionAnti-NCoA-2 Antibody
      Alternate Names
      • Transcriptional intermediary factor 2
      • glucocorticoid receptor-interacting protein-1
      • nuclear receptor coactivator 2
      • glucocorticoid receptor interacting protein 1
      • steroid receptor coactivator-2
      • transcriptional mediators/intermediary factor 2
      Background InformationNCoA2 (nuclear receptor coactivator 2) is a transcriptional coactivator for steroid receptors and nuclear receptors. Also known as glucocorticoid receptor interacting protein 1 (GRIP1), steroid receptor coactivator-2 (SRC-2), or transcriptional mediators/intermediary factor 2 (TIF2), this coregulatory protein contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. NCoA-2 assists nuclear receptors in the upregulation of DNA expression. In cells ligand activated nuclear receptors recruit NCoA-2 to DNA where it acylates histones to make targeted regions accessible to transcriptional machinery.

      References
      Product Information
      FormatPurified
      Control
      • U2 OS cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-NCoA-2 Antibody (rabbit polyclonal antibody) validated in WB to detect NCoA-2 also known as Transcriptional intermediary factor 2, glucocorticoid receptor-interacting protein-1, nuclear receptor coactivator 2.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      A representative lot of this antibody was used for chIP. See Monroe, D.G., et al. (2006) J. Biol. Chem. 281(39):28596-28604.
      Biological Information
      ImmunogenLinear peptide corresponding to human NCoA-2
      EpitopeUnknown
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes NCoA-2.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with human. Homology to mouse and rat is 95%.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NCOA2
      • GRIP1
      • KAT13C
      • NCoA-2
      • SRC2
      • TIF2
      • bHLHe75
      • hTIF2
      • SRC-2
      • TIF-2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.

      SUBUNIT STRUCTURE: Present in a complex containing CARM1 and EP300/P300, and interacts with CARM1 and NR3C2 By similarity. Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with HIF1A, NCOA1, APEX and NR3C1. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with ESR1, RARA and RXRA.

      SUBCELLULAR LOCATION: Nucleus.

      DOMAIN: Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant. Ref.4

      The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.

      Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.

      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.

      INVOLVEMENT IN DISEASE: Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv8(p11;q13) generates the MYST3-NCOA2 oncogene, which consists of the N-terminus part of MYST3/MOZ and the C-terminus part of NCOA2/TIF2. MYST3-NCOA2 binds to CREBBP and disrupts its function in transcription activation.

      SEQUENCE SIMILARITIES: Belongs to the SRC/p160 nuclear receptor coactivator family.

      Contains 1 basic helix-loop-helix (bHLH) domain.

      Contains 1 PAS (PER-ARNT-SIM) domain.
      Molecular Weight~ 160 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in U2 OS cell lysate.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected SRC2 on 10 µg of U2 OS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications