A part of Merck

MAB345 | Anti-O4 Antibody, clone 81

MAB345
50 µg  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Al finalizar el pedido Más información
Ahorró ()
 
Solicitar precio
Disponibilidad a confirmarDisponibilidad a confirmar
En existencia 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente

      Ofertas especiales

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Click To Print This Page

      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, M, R ICC, IHC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB345
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-O4 Antibody, clone 81
      Alternate Names
      • Sulfatide
      Background InformationOligodendrocytes and astrocytes are derived from common precursor cells, glioblasts (Sommer, 1981). O-Antigens are sulfatides, which function as differentiation markers on the surface of oligodendrocytes of the central nervous system. O4 is formed postnatally (Schachner, 1981) and is a marker for cell bodies and processes of oligodendrocytes types I and II (hairy eyeball type). During oligodendrocyte differentiation, O4 occurs in pro-oligodendrocytes, but not in O-2A-progenitor cells. O4 occurs from day 3 onwards in cell cultures of embryonic mouse brain. Anti-O4 can be used in myelination, demyelination and remyelination studies and in regeneration experiments.
      References
      Product Information
      FormatPurified
      Control
      • Rat cortical stem cells or day 3 cell cultures of brains from mouse embryos
      PresentationPurified immunoglobulin in 0.05M Potassium phosphate buffer, pH 8.0 with 0.3M NaCl and 0.05% sodium azide.
      Applications
      ApplicationAnti-O4 Antibody, clone 81 is an antibody against O4 for use in IC, IH with more than 50 product citations.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Applications Not Recommended
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry: 10-20 μg/mL on unfixed, shock frozen tissue.

      Immunocytochemistry: 10-20 μg/mL on cells fixed with 4% paraformaldehyde.

      Note: O4 is a sulfatide, which can be dissolved out of the membrane by organic solvents; acetone and methanol should not be used for fixation.

      Optimal working dilutions must be determined by the end user.

      Immunohistochemistry protocol

      1. Prepare sections from unfixed, shock frozen tissue. The sections should be 4-5 μm thick. Place the sections on microscope slides.

      2. Wash the slide three times for 5 min. each in PBS at room temperature.

      3. Block the non-specific binding sites by incubating the sections in a humid chamber with 5% FCS at room temperature for 30 minutes.

      4. Wash the slides as described in step 2.

      5. Cover the sections with a sufficient amount of MAB345 (10-20 μg/mL in PBS) and incubate in a humid chamber at 37°C for one hour.

      6. Wash the slides briefly three times with PBS. Carefully dry around the area to be stained.

      7. Cover the sections with a sufficient amount of anti-mouse IgM-fluorescein* solution and incubate in a humid chamber at 37°C for one hour.

      8. Wash the slides as described in step 6.

      9. Cover the sections with a suitable embedding medium, cover with a cover slip, and examine by fluorescence microscopy.



      *HRP or ABC can also be used.



      Optimal results can be obtained by titrating the primary and secondary antibodies



      Immunocytochemistry



      1. Fix the preparations with 4% paraformaldehyde (in PBS) at room temperature for 10 minutes. O4 is a sulfatide which can be dissolved out of the membrane by organic solvents; acetone and methanol should not be used for fixation.

      2. Wash the slide three times for 5 min. each in PBS at room temperature.

      3. Block the non-specific binding sites by incubating the sections in a human chamber with 5% FCS at room temperature for 30 minutes.

      4. Wash the slides as described in step 2.

      5. Cover the sections with a sufficient amount of MAB345 (10-20 μg/mL in PBS) and incubate in a humid chamber at 37°C for one hour.

      6. Wash the slides briefly three times with PBS. Carefully dry around the area to be stained.

      7. Cover the sections with a sufficient amount of anti-mouse IgM-fluorescein solution and incubate in a humid chamber at 37°C for one hour.

      8. Wash the slides as described in step 6.

      9. Cover the sections with a suitable embedding medium, cover with a cover slip, and examine by fluorescence microscopy.



      Note: Do not allow the preparations to dry out during staining.
      Biological Information
      ImmunogenHomogenate of white matter of corpus callosum from bovine brain.
      Clone81 (also referred to in the literature as mAB O4)
      ConcentrationVaries, see lot specific CoA
      HostMouse
      SpecificityRecognizes Oligodendrocyte marker O4. Also reacts with certain galactolipids in sperm (see Additional Information library for list).
      IsotypeIgM
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Purification MethodAmmonium Sulfate Precipitation
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-O4, clone 81 - 2138970 2138970
      Anti-O4, clone 81 - 2455696 2455696
      Anti-O4, clone 81 - 2141806 2141806
      Anti-O4, clone 81 - 2266482 2266482
      Anti-O4, clone 81 - 2289146 2289146
      Anti-O4, clone 81 -2517799 2517799
      Anti-O4, clone 81 -2571196 2571196
      Anti-O4, clone 81 -2594292 2594292
      Anti-O4, clone 81 -2691341 2691341
      Anti-O4, clone 81 -2727278 2727278
      Anti-O4, clone 81 -2779497 2779497
      Anti-p75 Low Affinity Nerve Growth -2568539 2568539
      MOUSE ANTI-OLIGODENDROCYTE MARKER O4 MONOCLONAL ANTIBODY - 1994372 1994372

      Referencias bibliográficas | 92 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
      Edri, R; Yaffe, Y; Ziller, MJ; Mutukula, N; Volkman, R; David, E; Jacob-Hirsch, J; Malcov, H; Levy, C; Rechavi, G; Gat-Viks, I; Meissner, A; Elkabetz, Y
      Nature communications 6 6500 2015

      Mostrar resumen
      25799239 25799239
      The adhesion G protein-coupled receptor GPR56 is a cell-autonomous regulator of oligodendrocyte development.
      Giera, S; Deng, Y; Luo, R; Ackerman, SD; Mogha, A; Monk, KR; Ying, Y; Jeong, SJ; Makinodan, M; Bialas, AR; Chang, BS; Stevens, B; Corfas, G; Piao, X
      Nature communications 6 6121 2015

      Mostrar resumen
      25607655 25607655
      The adhesion GPCR Gpr56 regulates oligodendrocyte development via interactions with Gα12/13 and RhoA.
      Ackerman, SD; Garcia, C; Piao, X; Gutmann, DH; Monk, KR
      Nature communications 6 6122 2015

      Mostrar resumen
      25607772 25607772
      Non-aggregating tau phosphorylation by cyclin-dependent kinase 5 contributes to motor neuron degeneration in spinal muscular atrophy.
      Miller, N; Feng, Z; Edens, BM; Yang, B; Shi, H; Sze, CC; Hong, BT; Su, SC; Cantu, JA; Topczewski, J; Crawford, TO; Ko, CP; Sumner, CJ; Ma, L; Ma, YC
      The Journal of neuroscience : the official journal of the Society for Neuroscience 35 6038-50 2015

      Mostrar resumen
      25878277 25878277
      Targeting endothelial junctional adhesion molecule-A/ EPAC/ Rap-1 axis as a novel strategy to increase stem cell engraftment in dystrophic muscles.
      Giannotta, Monica, et al.
      EMBO Mol Med, 6: 239-58 (2014) 2014

      Mostrar resumen
      24378569 24378569
      Systemic injection of neural stem/progenitor cells in mice with chronic EAE.
      Donegà, M; Giusto, E; Cossetti, C; Schaeffer, J; Pluchino, S
      Journal of visualized experiments : JoVE 2014

      Mostrar resumen
      ImmunofluorescenceMouse24798882 24798882
      Analysis of Mll1 deficiency identifies neurogenic transcriptional modules and Brn4 as a factor for direct astrocyte-to-neuron reprogramming.
      Potts, MB; Siu, JJ; Price, JD; Salinas, RD; Cho, MJ; Ramos, AD; Hahn, J; Margeta, M; Oldham, MC; Lim, DA
      Neurosurgery 75 472-82; discussion 482 2014

      Mostrar resumen
      24887289 24887289
      A phenotypic culture system for the molecular analysis of CNS myelination in the spinal cord.
      Davis, H; Gonzalez, M; Stancescu, M; Love, R; Hickman, JJ; Lambert, S
      Biomaterials 35 8840-5 2014

      Mostrar resumen
      25064806 25064806
      Pten loss in Olig2 expressing neural progenitor cells and oligodendrocytes leads to interneuron dysplasia and leukodystrophy.
      Maire, CL; Ramkissoon, S; Hayashi, M; Haidar, S; Ramkissoon, L; DiTomaso, E; Ligon, KL
      Stem cells (Dayton, Ohio) 32 313-26 2014

      Mostrar resumen
      24395742 24395742
      Generation of highly purified neural stem cells from human adipose-derived mesenchymal stem cells by Sox1 activation.
      Feng, N; Han, Q; Li, J; Wang, S; Li, H; Yao, X; Zhao, RC
      Stem cells and development 23 515-29 2014

      Mostrar resumen
      24138016 24138016

      Licencias necesarias e Información técnica

      Cargo
      Derivation of oligodendrocyte progenitor cells from a human neural stem cell line

      Ficha técnica

      Cargo
      Anti-O4, clone 81 (also referred to in the literature as mAB O4) - Data Sheet

      Newsletters / Publications

      Title
      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011

      Productos y aplicaciones relacionados

      Familias de productos

      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies