A part of Merck

AB9610 | Anti-Olig-2 Antibody

AB9610
100 µL  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Al finalizar el pedido Más información
Ahorró ()
 
Solicitar precio
Disponibilidad a confirmarDisponibilidad a confirmar
En existencia 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente

      Ofertas especiales

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Click To Print This Page

      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R ICC, IHC, IH(P), IP, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB9610
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Olig-2 Antibody
      Alternate Names
      • Oligodendrocyte transcription factor 2
      • Oligo2
      • Class B basic helix-loop-helix protein 1
      • bHLHb1
      • Class E basic helix-loop-helix protein 19
      • bHLHe19
      • Protein kinase C-binding protein 2
      • Protein kinase C-binding protein RACK17
      Background InformationOlig2 (oligodendrocyte transcription factor 2) is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. It is an antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. The restricted expression of Olig2 in oligodendrocytes makes AB9610 an excellent marker of oligodendroglia and oligodendrogliomas in development and mature systems.
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain whole cell lysate, PC12 whole cell lysate, brain (rat) tissue lysate
      PresentationPurified rabbit polyclonal. Liquid in 100 mM Glycine, 200 mM Tris, pH 8.0.
      Applications
      ApplicationAnti-Olig-2 Antibody is an antibody against Oligodendrocyte transcription factor 2 for use in IC, IH, IH(P), IP and WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blot Analysis:
      1:2,500-1:5,000 dilution of a previous lot was used in western blot on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).

      Immunoprecipitation:
      1:500-1:1,000 with human oligodendroglioma and mouse transfected cell line.
      Optimal working dilutions must be determined by the end user.

      Immunocytochemistry:
      1:500-1:1,000 dilution of a previous lot was used in immunocytochemistry on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).

      Optimal working dilutions must be determined by the end user
      Biological Information
      ImmunogenRecombinant mouse Olig-2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes the ~32 kDa Olig-2 protein by Western blot.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, rat and mouse. Other species have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome.
      Gene Symbol
      • OLIG2
      • bHLHB1
      • RACK17
      • OLIGO2
      • BHLHB1
      • PRKCBP2
      • Oligo2
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13516 # Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development (By similarity).
      SIZE: 323 amino acids; 32385 Da
      SUBUNIT: Interacts with NKX2-2 (By similarity).
      SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2 (By similarity).
      TISSUE SPECIFICITY: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
      DOMAIN:SwissProt: Q13516 The bHLH is essential for interaction with NKX2-2 (By similarity).DISEASE:SwissProt: Q13516 # A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
      SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain. ...
      Molecular Weight~32 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by immunohistochemistry on glioblastoma

      Immunohistochemistry (Paraffin):
      Representative lot data.
      Anti-Olig2 (AB9610) staining pattern morphology in glioblastoma. Tissue was pretreated with TE Buffer, pH 9.0. Polyclonal antibody was diluted to 1:500, using IHC-Select® Detection with HRP-DAB.
      Optimal Staining With TE Buffer Epitope Retrieval: Glioblastoma
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Olig-2 - 2387441 2387441
      Anti-Olig-2 - 2397064 2397064
      Anti-Olig-2 - 2436305 2436305
      Anti-Olig-2 - 2458978 2458978
      Anti-Olig-2 - 2467174 2467174
      Anti-Olig-2 - 1940104 1940104
      Anti-Olig-2 - 1994653 1994653
      Anti-Olig-2 - 2020487 2020487
      Anti-Olig-2 - 2041106 2041106
      Anti-Olig-2 - 2060464 2060464
      Anti-Olig-2 - 2089856 2089856
      Anti-Olig-2 - 2165826 2165826
      Anti-Olig-2 - 2262616 2262616
      Anti-Olig-2 - 2276294 2276294
      Anti-Olig-2 - 2325047 2325047
      Anti-Olig-2 - JBC1780805 JBC1780805
      Anti-Olig-2 - LV1538805 LV1538805
      Anti-Olig-2 - LV1583533 LV1583533
      Anti-Olig-2 - NG1722298 NG1722298
      Anti-Olig-2 - NG1752075 NG1752075
      Anti-Olig-2 - NG1848424 NG1848424
      Anti-Olig-2 -2488724 2488724
      Anti-Olig-2 -2519344 2519344
      Anti-Olig-2 -2583426 2583426
      Anti-Olig-2 -2603404 2603404
      Anti-Olig-2 -2651976 2651976
      Anti-Olig-2 -2701583 2701583
      Anti-Olig-2 -2728398 2728398
      Anti-Olig-2 -2772792 2772792
      Anti-Olig-2 -2809968 2809968

      Referencias bibliográficas | 105 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      Characterization of the neural stem cell gene regulatory network identifies OLIG2 as a multifunctional regulator of self-renewal.
      Mateo, JL; van den Berg, DL; Haeussler, M; Drechsel, D; Gaber, ZB; Castro, DS; Robson, P; Crawford, GE; Flicek, P; Ettwiller, L; Wittbrodt, J; Guillemot, F; Martynoga, B
      Genome research 25 41-56 2015

      Mostrar resumen
      25294244 25294244
      Neuronal activity regulates remyelination via glutamate signalling to oligodendrocyte progenitors.
      Gautier, HO; Evans, KA; Volbracht, K; James, R; Sitnikov, S; Lundgaard, I; James, F; Lao-Peregrin, C; Reynolds, R; Franklin, RJ; Káradóttir, RT
      Nature communications 6 8518 2015

      Mostrar resumen
      26439639 26439639
      SOX2 reprograms resident astrocytes into neural progenitors in the adult brain.
      Niu, W; Zang, T; Smith, DK; Vue, TY; Zou, Y; Bachoo, R; Johnson, JE; Zhang, CL
      Stem cell reports 4 780-94 2015

      Mostrar resumen
      25921813 25921813
      T396I mutation of mouse Sufu reduces the stability and activity of Gli3 repressor.
      Makino, S; Zhulyn, O; Mo, R; Puviindran, V; Zhang, X; Murata, T; Fukumura, R; Ishitsuka, Y; Kotaki, H; Matsumaru, D; Ishii, S; Hui, CC; Gondo, Y
      PloS one 10 e0119455 2015

      Mostrar resumen
      25760946 25760946
      Oligodendrocyte birth and death following traumatic brain injury in adult mice.
      Dent, KA; Christie, KJ; Bye, N; Basrai, HS; Turbic, A; Habgood, M; Cate, HS; Turnley, AM
      PloS one 10 e0121541 2015

      Mostrar resumen
      25798924 25798924
      GABAergic regulation of cerebellar NG2 cell development is altered in perinatal white matter injury.
      Zonouzi, M; Scafidi, J; Li, P; McEllin, B; Edwards, J; Dupree, JL; Harvey, L; Sun, D; Hübner, CA; Cull-Candy, SG; Farrant, M; Gallo, V
      Nature neuroscience 18 674-82 2015

      Mostrar resumen
      25821912 25821912
      Localization of reelin signaling pathway components in murine midbrain and striatum.
      Sharaf, A; Rahhal, B; Spittau, B; Roussa, E
      Cell and tissue research 359 393-407 2015

      Mostrar resumen
      Mouse25418135 25418135
      Subset of early radial glial progenitors that contribute to the development of callosal neurons is absent from avian brain.
      García-Moreno, F; Molnár, Z
      Proceedings of the National Academy of Sciences of the United States of America 112 E5058-67 2015

      Mostrar resumen
      26305942 26305942
      Attenuation of Cerebral Ischemic Injury in Smad1 Deficient Mice.
      Wong, JK; Chen, L; Huang, Y; Sehba, FA; Friedel, RH; Zou, H
      PloS one 10 e0136967 2015

      Mostrar resumen
      26317208 26317208
      Double minute amplification of mutant PDGF receptor α in a mouse glioma model.
      Zou, H; Feng, R; Huang, Y; Tripodi, J; Najfeld, V; Tsankova, NM; Jahanshahi, M; Olson, LE; Soriano, P; Friedel, RH
      Scientific reports 5 8468 2015

      Mostrar resumen
      25683249 25683249

      Folleto

      Cargo
      SNAP i.d. 2.0 System Brochure

      Licencias necesarias e Información técnica

      Cargo
      Derivation of oligodendrocyte progenitor cells from a human neural stem cell line

      Ficha técnica

      Cargo
      Anti-Olig2 - Data Sheet

      Newsletters / Publications

      Title
      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011

      Productos y aplicaciones relacionados

      Familias de productos

      View Gene Related Products for this Target

      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies