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612161 | Anti-Tissue Factor Mouse mAb (TF9-10H10)

612161
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      Descripción

      Replacement Information

      Tabla espec. clave

      Species ReactivityHostAntibody Type
      H, Pm M Monoclonal Antibody

      Precios y disponibilidad

      Número de referenciaDisponiblidad Embalaje Cant./Env. Precio Cantidad
      US1612161-500UG
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      Disponibilidad a confirmarDisponibilidad a confirmar
      En existencia 
      Suspendido
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      Debe confirmarse disponibilidad
        El resto: se avisará
          El resto: se avisará
          Se avisará
          Póngase en contacto con el Servicio de Atención al Cliente

          Frasco de vidrio 500 μg
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          Description
          OverviewRecognizes the tissue factor protein.
          Catalogue Number612161
          Brand Family Calbiochem®
          Application Data
          Detection of human tissue factor by immunoblotting. Sample: Whole cell lysate from JEG-3 cells. Primary antibody: Anti-Tissue Factor Mouse mAb (TF9-10H10) (Cat. No. 612161) (1:1000). Detection: chemiluminescence.
          References
          Product Information
          FormLyophilized
          FormulationLyophilized from 100 mM mannitol, 150 mM PBS, pH 7.4.
          PreservativeNone
          Applications
          Application ReferencesFlow Cytometry Veltrop, M.H., et al. 2001. Infect. Immun. 69, 2797. ELISA, Frozen Sections, Paraffin Sections, Immunocytochemistry Contrino, J., et al. 1994. Am. J. Pathol. 145, 1315. Original Clone, Immunoblotting, Dot Blot, Inhibition Studies, Neutralization Studies Morrissey, J.H., et al. 1988. Thromb. Res. 52, 247. Immunoblotting Ott, I., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 20, 874.
          Key Applications Dot Blot
          Enzyme-Linked Immunosorbent Assay
          Flow Cytometry
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Not Neutralization Studies
          Paraffin Sections
          Application NotesDot Blot (see application references)
          ELISA (see application references)
          Flow Cytometry (see application references)
          Frozen Sections (see application references)
          Immunoblotting (1:1000, see application references)
          Immunofluorescence (see application references)
          Paraffin Sections (see application references)
          Neutralization Studies (not recommended)
          Application CommentsSpecific for epitope locus I. Recognizes reduced and native, non-reduced human and primate tissue factor. This antibody does not inhibit coagulation or neutralize factor VII binding to TF. Significant reactivity with tissue factor from species other than human and primate has not been established. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpurified apoprotein of human tissue factor
          ImmunogenHuman
          Epitopewithin extracellular domain
          CloneTF9-10H10
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Primate
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute lyophilized antibody with 0.5 ml sterile dH₂O. Aliquot and freeze (-20°C) for long-term storage. For short-term storage at 4°C, add 0.02% sodium azide. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          Certificados de análisis

          CargoNúmero de lote
          612161
          Ficha técnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision24-January-2016 JSW
          ApplicationDot Blot (see application references)
          ELISA (see application references)
          Flow Cytometry (see application references)
          Frozen Sections (see application references)
          Immunoblotting (1:1000, see application references)
          Immunofluorescence (see application references)
          Paraffin Sections (see application references)
          Neutralization Studies (not recommended)
          Application Data
          Detection of human tissue factor by immunoblotting. Sample: Whole cell lysate from JEG-3 cells. Primary antibody: Anti-Tissue Factor Mouse mAb (TF9-10H10) (Cat. No. 612161) (1:1000). Detection: chemiluminescence.
          DescriptionPurified mouse monoclonal antibody (see application references). Recognizes the tissue factor protein.
          BackgroundTissue factor (TF) is the membrane receptor for coagulation factors VII and VIIa and is a cell-surface initiator of coagulation. It is the major molecule of this type and is critical for controlling hemostasis, thrombosis, and inflammation. TF is not generally present in or on cells within the vasculature, but following vascular injury, blood can come into contact with TF expressed by extravascular cells, resulting in activation of the coagulation cascade.
          HostMouse
          Immunogen speciesHuman
          Immunogenpurified apoprotein of human tissue factor
          Epitopewithin extracellular domain
          CloneTF9-10H10
          IsotypeIgG₁
          Specieshuman, primate, not rabbit
          FormLyophilized
          FormulationLyophilized from 100 mM mannitol, 150 mM PBS, pH 7.4.
          PreservativeNone
          CommentsSpecific for epitope locus I. Recognizes reduced and native, non-reduced human and primate tissue factor. This antibody does not inhibit coagulation or neutralize factor VII binding to TF. Significant reactivity with tissue factor from species other than human and primate has not been established. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute lyophilized antibody with 0.5 ml sterile dH₂O. Aliquot and freeze (-20°C) for long-term storage. For short-term storage at 4°C, add 0.02% sodium azide. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          Application referencesFlow Cytometry Veltrop, M.H., et al. 2001. Infect. Immun. 69, 2797. ELISA, Frozen Sections, Paraffin Sections, Immunocytochemistry Contrino, J., et al. 1994. Am. J. Pathol. 145, 1315. Original Clone, Immunoblotting, Dot Blot, Inhibition Studies, Neutralization Studies Morrissey, J.H., et al. 1988. Thromb. Res. 52, 247. Immunoblotting Ott, I., et al. 2000. Arterioscler. Thromb. Vasc. Biol. 20, 874.