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05-1307 | Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2, rabbit monoclonal

05-1307
100 µL  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Rb FC, ICC, IP, IHC, WB, Cell Function Assay Rb Purified Monoclonal Antibody
      Description
      Catalogue Number05-1307
      DescriptionAnti-Ubiquitin Antibody, Lys48-Specific, clone Apu2, rabbit monoclonal
      OverviewDI-UBIQUITIN PROTEIN AVAILABLE!
      Di-Ubiquitin (Lys48-linked) Recombinant Protein (17-10408)
      Background InformationPolyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.

      In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
      References
      Product Information
      FormatPurified
      Control
      • Lys48 polyubiquitin chains.
      PresentationProtein A purified recombinant purified recombinant rabbit monoclonal antibody that consists of a humanized fAb fragment fused to a rabbit Fc tail produced in a CHO recombinant antibody system. Supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide
      Applications
      ApplicationAnti-Ubiquitin Antibody, Lys48-Specific, clone Apu2 is a high quality Rabbit Monoclonal Antibody for the detection of Ubiquitin & has been validated in FC, ICC, IP, IHC, WB, PIA.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunoprecipitation
      • Immunohistochemistry
      • Western Blotting
      • Cell Function Assay
      Application NotesImmunohistochemistry:
      Ubiquitin (Lys 48-specific) staining of rat brain sections (left figure) or human breast carcinoma (right figure). Staining pattern detects axons of neurons in brain tissue but not surrounding glial cells, and breast tumor cell cytoplasm is strongly detected without nuclear involvement.

      Immunocytochemistry:
      Confocal IF analysis of NIH/3T3, HeLa, and A431 cells using anti-Ubiquitin (Lys48 specific) (Red). Actin filaments were labeled with AlexaFluor 488-Phalloidin (Green). Nuclear material is stained with DAPI (Blue).

      Flow cytometry:
      Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys48-specific (#05-1307) (blue) and rabbit IgG control (red).

      Immunoprecipitation:
      Rabbit monoclonal anti-ubiquitin (Lys-48-specific), clone Apu2, precipitated ubiquitin-containing proteins from HeLa cell lysates.

      Inhibition Assay:
      Preincubation of anti-ubiquitin, Lys48-specific, with K48-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.
      Biological Information
      ImmunogenImmunogen used was human di-ubiquitin linked at Lys48.
      EpitopeLys48 ubiquitination site
      CloneApu2
      HostRabbit
      SpecificityRecognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      Species Reactivity NoteHuman, mouse, rat, rabbit. Based on sequence homology, broad species cross-reactivity is expected.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ref.21

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.

      PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.

      MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).

      SIMILARITY: Belongs to the ubiquitin family.

      Molecular Weightvaries
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      A 1:1000-1:5000 dilution of this lot detected Lys-48 ubiquitin chains by Western blotting.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 2080891 2080891
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 2385989 2385989
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 2460206 2460206
      Anti-Ubiquitin, Lys48-Specific, -2571570 2571570
      Anti-Ubiquitin, Lys48-Specific, -2593165 2593165
      Anti-Ubiquitin, Lys48-Specific, -2652959 2652959
      Anti-Ubiquitin, Lys48-Specific, -2680524 2680524
      Anti-Ubiquitin, Lys48-Specific, -2697401 2697401
      Anti-Ubiquitin, Lys48-Specific, -2724416 2724416
      Anti-Ubiquitin, Lys48-Specific, -2762345 2762345
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 2470957
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 1983709 1983709
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 1991290 1991290
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 2017699 2017699
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 2028601 2028601
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 2036691 2036691
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 2049282 2049282
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 2145090 2145090
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 2197314 2197314
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - 2299608 2299608
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - DAM1601889 DAM1601889
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - DAM1606788 DAM1606788
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - DAM1614971 DAM1614971
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - DAM1623893 DAM1623893
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - JH1778203 JH1778203
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - NG1593364 NG1593364
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - NG1638990 NG1638990
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - NG1726951 NG1726951
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - NG1739934 NG1739934
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - NG1752251 NG1752251
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - NG1827433 NG1827433
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - NG1853764 NG1853764
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - NG1866939 NG1866939
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - NG1884914 NG1884914
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - NG1894425 NG1894425
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 - NG1940121 NG1940121
      Anti-Ubiquitin, Lys48-Specific, clone Apu2 -2500587 2500587
      Anti-Ubiquitin, Lys48-Specific, clone Apu2, Monoclonal Antibody 2840426

      Referencias bibliográficas | 31 Disponible | Ver todas las referencias

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      Differences in long-term memory stability and AmCREB level between forward and backward conditioned honeybees (Apis mellifera).
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      Stability of structured Kaposi's sarcoma-associated herpesvirus ORF57 protein is regulated by protein phosphorylation and homodimerization.
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      Journal of virology 89 3256-74 2015

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      Pharmacological targeting of valosin containing protein (VCP) induces DNA damage and selectively kills canine lymphoma cells.
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      BMC cancer 15 479 2015

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      Endocytic Adaptor Protein Tollip Inhibits Canonical Wnt Signaling.
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      PloS one 10 e0130818 2015

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      TRIM30α Is a Negative-Feedback Regulator of the Intracellular DNA and DNA Virus-Triggered Response by Targeting STING.
      Wang, Y; Lian, Q; Yang, B; Yan, S; Zhou, H; He, L; Lin, G; Lian, Z; Jiang, Z; Sun, B
      PLoS pathogens 11 e1005012 2015

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      Coupled local translation and degradation regulate growth cone collapse.
      Deglincerti, A; Liu, Y; Colak, D; Hengst, U; Xu, G; Jaffrey, SR
      Nature communications 6 6888 2015

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      Differential roles of the ubiquitin proteasome system and autophagy in the clearance of soluble and aggregated TDP-43 species.
      Scotter, EL; Vance, C; Nishimura, AL; Lee, YB; Chen, HJ; Urwin, H; Sardone, V; Mitchell, JC; Rogelj, B; Rubinsztein, DC; Shaw, CE
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      Cancer cell death induced by novel small molecules degrading the TACC3 protein via the ubiquitin-proteasome pathway.
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      Cell death & disease 5 e1513 2014

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      Western Blotting25375378 25375378
      Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.
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