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07-024 | Anti-gp91-phox Antibody

07-024
200 µg  
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      Descripción

      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-024
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-gp91-phox Antibody
      Alternate Names
      • Cytochrome b(558) subunit beta
      • Cytochrome b558 subunit beta
      • Heme-binding membrane glycoprotein gp91phox
      • NADPH oxidase
      • Neutrophil cytochrome b 91 kDa polypeptide
      • Superoxide-generating NADPH oxidase heavy chain subunit
      • chronic granulomatous disease
      • cytochrome b-245 beta polypeptide
      • cytochrome b-245, beta polypeptide
      • p22 phagocyte B-cytochrome
      Background InformationCytochrome b-245, beta polypeptide (chronic granulomatous disease), also known as CYBB and P91-PHOX, is a human gene encoding a glycoprotein.
      Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles.
      References
      Product Information
      FormatPurified
      Control
      • Lung tissue, human neutrophil membrane, HL60 membrane and RIPA cell lysate.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.
      Applications
      ApplicationAnti-gp91-phox Antibody is an antibody against gp91-phox for use in WB.
      Key Applications
      • Western Blotting
      Application NotesImmunoprecipitation:
      Not recommended.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 548-560 of human gp91-phox (KQSISNSESGPRG). The immunizing sequence is identical in mouse, and it shares 11/13 sequence homology in bovine and 10/13 in porcine.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityDetects gp91-phox, Mr 75 kDa. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr 75 kDa, or appear as a smear between 75-91 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to cross react with mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
      Gene Symbol
      • CGD
      • CGD91-phox
      • EC 1.-.-.-
      • GP91-1
      • GP91-PHOX
      • GP91PHOX
      • NOX2
      • OTTHUMP00000031915
      • gp91-1
      • gp91-phox
      • p91-PHOX
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04839 # Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
      COFACTOR: FAD (Probable).
      SIZE: 570 amino acids; 65336 Da
      SUBUNIT: Composed of a heavy chain (beta) and a light chain (alpha).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      PTM: Glycosylated.
      DISEASE: SwissProt: P04839 # Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD) [MIM:306400]. X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections. Patients with CYBB dysfunction are generally classified into three groups, namely X91(0) (no expression of the protein); X91(-) (reduced expression) and X91(+) (normal expression).
      SIMILARITY: SwissProt: P04839 ## Contains 1 ferric oxidoreductase domain.
      Molecular WeightVaries. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr ~75 kDa, or appear as a smear between 75-91 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on human neutrophil membranes.

      Western Blot Analysis:
      1-4 µg/mL of this lot detected gp91-phox in a HL60 membrane and RIPA cell lysate. A previous lot detected gp91-phox in human neutrophil membranes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti- gp91-phox - DAM1491028 DAM1491028
      Anti-gp91-phox 2470971
      Anti-gp91-phox (rabbit polyclonal IgG) - 2197508 2197508
      Anti-gp91-phox - 2115689 2115689
      Anti-gp91-phox - 2387830 2387830
      Anti-gp91-phox - 0611045894 0611045894
      Anti-gp91-phox - 19435 19435
      Anti-gp91-phox - 2324396 2324396
      Anti-gp91-phox - 29583 29583
      Anti-gp91-phox - DAM1411286 DAM1411286
      Anti-gp91-phox - DAM1411286 DAM1411286
      Anti-gp91-phox - DAM1411286 DAM1411286
      Anti-gp91-phox - NG1508304 NG1508304
      Anti-gp91-phox - NG1508304 NG1508304
      Anti-gp91-phox -2578080 2578080
      Anti-gp91-phox -2742287 2742287

      Referencias bibliográficas | 14 Disponible | Ver todas las referencias

      Visión general referenciasAplicación Pub Med ID
      Acute ethanol induces apoptosis by stimulating TRPC6 via elevation of superoxide in oxygenated podocytes.
      Lu, XY; Liu, BC; Wang, LH; Yang, LL; Bao, Q; Zhai, YJ; Alli, AA; Thai, TL; Eaton, DC; Wang, WZ; Ma, HP
      Biochimica et biophysica acta 1853 965-74 2015

      Mostrar resumen
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      Fluvastatin attenuates hepatic steatosis-induced fibrogenesis in rats through inhibiting paracrine effect of hepatocyte on hepatic stellate cells.
      Chong, LW; Hsu, YC; Lee, TF; Lin, Y; Chiu, YT; Yang, KC; Wu, JC; Huang, YT
      BMC gastroenterology 15 22 2015

      Mostrar resumen
      25886887 25886887
      Lovastatin inhibits human B lymphoma cell proliferation by reducing intracellular ROS and TRPC6 expression.
      Song, X; Liu, BC; Lu, XY; Yang, LL; Zhai, YJ; Eaton, AF; Thai, TL; Eaton, DC; Ma, HP; Shen, BZ
      Biochimica et biophysica acta 1843 894-901 2014

      Mostrar resumen
      24518247 24518247
      Angiotensin Receptor Mediated Oxidative Stress Is Associated with Impaired Cardiac Redox Signaling and Mitochondrial Function in Insulin Resistant Rats.
      Vazquez-Medina, Jose Pablo, et al.
      Am. J. Physiol. Heart Circ. Physiol., (2013) 2013

      Mostrar resumen
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      Stress reaction in outer segments of photoreceptors after blue light irradiation.
      Roehlecke, C; Schumann, U; Ader, M; Brunssen, C; Bramke, S; Morawietz, H; Funk, RH
      PloS one 8 e71570 2013

      Mostrar resumen
      Western Blotting24039718 24039718
      Advanced oxidation protein products induce cardiomyocyte death via Nox2/Rac1/superoxide-dependent TRAF3IP2/JNK signaling.
      Valente, AJ; Yoshida, T; Clark, RA; Delafontaine, P; Siebenlist, U; Chandrasekar, B
      Free radical biology & medicine 60 125-35 2013

      Mostrar resumen
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      H2O2 production downstream of FLT3 is mediated by p22phox in the endoplasmic reticulum and is required for STAT5 signalling.
      Woolley, JF; Naughton, R; Stanicka, J; Gough, DR; Bhatt, L; Dickinson, BC; Chang, CJ; Cotter, TG
      PloS one 7 e34050 2012

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      NADPH oxidase mediates striatal neuronal injury after transient global cerebral ischemia.
      Yoshioka, H; Niizuma, K; Katsu, M; Okami, N; Sakata, H; Kim, GS; Narasimhan, P; Chan, PH
      Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 31 868-80 2011

      Mostrar resumen Artículo Texto completo
      20859296 20859296
      Angiotensin-II type 1 receptor and NOX2 mediate TCF/LEF and CREB dependent WISP1 induction and cardiomyocyte hypertrophy.
      Shanmugam, P; Valente, AJ; Prabhu, SD; Venkatesan, B; Yoshida, T; Delafontaine, P; Chandrasekar, B
      Journal of molecular and cellular cardiology 50 928-38 2011

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      Western Blotting21376054 21376054
      Redox survival signalling in retina-derived 661W cells.
      Mackey, AM; Sanvicens, N; Groeger, G; Doonan, F; Wallace, D; Cotter, TG
      Cell death and differentiation 15 1291-303 2008

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