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06-946 | Anti-hyperacetylated Histone H4 (Penta) Antibody

06-946
200 µL  
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      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, T, Eu ICC, WB, ChIP Rb Serum Polyclonal Antibody
      Description
      Catalogue Number06-946
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-hyperacetylated Histone H4 (Penta) Antibody
      Alternate Names
      • H4 histone family, member A
      • histone 1
      • H4a
      • histone cluster 1
      • H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      FormatSerum
      Control
      • Tetrahymena cells.
      PresentationRabbit polyclonal antiserum IgG in buffer containing 30% glycerol.
      Applications
      ApplicationUse Anti-hyperacetylated Histone H4 (Penta) Antibody (Rabbit Polyclonal Antibody) validated in ChIP, ICC, WB to detect hyperacetylated Histone H4 (Penta) also known as H4 histone family member A.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      Reported to immunoprecipitate transcriptionally active chromatin containing acetylated histone H4. (Perry, C.A., 1993)

      Immunocytochemistry:
      Reported to exclusively stain Tetrahymena macronuclei1 and chromatin in HeLa nuclei. (Perry, C.A., 1993)
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide
      (AGGAcKGGAcKGGAcKGGAcKGGAcKGGC) related to amino acids 2 to 19 of Tetrahymena thermophila histone H4.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes hyperacetylated histone H4, Mr 10 kDa (SDS-PAGE); tri- and tetra-acetylated histone H4 on acid-urea gels. May cross-react with other acetylated proteins.
      IsotypeIgG
      Species Reactivity
      • Human
      • Tetrahymena
      • Eukaryote
      Species Reactivity NoteHuman.
      Expected to cross-react with Tetrahymena and Eukaryote. Other species not tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • IST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular WeightApprox. 10 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HepG2 lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected HYPERACETYLATED HIS H4 on 10 μg of HepG2 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      Anti-Hyperacetylated Histone H4 (Penta) (rabbit antiserum) - 2377379 2377379
      Anti-hyperacetylated Histone H4 (Penta) - 2455674 2455674
      Anti-hyperacetylated Histone H4 (Penta) - 18966 18966
      Anti-hyperacetylated Histone H4 (Penta) - 26552 26552
      Anti-hyperacetylated Histone H4 (Penta) - 29860 29860
      Anti-hyperacetylated Histone H4 (Penta) -2631278 2631278

      Referencias bibliográficas | 42 Disponible | Ver todas las referencias

      Visión general referenciasAplicación EspeciePub Med ID
      Genome wide mapping of UBF binding-sites in mouse and human cell lines.
      Diesch, J; Hannan, RD; Sanij, E
      Genomics data 3 103-5 2015

      Mostrar resumen
      26484160 26484160
      A balance between activating and repressive histone modifications regulates cystic fibrosis transmembrane conductance regulator (CFTR) expression in vivo.
      Bergougnoux, A; Rivals, I; Liquori, A; Raynal, C; Varilh, J; Magalhães, M; Perez, MJ; Bigi, N; Des Georges, M; Chiron, R; Squalli-Houssaini, AS; Claustres, M; De Sario, A
      Epigenetics 9 1007-17 2014

      Mostrar resumen
      24782114 24782114
      CHD5 is required for spermiogenesis and chromatin condensation.
      Zhuang, T; Hess, RA; Kolla, V; Higashi, M; Raabe, TD; Brodeur, GM
      Mechanisms of development 131 35-46 2014

      Mostrar resumen
      Western Blotting24252660 24252660
      Histone hypoacetylation-activated genes are repressed by acetyl-CoA- and chromatin-mediated mechanism.
      Mehrotra, S; Galdieri, L; Zhang, T; Zhang, M; Pemberton, LF; Vancura, A
      Biochimica et biophysica acta 1839 751-63 2014

      Mostrar resumen
      24907648 24907648
      The yeast AMPK homolog SNF1 regulates acetyl coenzyme A homeostasis and histone acetylation.
      Zhang, M; Galdieri, L; Vancura, A
      Molecular and cellular biology 33 4701-17 2013

      Mostrar resumen
      24081331 24081331
      Elucidating combinatorial histone modifications and crosstalks by coupling histone-modifying enzyme with biotin ligase activity.
      Lau, PN; Cheung, P
      Nucleic acids research 41 e49 2013

      Mostrar resumen
      23258705 23258705
      Genomic occupancy of HLH, AP1 and Runx2 motifs within a nuclease sensitive site of the Runx2 gene.
      Hovhannisyan, H; Zhang, Y; Hassan, MQ; Wu, H; Glackin, C; Lian, JB; Stein, JL; Montecino, M; Stein, GS; van Wijnen, AJ
      Journal of cellular physiology 228 313-21 2013

      Mostrar resumen
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      Yeast phospholipase C is required for normal acetyl-CoA homeostasis and global histone acetylation.
      Galdieri, L; Chang, J; Mehrotra, S; Vancura, A
      The Journal of biological chemistry 288 27986-98 2013

      Mostrar resumen
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      Evidence for monomeric actin function in INO80 chromatin remodeling.
      Kapoor, P; Chen, M; Winkler, DD; Luger, K; Shen, X
      Nature structural & molecular biology 20 426-32 2013

      Mostrar resumen
      23524535 23524535
      Rhythmic oscillation of histone acetylation and methylation at the Arabidopsis central clock loci.
      Song, HR; Noh, YS
      Molecules and cells 34 279-87 2012

      Mostrar resumen
      22878891 22878891