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2170 | ChemiBLOCKER

2170
2 X 500 mL  sufficient for 100 mini gel blots
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      Descripción

      Replacement Information

      Tabla espec. clave

      Key Applications
      ELISA, WB, IHC
      Description
      Catalogue Number2170
      Brand Family Chemicon®
      Trade Name
      • ChemiBlock
      • Chemicon
      DescriptionChemiBLOCKER
      OverviewThe Chemicon ChemiBLOCKER™ is an unique membrane-blocking agent. As a non-animal protein blocker it offers an alternative to milk-based blocking agents, minimizing the risk of non-specific binding of antibodies to your membrane and lowering your background. ChemiBLOCKER™ may be used at dilutions as high as 20x but for most applications, a 5 to 10 fold dilution is appropriate. ChemiBLOCKER™ is biotin free and will not interfere with ABC detection systems. ChemiBLOCKER™ does contain phosphates and may not be suitable for use with some phospho-specific antibodies.

      Related Products:
      • BLOT-QuickBlocker™ A novel blocking protein which produces a semi-clear solution. This allows users the option to examine their blot membranes during the blocking procedure. 200g Catalog Number 2080
      • Femto/TBST wash buffer (10X) 250mL Catalog Number 2081

      Materials required:
      • 1X femto/TBST (Catalog Number 2081), 1X TBST, or 1X PBST
      • primary and secondary antibodies
      • reagents for immunodetection
      References
      Product Information
      Components
      • 2X ChemiBLOCKER 1 L
      PresentationA 2X solution of non-animal proteins in 2mM TRIS, pH 8.5, 100mM NaCl, 0.001% Tween-20, and 0.08% Kathon added as a preservative.
      Applications
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot

      ELISA

      PROTOCOL:

      1. Make up blocking solution by mixing 1 part ChemiBLOCKER™ with 1 part 1X femto-TBST/PBST (TBST or PBST also work) for blocking PVDF membranes. For nitrocellulose membranes, mix 1 part ChemiBLOCKER™ with 3 parts 1X femto-TBST (TBST or PBST also work). Note:ChemiBLOCKER™ can be used at a wide variety of dilutions, some optimization may be necessary. ChemiBLOCKER™ is NOT recommended for phospho-protein studies as it does contain phosphorylated proteins.

      2. Incubate the membrane in blocking solution (10-20 mL) for 60 minutes at room temperature with shaking in a heat-sealable plastic bag.

      3. Prepare antibody dilution buffer (ADB). For PVDF membranes mix 1 part ChemiBLOCKER™ with 3 parts 1X femto-TBST/PBST (TBST or PBST also work). Incubate the membrane in 5-10 mL primary antibody, diluted appropriately with ADB, for 90 min at room temperature with shaking.

      4. Wash the membrane 3 times in 50 mL of 1X femto-TBST (TBST or PBST also work) for 5 min each time at room temperature with shaking.

      5. Incubate the membrane in 5-10 mL secondary antibody, diluted appropriately with ADB, for 90 min at room temperature with shaking.

      6. Wash the membrane as in step #4, but add one extra wash (4 washes total).

      7. Wash the membrane 3 times quickly (30 sec.) in 50 mL pure deionized water each time and then process the blot, using femto-LUCENT™ detection system (or your favorite system).
      Biological Information
      Physicochemical Information
      Dimensions
      Volume1 L
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore kit components at 2-8°C for up to 1 year from date of purchase. Warm ChemiBLOCKER™ to room temperature and mix thoroughly before dispensing. To avoid possible microbial contamination, dispense all solutions aseptically. Over time, phenolic compounds may precipitate out of solution resulting in the formation of a small amount of dark brown to black particulate material at the bottom of the container. This material will not affect the performance of the blocking compound.
      Packaging Information
      Material Size2 X 500 mL
      Material Packagesufficient for 100 mini gel blots
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      ChemiBLOCKER TM - 2055959 2055959
      ChemiBLOCKERTM 2470376
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS - 2390359 2390359
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS - 2373305 2373305
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS - 2325027 2325027
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS -2483221 2483221
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS -2526724 2526724
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS -2558392 2558392
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS -2620626 2620626
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS -2653124 2653124
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS -2698371 2698371
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS -2714552 2714552
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS -2776831 2776831
      ChemiBLOCKERTM A new Non-animal Protein for your Immuno-assays - 2151808 2151808
      ChemiBLOCKER™ -2736644 2736644

      Referencias bibliográficas

      Visión general referenciasAplicación Pub Med ID
      Suppression of non-specific binding in serological Luminex assays
      Tim Waterboer *, Peter Sehr 1, Michael Pawlita, Infection and Cancer Program, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 242, 69120 Heidelberg, Germany
      Journal of Immunological Methods 309 (2006) 200– 204 2006

      Multiplexing
      Multiplex human papillomavirus serology based on in situ-purified glutathione s-transferase fusion proteins
      Waterboer, Tim, et al
      Clin Chem, 51:1845-53 (2005) 2005

      16099939 16099939
      Widespread thalamic terminations of fibers arising in the superficial medullary dorsal horn of monkeys and their relation to calbindin immunoreactivity.
      Graziano, Alessandro and Jones, Edward G
      J. Neurosci., 24: 248-56 (2004) 2004

      Mostrar resumen
      14715957 14715957

      Ficha técnica

      Cargo
      ChemiBLOCKERTM A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS
      ChemiBLOCKER™ A NEW NON-ANIMAL PROTEIN FOR YOUR IMMUNO-ASSAYS