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251515 | DAF-FM - CAS 254109-20-1 - Calbiochem

251515
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      Descripción

      Replacement Information

      Tabla espec. clave

      Empirical FormulaCAS #
      C₂₁H₁₄F₂N₂O₅ 254109-20-1

      Precios y disponibilidad

      Número de referenciaDisponiblidad Embalaje Cant./Env. Precio Cantidad
      US1251515-1MG
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          Frasco de vidrio 1 mg
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          Description
          OverviewA photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO in the presence of O2, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8.
          Catalogue Number251515
          Brand Family Calbiochem®
          Synonyms3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein
          References
          ReferencesKojima, H., et al. 2001. J. Neurochem. 76, 1404.
          Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
          Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
          Kojima, H., et al. 1999. Angew. Chem. Int. Ed. 38, 3209.
          Product Information
          CAS number254109-20-1
          ATP CompetitiveN
          DeclarationManufactured by Daiichi Pure Chemicals Co., Ltd
          FormPale yellow solution
          FormulationIn DMSO.
          Hill FormulaC₂₁H₁₄F₂N₂O₅
          Chemical formulaC₂₁H₁₄F₂N₂O₅
          ReversibleN
          Structure formula Image
          Applications
          Biological Information
          Primary TargetNitric oxide (NO) fluorescent indicator
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableN
          Emission max.
          Excitation max.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/38

          Irritating to eyes and skin.
          S PhraseS: 26

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze at -20°C. Use immediately after dilution, discard any unused portion.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          Certificados de análisis

          CargoNúmero de lote
          251515

          Referencias bibliográficas

          Visión general referencias
          Kojima, H., et al. 2001. J. Neurochem. 76, 1404.
          Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
          Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
          Kojima, H., et al. 1999. Angew. Chem. Int. Ed. 38, 3209.
          Ficha técnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-April-2011 RFH
          Synonyms3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein
          DescriptionA photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.
          FormPale yellow solution
          FormulationIn DMSO.
          Intert gas (Yes/No) Packaged under inert gas
          Recommended reaction conditionsThe reagent is provided at ~7 mM concentration. Prepare a 1000-fold dilution in 0.1 M phosphate buffer, pH 7.4 before use. BSA and phenol red may affect fluorescence.
          CAS number254109-20-1
          Chemical formulaC₂₁H₁₄F₂N₂O₅
          Structure formula
          Purity≥98% by HPLC
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze at -20°C. Use immediately after dilution, discard any unused portion.
          Toxicity Irritant
          ReferencesKojima, H., et al. 2001. J. Neurochem. 76, 1404.
          Park. J.M., et al. 2001. Br. J. Pharmacol. 132, 1876.
          Itoh, Y., et al. 2000. Anal. Biochem. 287, 203.
          Kojima, H., et al. 1999. Angew. Chem. Int. Ed. 38, 3209.