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70967 | GeneJuice® Transfection Reagent

70967
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      US170967-4
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              Frasco de vidrio 0.3 ml
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              US170967-6
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                  Frasco de vidrio 5 ml
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                  Cell lines transfected with GeneJuice® Transfection Reagent
                  Description
                  Overview""I use GeneJuice because it's ease-of-use, low toxicity and wide range of target cells""
                  Stuart Rulten. Research Fellow. University of Sussex

                  ""Since we have been working with GeneJuice, we have saved a lot of time and money!""
                  Dr. Andrea Kress, Institute of Clinical and Molecular Virology, Erlangen, Germany

                  ""I find that Genejuice works for a multitude of cell types with minimal optimization""
                  Caitriona Marie Lyons. PhD Student. University College Cork, Ireland

                  Request a Free Sample

                  Transfection is the process by which nucleic acids are introduced into mammalian cells. GeneJuice Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity for mammalian cells. Whereas many available transfection reagents are based on cationic lipid formulation, GeneJuice Transfection Reagent is composed of a nontoxic cellular protein and a small amount of a novel polyamine. GeneJuice Transfection Reagent enables highly efficient DNA transfer in both stable and transient transfections of eukaryotic cells and is ideal for high-throughput transfections in a multi-well plate format. The unique composition is compatible with both serum-containing and serum-free media, making media changes unnecessary during transfection experiments. Genejuice is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, lipofection, and complex formation with DEAE-dextran. The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates.







                  Cell Lines:Primary Cells:
                  10T1/2
                  293T
                  3T3 NIH
                  3T3 Swiss
                  3T3-L1
                  A204
                  A431
                  A549
                  alpha TC1-6
                  AR 42J
                  As4.1
                  AtT-20
                  B50
                  BC-1
                  BC-2
                  BC3
                  BCBL
                  BHK-21
                  C3H/10T1/2
                  C6
                  C2C12
                  Caco-2
                  Caki-1
                  Calpan-1
                  Calu-1
                  Calu-6
                  CCL-131
                  CFPAC-1
                  Chang Liver
                  CHO
                  CHO-7
                  CHO-IR
                  CHO-K1
                  COS-1
                  COS-7
                  CS-1
                  CV-1
                  Daudi
                  DDTI MF-2
                  DT40
                  ECV304
                  EL4
                  ES-E14TG2a
                  EVSCC17M
                  H9c2
                  HCT-116
                  HEK293
                  HeLa
                  HeLa B
                  HeLa T4
                  Hep 3B2.1-7
                  HepG2
                  Hepa 1-6
                  Ht-29
                  HTB-37
                  HTB-45
                  Huh-7
                  HUVEC
                  IC21
                  IEC-6
                  JEG-3
                  Jurkat
                  KB
                  L57-3-11
                  L-6
                  L-929
                  MA-10
                  McA-RH7777
                  MCF-7
                  MCF-10-2A
                  MDCK
                  Melanocyte
                  MG-63
                  Neuro 2A
                  Neuroblastoma
                  NRK
                  NT2/D1
                  OV-1063
                  OVCAR3
                  P4
                  P19
                  PC12
                  PA317
                  PAM212
                  PS-1
                  R2C
                  RAW 264.7
                  RBL-2H3
                  RMP-41
                  SAOS-2
                  SC-1
                  Schneider line2
                  SK-N-MC
                  SK-N-SH
                  SKOV3
                  STO
                  SW-480
                  SW-837
                  T3M4
                  TM4
                  U937
                  UCD
                  Vero
                  Aortic smooth muscle cells
                  Astrocytes
                  Angioblasts
                  Chondrocytes
                  Chromaffin cells
                  Epithelial cells:
                    mammary
                    prostate
                    tracheal
                  Fibroblasts
                  Keratinocytes
                  Catalogue Number70967 Brand Family Novagen®
                  Features and benefits
                  • Highly efficient DNA transfer for both stable and transient transfections
                  • Minimal cellular toxicity
                  • Compatibility with both serum-containing and serum-free media
                  • Simple protocol—no need for media changes
                  • Ideal for high-throughput transfection in a multi-well plate format


                  References
                  References
                • Koleva, R.I., et al. 2006. J. Biol. Chem. 281, 25110.
                • Product Information
                  DeclarationGeneJuice® is a registered trademark of EMD Chemicals Inc.
                  Applications
                  Biological Information
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  R PhraseR: 11

                  Highly flammable.
                  S PhraseS: 7-16

                  Keep container tightly closed.
                  Keep away from sources of ignition - No Smoking.
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Blue Ice Only
                  Toxicity Flammable
                  Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
                  Storage +2°C to +8°C
                  Do not freeze Ok to freeze
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  Ficha datos de seguridad (MSDS)

                  Título

                  Ficha técnica de seguridad del material (MSDS) 

                  Certificados de análisis

                  CargoNúmero de lote
                  70967

                  Referencias bibliográficas

                  Visión general referencias
                • Koleva, R.I., et al. 2006. J. Biol. Chem. 281, 25110.
                • Folleto

                  Cargo
                  Nano Juice™ Transfection Kit

                  Citas

                  Título
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                • Ogasawara, Y., et al. 2008. J. Biol. Chem. 283, 8885.
                • Satoh, T., et al. 2008. Cell 132, 935.
                • Wood R.J., and Hulett M.D., 2008. Journal of Biological Chemistry 283, 4165.
                • Yokouchi, M., et al. 2008. Journal of Biological Chemistry 283, 4252.
                • Buckley, N.E., et al. 2007. Molecular Cancer Research 5, 261.
                • Christova, R., et al. 2007. Journal of Cell Science 120, 3262.
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                • Lee, J.Y., et al. 2007. Molecular Pharmacology 72, 1082.
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                • Crimaldi, et al., 2009. Experimental Cell Research 315, 2581.
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                • D'Arcy, P., et al. 2008. Molecular Cancer Research 6, 127.
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                • Ozbay, T., et al. 2006. Endocrinology 14, 1427.
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                • Severa, M., et al. 2006. Journal of Biological Chemistry 281, 26188.
                • Stafford, J.L., et al. 2006. Journal of Immunology 177, 2505.
                • Tong, E.H.Y., et al. 2006. J. Biol. Chem. 281, 23870.
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                • Chatterjee, P., et al. 2011. J. Vis. Exp. 56, 3110.
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                • Crimaldi, et al., 2009. Experimental Cell Research 315, 2581.
                • Sanderson, L.M., et al. 2009. Mol. Cell. Biol. 29, 6257.
                • Athie-Morales, V., et al. 2008. Journal of Immunology 180, 4082.
                • D'Arcy, P., et al. 2008. Molecular Cancer Research 6, 127.
                • Ho, G.T.T., et al. 2008. J. Biol. Chem. 283, 21588.
                • Morris, D.P., et al. 2008. Journal of Biological Chemistry 283, 2973.
                • Ogasawara, Y., et al. 2008. J. Biol. Chem. 283, 8885.
                • Satoh, T., et al. 2008. Cell 132, 935.
                • Wood R.J., and Hulett M.D., 2008. Journal of Biological Chemistry 283, 4165.
                • Yokouchi, M., et al. 2008. Journal of Biological Chemistry 283, 4252.
                • Buckley, N.E., et al. 2007. Molecular Cancer Research 5, 261.
                • Christova, R., et al. 2007. Journal of Cell Science 120, 3262.
                • Herzog, S., and Jumaa, H., 2007. Journal of immunology 178, 2336.
                • Leo T. O. Lee, et al. 2007. Procedings of the National Academy of Science 104, 2133.
                • Lee, J.Y., et al. 2007. Molecular Pharmacology 72, 1082.
                • Miggin, S.M., et al. 2007. Procedings of the National Academy of Science 104, 3372.
                • Qiu, J., et al. 2007. Molecular Biology of the Cell 18, 3941.
                • Row, P.E., et al. 2007. J. Biol. Chem. 282, 30929.
                • Schauwaers, K., et al. 2007. Procedings of the National Academy of Science 104, 4961.
                • Wu, C., and Huang, J., 2007. Journal of Biological Chemistry 282, 3571.
                • Adrain, C., et al. 2006. Journal of Biological Chemistry 281, 8118.
                • Atkinson, P.J., et al. 2006. Molecular Pharmacology 69, 174.
                • Baens, M., et al. 2006. Cancer Research 66, 5270.
                • Dastur, A., et al. 2006. Journal of Biological Chemistry 281, 4334.
                • Fichorova, R.N., et al. 2006. Infection and Immunity 74, 5773.
                • Goldring, C., et al. 2006. American Journal of Physiology: Cell 290, C104.
                • Gopalan, S.M., et al. 2006. Journal of Biological Chemistry 281, 13126.
                • Henderson, M.J., et al. 2006. J. Biol. Chem. 281, 39990.
                • Hong, Yunhan and Schartl, Manfred 2006. Methods Mol Biol. 329, 3.
                • Koleva, R.I., et al. 2006. J. Biol. Chem. 281, 25110.
                • Massari, P., et al. 2006.Journal of Immunology 176, 2373.
                • Muthukumaran, N., et al. 2006. Molecular Cancer Research 4, 511.
                • Ozbay, T., et al. 2006. Endocrinology 14, 1427.
                • Schneider, M.E, et al. 2006. Journal of Neuroscience 26, 10243.
                • Severa, M., et al. 2006. Journal of Biological Chemistry 281, 26188.
                • Stafford, J.L., et al. 2006. Journal of Immunology 177, 2505.
                • Tong, E.H.Y., et al. 2006. J. Biol. Chem. 281, 23870.
                • Tooze, R.M., et al. 2006. Journal of Immunology 177, 4584.
                • Xin Yang, et al. 2006. Am. J. Physiol. Heart Circ. Physiol. 291, H2334.
                • Bang, R., et al. 2005. Journal of Biological Chemistry 280, 25095.
                • Bartlett, P.J., et al. 2005. Journal of Biological Chemistry 280, 21837.
                • Bjorkbacka, H., et al. 2005. Physiological Genomics 19, 319.
                • Boyer-Guittaut, M., et al. 2005. Journal of Biological Chemistry 280, 9937.
                • Brady, M., et al. 2005. Molecular and Cellular Biology 25, 545.
                • Brady, G., et al. 2005. Journal of Biological Chemistry 280, 30723.
                • Brockington, M., et al. 2005. Human Molecular Genetics 14, 657.
                • Butler, M.P., et al. 2005. Journal of Biological Chemistry 280, 27759.
                • Cant S.H., and Pitcher, J.A., 2005. Molecular Biology of the Cell 16, 3088.
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                • Pos, Z., et al. 20
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