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FC010 | Human Plasma Fibronectin Purified Protein

FC010
1 mg  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Key Applications
      CULT
      Description
      Catalogue NumberFC010
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHuman Plasma Fibronectin Purified Protein
      OverviewHuman fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.
      References
      Product Information
      PresentationLiquid in 150 mM NaCl, 10 mM sodium phosphate, pH 7.5, sterile filtered, containing no preservatives.
      Applications
      ApplicationThis Human plasma fibronectin is a purified protein, used as an attachment factor suitable for cell propagation in vitro.
      Key Applications
      • Cell Culture
      Application NotesImmunochemical standard

      Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts

      Suggested Procedure for Coating Cell Cultureware

      1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.

      2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

      3.Introduce the proper CO2 atmosphere, if required.

      4.Add the calculated amount of HFN to each culture vessel.

      5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.

      6.Remove residual balanced salt solution before proceeding with standard cell culture procedures
      Biological Information
      Concentration1 mg/mL
      PurityApproximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. hFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose. Plasma from donors has been screened, and shown to be negative for HIV, HTLV, Hepatitis B and C.
      SourceHuman donor plasma.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
      Gene Symbol
      • FN1
      • DKFZp686O13149
      • DKFZp686H0342
      • DKFZp686I1370
      • MSF
      • FN
      • CIG
      • DKFZp686F10164
      • LETS
      • FINC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
      SIZE: 2386 amino acids; 262607 Da
      SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
      TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
      PTM: Sulfated.
      SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up to 6 months from date of receipt. Do not freeze.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      HUMAN PLASMA FIBRONECTIN 2459009
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2390363 2390363
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2426452 2426452
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2428450 2428450
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2444037 2444037
      HUMAN PLASMA FIBRONECTIN - 2493933 2493933
      HUMAN PLASMA FIBRONECTIN -2549971 2549971
      HUMAN PLASMA FIBRONECTIN -2585886 2585886
      HUMAN PLASMA FIBRONECTIN -2645417 2645417
      HUMAN PLASMA FIBRONECTIN -2676512 2676512
      HUMAN PLASMA FIBRONECTIN -2709155 2709155
      HUMAN PLASMA FIBRONECTIN -2709156 2709156
      HUMAN PLASMA FIBRONECTIN -2736665 2736665
      HUMAN PLASMA FIBRONECTIN -2742714 2742714
      HUMAN PLASMA FIBRONECTIN -2796371 2796371
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2063139 2063139
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2090103 2090103
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2186322 2186322
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2272678 2272678
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2324496 2324496
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2326977 2326977
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2361790 2361790
      HUMAN PLASMA FIBRONECTIN, PURIFIED PROTEIN 2838759

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Intestinal epithelial wound healing assay in an epithelial-mesenchymal co-culture system.
      Seltana, Amira, et al.
      Wound Repair Regen, 18: 114-22 2010

      Mostrar resumen
      20082684 20082684
      Integrin activation by dithiothreitol or Mn2+ induces a ligand-occupied conformation and exposure of a novel NH2-terminal regulatory site on the beta1 integrin chain.
      Ni, H, et al.
      J. Biol. Chem., 273: 7981-7 (1998) 1998

      Mostrar resumen
      9525896 9525896
      Comparative studies on amniotic fluid and plasma fibronectins
      Ruoslahti E. et al.
      Biochem. J, 193:295-299 (1981) 1981

      7305927 7305927

      Folleto

      Cargo
      Human Stem Cell Systems

      Ficha técnica

      Cargo
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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