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FC010-100MG | Human Plasma Fibronectin Purified Protein

FC010-100MG
100 mg  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Key Applications
      CULT
      Description
      Catalogue NumberFC010-100MG
      ReplacesFC010-250MG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHuman Plasma Fibronectin Purified Protein
      OverviewHuman fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.
      References
      Product Information
      PresentationLyophilized from 150 mM NaCl, 10 mM sodium phosphate, pH 7.5, containing no preservatives.
      Applications
      Key Applications
      • Cell Culture
      Application NotesImmunochemical standard

      Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts

      Suggested Procedure for Coating Cell Cultureware

      1. Determine the amount of hFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (mg/mL) of hFN. Recommended amount is 2-10 mg/cm2.

      2. Reconstitute the entire vial* of hFN to 1mg/mL using distilled or deionized water at 37ºC. Add the lyophilized hFN slowly with constant stirring, and avoid excessive foaming. Break up any clumps that might form against the side of the container with a glass rod or spatula. Sterile filter the hFN solution to remove any remaining insoluble material. Store the reconstituted material at 2º-8° C for up to 6 months. For working dilutions, dilute the material to the desired concentration with physiological buffer. For determining final protein concentrations by OD280, the Extinction coefficient = 1.3 [(mg/mL)xcm]-1).

      3. Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

      4. Introduce the proper CO2 atmosphere, if required.

      5. Add the calculated amount of hFN to each culture vessel and store the remaining hFN at 2º-8ºC as indicated below.

      6. Allow hFN to adsorb to the surface of the vessel for 5-20 minutes.

      7. Remove residual balanced salt solution before proceeding with standard cell culture procedures.



      *Note: hFN is lyophilized from buffered saline and the weight of the material is not only of the protein itself, but includes the protein + salts. The material may not be homogeneous with regard to protein/salt ratio.
      Biological Information
      PurityApproximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. HFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.
      SourceHuman donor plasma.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
      Gene Symbol
      • FN1
      • DKFZp686O13149
      • DKFZp686H0342
      • DKFZp686I1370
      • MSF
      • FN
      • CIG
      • DKFZp686F10164
      • LETS
      • FINC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
      SIZE: 2386 amino acids; 262607 Da
      SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
      TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
      PTM: Sulfated.
      SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months from date of receipt. Store the reconstituted material at 2º-8° C for up to 6 months.
      Packaging Information
      Material Size100 mg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Certificados de análisis

      CargoNúmero de lote
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2390364 2390364
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2024988 2024988
      HUMAN PLASMA FIBRONECTIN -2688666 2688666
      HUMAN PLASMA FIBRONECTIN -2739397 2739397
      HUMAN PLASMA FIBRONECTIN -2762386 2762386
      HUMAN PLASMA FIBRONECTIN -2789581 2789581
      HUMAN PLASMA FIBRONECTIN -2825434 2825434
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN -
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2189689 2189689
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN - 2366198 2366198
      HUMAN PLASMA FIBRONECTIN PURIFIED PROTEIN -2475638 2475638

      Referencias bibliográficas

      Visión general referenciasPub Med ID
      Intestinal epithelial wound healing assay in an epithelial-mesenchymal co-culture system.
      Seltana, Amira, et al.
      Wound Repair Regen, 18: 114-22 2010

      Mostrar resumen
      20082684 20082684
      Molecular properties of wild-type and mutant betaIG-H3 proteins
      Kim, Jung-Eun, et al
      Invest Ophthalmol Vis Sci, 43:656-661 (2002) 2002

      11867580 11867580
      Comparative studies on amniotic fluid and plasma fibronectins
      Ruoslahti E. et al.
      Biochem. J, 193:295-299 (1981) 1981

      7305927 7305927

      Folleto

      Cargo
      Human Stem Cell Systems

      Ficha técnica

      Cargo
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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      Life Science Research > Proteins and Enzymes > ECM Proteins for Plate Coating