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429705 | Leptin, Mouse, Recombinant, E. coli

429705
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      Descripción

      Replacement Information

      Precios y disponibilidad

      Número de referenciaDisponiblidad Embalaje Cant./Env. Precio Cantidad
      US1429705-5MG
      Comprobando disponibilidad...
      Disponibilidad a confirmarDisponibilidad a confirmar
      En existencia 
      Suspendido
      Cantidades limitadas disponibles
      Debe confirmarse disponibilidad
        El resto: se avisará
          El resto: se avisará
          Se avisará
          Póngase en contacto con el Servicio de Atención al Cliente

          Frasco de vidrio 5 mg
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          Description
          OverviewRecombinant, mouse leptin espressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations in the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. Suppresses insulin secretion by inhibiting activities of Ca2+-dependent PKC isoforms. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis.
          Catalogue Number429705
          Brand Family Calbiochem®
          SynonymsβrmOB
          References
          ReferencesAnderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
          Ookuma, M., et al. 1998. Diabetes 47, 219.
          Campfield, L.A., et al. 1995. Science 269, 546.
          Halaas, J.L., et al. 1995. Science 269, 543.
          Pelleymounter, M.A., et al. 1995. Science 269, 540.
          Zhang, Y., et al. 1994. Nature 372, 425.
          Product Information
          CAS number181030-10-4
          FormLyophilized
          FormulationLyophilized from a sterile filtered PBS.
          Applications
          Biological Information
          Biological activityED₅₀ = 0.2-1 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells
          Purity≥97% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: ≤1.0 EU/µg leptin
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          Certificados de análisis

          CargoNúmero de lote
          429705

          Referencias bibliográficas

          Visión general referencias
          Anderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
          Ookuma, M., et al. 1998. Diabetes 47, 219.
          Campfield, L.A., et al. 1995. Science 269, 546.
          Halaas, J.L., et al. 1995. Science 269, 543.
          Pelleymounter, M.A., et al. 1995. Science 269, 540.
          Zhang, Y., et al. 1994. Nature 372, 425.
          Ficha técnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision26-March-2009 JSW
          SynonymsβrmOB
          DescriptionRecombinant, mouse leptin espressed in E. coli. Leptin was originally identified as a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding leptin have been isolated from human, simian, mouse and rat cells. Human leptin shares ~84% sequence identity with the mouse protein. Human leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid signal sequence that is cleaved to yield the 146 amino acid mature protein. The expression of leptin mRNA has been shown to be restricted to adipose tissue.

          A high-affinity receptor for leptin (OB-R) with homology to gp130 and the G-CSF receptor was subsequently cloned. The OB-R cytoplasmic domain transduces the leptin signal through the JAK-STAT pathway. Although OB-R mRNA was initially shown to be expressed predominantly in the choroid plexus and in the hypothalamus, more recent data also revealed the expression of this receptor in endothelial cells (Ecs). Furthermore, the angiogenic activity of leptin has been demonstrated both in vitro and in vivo, suggesting a physical mechanism whereby leptin-induced angiogenesis may facilitate increased energy expenditure.
          FormLyophilized
          FormulationLyophilized from a sterile filtered PBS.
          CAS number181030-10-4
          Purity≥97% by SDS-PAGE
          ContaminantsEndotoxin: ≤1.0 EU/µg leptin
          Biological activityED₅₀ = 0.2-1 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells
          SolubilityReconstitute with 20 mM Tris-HCl, pH 8.0 to a final concentration of 1 mg/ml.
          Storage ≤ -70°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          Merck USA index14, 5443
          ReferencesAnderwald, C., et al. 2002. Mol. Endocrinol. 16, 1612.
          Ookuma, M., et al. 1998. Diabetes 47, 219.
          Campfield, L.A., et al. 1995. Science 269, 546.
          Halaas, J.L., et al. 1995. Science 269, 543.
          Pelleymounter, M.A., et al. 1995. Science 269, 540.
          Zhang, Y., et al. 1994. Nature 372, 425.