A part of Merck

HGPMAG-27K | MILLIPLEX MAP Human Glycolysis Pathway Magnetic Bead Panel - Cellular Metabolism Multiplex Assay

HGPMAG-27K
Diseñe y calcule el precio de su kit
Click To Print This Page

Descripción

Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
ENO1, G6PI, HIF-1α, LDHA, LDHB, PKM2, TKT H Mplex Luminex xMAP
Description
Catalogue NumberHGPMAG-27K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Glycolysis Pathway Magnetic Bead Panel - Cellular Metabolism Multiplex Assay
OverviewThe MILLIPLEX® MAP Human Glycolysis Pathway Magnetic Bead Panel (HGPMAG-27K). This panel is to be used for the simultaneous quantification of the following 7 analytes in any combination in cell or tissue lysates:
• ENO1: Enolase 1, α-enolase, C-myc promoter-binding protein, non-neural enolase
• G6PI: Glucose-6-phosphate isomerase, GPI
• HIF-1α: Hypoxia-inducible factor 1-α, ARNT-interacting protein
• LDHA: L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-M
• LDHB: L-lactate dehydrogenase B chain, LDH heart subunit, LDH-H
• PKM2: Pyruvate kinase isoform M2, Pyruvate kinase isozyme M2
• TKT: Transketolase, TK
Background InformationThe glycolysis pathway is a major energy source for the cell and is responsible for the conversion of glucose into pyruvate. The resulting pyruvate can then be converted into lactic acid in the cytoplasm or transferred to the mitochondria where it is converted into Acetyl-CoA which can enter the Krebs cycle and oxidative phosphorylation, generating ATP. Increased glucose uptake and a shift to aerobic glycolysis have been observed in many human tumors. This phenomenon is known as the Warburg effect. Glycolysis-related genes are highly upregulated genes in cancer. Monitoring and targeting metabolism in cancer cells is a rapidly expanding area of research, and deregulation of cellular energetics is now included as an emerging hallmark of cancer.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <15%
  • Intra-assay: <10%
Panel TypeMAGNETIC Cellular Metabolism
Applications
ApplicationCellular Metabolism Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple oxidative phosphorylation proteins in tissue and cell lysate samples.
Key Applications
  • Multiplexing
Application Notes• Two hours incubation is recommended for best results.
• These assays require 25 µL diluted cell or tissue lysate.
• We recommend using 1-10 µg total protein/well.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in each panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • ENO1
  • G6PI
  • HIF-1α
  • LDHA
  • LDHB
  • PKM2
  • TKT
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within this panel.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Human Glycolysis Pathway Magnetic Bead Panel

Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Folleto

Cargo
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Immunoassay and Multiplex Kits: Analyte Quarterly Vol. 2, 2016
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Licencias necesarias e Información técnica

Cargo
96 well Template 2 Up
96 well Template Single

Ficha técnica

Cargo
Full Service Custom-built Assays

Póster

Cargo
MILLIPLEX MAP: Exercise, Signaling, and Your Brain
Biomarkers of the tumor mIcroenvironment
Cancer & Metastasis Signaling Pathways
Multiplex Assays of Fifteen Human Complement Factors