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HSP5MAG-63K | MILLIPLEX MAP Human Sepsis Magnetic Bead Panel 5 - Immune Response Multiplex Assay

HSP5MAG-63K
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Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
PRTN-3, ELA-2 H Mplex Luminex xMAP
Description
Catalogue NumberHSP5MAG-63K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Sepsis Magnetic Bead Panel 5 - Immune Response Multiplex Assay
Background InformationSepsis or SIRS (systemic inflammatory response syndrome) results from infection, whether bacterial, viral, fungal or parasitic. According to the CDC, sepsis is the second leading cause of death in non-coronary ICU patients in the U.S. This hyper-reaction to infection interrupts homeostasis through an uncontrolled inflammatory response, including glucocorticoids and catecholamines, mediators of the humoral immune response, as well as pro-inflammatory cytokines. Severe sepsis occurs when hypotension or hypoperfusion to one or more organs leads to organ dysfunction, which, in turn, can cause septic shock, multiple organ dysfunction syndrome (MODS) and death.

Apoptosis plays a critical role in the development of severe sepsis and MODS. Studies involving critically ill patients have demonstrated that increased apoptosis of lymphoid organs and other organ tissues contributes to immune suppression, the body’s failure to respond to an antigen and organ dysfunction. The same pro-inflammatory cytokines and humoral mediators also contribute to the development of acquired immune defects. During sepsis, lymphocyte apoptosis can be initiated by the release of glucocorticoids and “death” cytokines. Understanding this has caused a shift in interest toward therapies aimed at inhibiting apoptosis and reversing immune suppression.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15
  • Intra-assay: < 10
Panel TypeMAGNETIC Immune Response
Applications
ApplicationInflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple sepsis biomarkers in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application NotesThe preferred assay is an overnight assay (4 ºC) requiring 25 μL of 1:100 diluted serum or plasma samples or 25 μL of tissue or cell culture supernatant. Plasma is preferred over serum. When serum is used, it is recommended that serum be used throughout the study to maintain consistency.Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for the analytes in this panel.
Species Reactivity
  • Human
Analytes Available
  • PRTN-3
  • ELA-2
Accuracy
  • 84% - 101%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for individual sensitivites.
Standard Curve Range
  • PRTN-3: 0.012-50 ng/mL
  • ELA-2: 0.002-10 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Human Sepsis Magnetic Bead Panel 5

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Título

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Folleto

Cargo
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Immunoassay and Multiplex Kits: Analyte Quarterly Vol. 2, 2016
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Licencias necesarias e Información técnica

Cargo
96 well Template 2 Up
96 well Template Single
Quality Control Ranges: Milliplex Human Sepsis Panel 5

Ficha técnica

Cargo
Full Service Custom-built Assays

Póster

Cargo
MILLIPLEX® MAP Cell Signaling Wall Chart (MM)
Multiplex Assays of Fifteen Human Complement Factors