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MCYP2MAG-73K | MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II - Immunology Multiplex Assay

MCYP2MAG-73K
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Descripción

Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IL-21, IL-22, IL-25, IL-28B, MCP-5, MIP-3α/CCL20, MIP-3β, TARC M Mplex Luminex xMAP
Description
Catalogue NumberMCYP2MAG-73K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II - Immunology Multiplex Assay
OverviewTo identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results comparable to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing

Therefore, the MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Background Information“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: 1 - 4
  • Inter-assay: 6 - 14
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationThe cytokine analytes available for this multiplex kit are: IL-21, IL-22, IL-25, IL-28B, MCP-5, MIP-3α/CCL20, MIP-3β, TARC.
Key Applications
  • Multiplexing
Application Notes• This kit may be used for the analysis of all or any combination of the cytokines and chemokines in serum, plasma or tissue/cell lysate and culture supernatant samples.

• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
o This assay requires 25 µl of 1:1 diluted plasma or serum, or 25 μL cell culture supernatant per well.
Biological Information
Species Reactivity
  • Mouse
Analytes Available
  • IL-21
  • IL-22
  • IL-25
  • IL-28B
  • MCP-5
  • MIP-3α/CCL20
  • MIP-3β
  • TARC
Assay PanelEverything you need in a single kit.
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • 94%–108%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual cytokines/chemokines.
Standard Curve Range
  • EPO: 16 – 50,000 pg/mL
  • All other analytes: 3.2 - 10,000
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Mouse Cytokine/Chemokine Magnetic Bead Panel II

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Referencias bibliográficas

Visión general referenciasPub Med ID
Primary breast cancer stem-like cells metastasise to bone, switch phenotype and acquire a bone tropism signature.
D'Amico, L; Patanè, S; Grange, C; Bussolati, B; Isella, C; Fontani, L; Godio, L; Cilli, M; D'Amelio, P; Isaia, G; Medico, E; Ferracini, R; Roato, I
British journal of cancer 108 2525-36 2013

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23801032 23801032

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The Power of Biomarker Analysis

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96 well Template 2 Up
96 well Template Single
MCYP2MAG-73K xPonent Procotol Template Version 3.1
MCYP2MAG-73K xPonent Procotol Template Version Magpix
MCYP2MAG-73K xPonent Standard Template Version 3.1
MCYP2MAG-73K xPonent Standard Template Version Magpix
Quality Control Ranges: Milliplex Mouse Cytokine Panel 2 Panel 2 Catalog# MCYP2MAG-73K

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