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70666 | Ni-NTA His•Bind® Resin

70666
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      Replacement Information

      Precios y disponibilidad

      Número de referenciaDisponiblidad Embalaje Cant./Env. Precio Cantidad
      US170666-3
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      Disponibilidad a confirmarDisponibilidad a confirmar
      En existencia 
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      Cantidades limitadas disponibles
      Debe confirmarse disponibilidad
        El resto: se avisará
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          Se avisará
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          Frasco de vidrio 10 ml
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          US170666-4
          Comprobando disponibilidad...
          Disponibilidad a confirmarDisponibilidad a confirmar
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          Suspendido
          Cantidades limitadas disponibles
          Debe confirmarse disponibilidad
            El resto: se avisará
              El resto: se avisará
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              Frasco de vidrio 25 ml
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              Description
              OverviewNi-NTA His•Bind® Resin is a high-performance Ni2+-charged agarose used for rapid one-step purification of proteins containing a His•Tag® sequence by metal chelation chromatography. NTA chemistry minimizes metal leaching during purification and is compatible with up to 10 mM β-mercaptoethanol or 1 mM Tris(hydroxypropyl)phosphine (THP) for reduction of disulfide bonds. Ni-NTA His•Bind® Resin has a binding capacity of 5–10 mg His•Tag® fusion protein per ml resin.

              Supplied as a 50% slurry; the quantity/pack is based on the amount of the settled resin.
              Catalogue Number70666
              Brand Family Novagen®
              References
              Product Information
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              R PhraseR: 10-22-42/43

              Flammable.
              Harmful if swallowed.
              May cause sensitization by inhalation and skin contact.
              S PhraseS: 26-36-7

              In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
              Wear suitable protective clothing.
              Keep container tightly closed.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Shipped at Ambient Temperature or with Blue Ice
              Toxicity Flammable
              Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
              Storage +2°C to +8°C
              Do not freeze Yes
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              Ficha datos de seguridad (MSDS)

              Título

              Ficha técnica de seguridad del material (MSDS) 

              Certificados de análisis

              CargoNúmero de lote
              70666

              Folleto

              Cargo
              Protein Purification and Detection Tools
              Protein purification and preparation

              Licencias necesarias e Información técnica

              Cargo
              Ni-NTA HisユBind® Resins

              Citas

              Título
            • Aipo Diao, et al. (2008) Coordination of golgin tethering and SNARE assembly: GM130 binds syntaxin 5 in a p115-regulated manner. Journal of Biological Chemistry 283, 6957-6967.
            • Jennifer L. Farmer, et al. (2008) Galectin 15 (LGALS15) functions in trophectoderm migration and attachment. Federation of American Societies for Experimental Biology Journal 22, 548-560.
            • Megan Mittelstadt, et al. (2008) Interaction of human tRNA-dihydrouridine synthase-2 with interferon-induced protein kinase PKR. Nucleic Acids Research 36, 998-1008.
            • Wei Cao, et al. (2007) RAET1E2, a soluble isoform of the UL16 binding protein RAET1E produced by tumor cells, inhibits NKG2D-mediated NK cytotoxicity.. Journal of Biological Chemistry 282, 18922-18928.
            • Buxin Chen, Gary P. Newnam and Yury O. Chernoff. (2007) Prion species barrier between the closely related yeast proteins is detected despite coaggregation. Procedings of the National Academy of Science 104, 2791-2796.
            • Sulene L. Chi, et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Research 67, 4716-4724.
            • Bei Han, et al. (2007) Molecular characterization of a glucokinase with broad hexose specificity from Bacillus sphaericus strain C3-41. Applied and Enviornmental Microbiology 73, 3581-3586.
            • Akinori Ikegami, et al. (2007) The CHLI1 subunit of Arabidopsis thaliana magnesium chelatase is a target protein of the chloroplast thioredoxin. Journal of Biological Chemistry 282, 19282-19291.
            • Hisashi Nishiwaki, et al. (2007) Cloning, functional characterization, and mode of action of a novel insecticidal pore-forming toxin, sphaericolysin, produced by Bacillus sphaericus. Applied and Enviornmental Microbiology 73, 3404-3411.
            • Ernest P. Williams, et al. (2007) Mycobacterium tuberculosis SigF regulates genes encoding cell wall-associated proteins and directly regulates the transcriptional regulatory gene phoY1. Journal of Bacteriology 189, 4234-4242.
            • Hwan Youn, et al. (2007) A C-helix residue, arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein. Journal of Biological Chemistry 282, 3632-3639.
            • Christian Zuber, et al. (2007) EDEM1 reveals a quality control vesicular transport pathway out of the endoplasmic reticulum not involving the COPII exit sites. Procedings of the National Academy of Science 104, 4407-4412.
            • Alyssa Carré-Mlouka, et al. (2006) A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis. Journal of Biological Chemistry 281, 24462-24471.
            • Gerard M. Gibbs, et al. (2006) The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. Journal of Biological Chemistry 281, 4156-4163.
            • Yannick Gueguen, et al. (2006) Characterization of a defensin from the oyster Crassostrea gigas: recombinant production, folding, solution structure, antimicrobial activities, and gene expression. Journal of Biological Chemistry 281, 313-323.
            • Jeong Hoon Kim, Catherine K. Yang and Michael R. Stallcup. (2006) Downstream signaling mechanism of the C-terminal activation domain of transcriptional coactivator CoCoA. Nucleic Acids Research 34, 2736-2750.
            • Chad E. Metcalf and David A. Wassarman. (2006) DNA binding properties of TAF1 isoforms with two AT-hooks. Journal of Biological Chemistry 281, 30015-30023.
            • Anush Oganesian, et al. (2006) The NH2-terminal propeptide of type I procollagen acts intracellularly to modulate cell function. Journal of Biological Chemistry 281, 38507-38518.
            • Buxin Chen, Gary P. Newnam and Yury O. Chernoff. (2007) Prion species barrier between the closely related yeast proteins is detected despite coaggregation. Procedings of the National Academy of Science 104, 2791-2796.
            • Sulene L. Chi, et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Research 67, 4716-4724.
            • Bei Han, et al. (2007) Molecular characterization of a glucokinase with broad hexose specificity from Bacillus sphaericus strain C3-41. Applied and Enviornmental Microbiology 73, 3581-3586.
            • Akinori Ikegami, et al. (2007) The CHLI1 subunit of Arabidopsis thaliana magnesium chelatase is a target protein of the chloroplast thioredoxin. Journal of Biological Chemistry 282, 19282-19291.
            • Hisashi Nishiwaki, et al. (2007) Cloning, functional characterization, and mode of action of a novel insecticidal pore-forming toxin, sphaericolysin, produced by Bacillus sphaericus. Applied and Enviornmental Microbiology 73, 3404-3411.
            • Ernest P. Williams, et al. (2007) Mycobacterium tuberculosis SigF regulates genes encoding cell wall-associated proteins and directly regulates the transcriptional regulatory gene phoY1. Journal of Bacteriology 189, 4234-4242.
            • Hwan Youn, et al. (2007) A C-helix residue, arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein. Journal of Biological Chemistry 282, 3632-3639.
            • Christian Zuber, et al. (2007) EDEM1 reveals a quality control vesicular transport pathway out of the endoplasmic reticulum not involving the COPII exit sites. Procedings of the National Academy of Science 104, 4407-4412.
            • Alyssa Carré-Mlouka, et al. (2006) A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis. Journal of Biological Chemistry 281, 24462-24471.
            • Gerard M. Gibbs, et al. (2006) The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. Journal of Biological Chemistry 281, 4156-4163.
            • Yannick Gueguen, et al. (2006) Characterization of a defensin from the oyster Crassostrea gigas: recombinant production, folding, solution structure, antimicrobial activities, and gene expression. Journal of Biological Chemistry 281, 313-323.
            • Jeong Hoon Kim, Catherine K. Yang and Michael R. Stallcup. (2006) Downstream signaling mechanism of the C-terminal activation domain of transcriptional coactivator CoCoA. Nucleic Acids Research 34, 2736-2750.
            • Chad E. Metcalf and David A. Wassarman. (2006) DNA binding properties of TAF1 isoforms with two AT-hooks. Journal of Biological Chemistry 281, 30015-30023.
            • Anush Oganesian, et al. (2006) The NH2-terminal propeptide of type I procollagen acts intracellularly to modulate cell function. Journal of Biological Chemistry 281, 38507-38518.
            • Ray S. Oh, Xinli Bai and Johanna M. Rommens. (2006) Human homologs of UbC6P ubiquitin-conjugating enzyme and phosphorylation of HSUbC6E in response to endoplasmic reticulum stress. Journal of Biological Chemistry 281, 21480-21490.
            • Ganna Panasyuk, et al. (2006) Nuclear export of S6K1 II is regulated by protein kinase CK2 phosphorylation at ser 17. Journal of Biological Chemistry 281, 31188-31201.
            • Lauren E. Ball, Mary N. Berkaw and Maria G. Buse. (2005) Identification of the major site of O-linked ß-N-acetylglucosamine modification in the C-terminus of insulin receptor substrate-1. Molecular and Cellular Proteomics 5, 313-323.
            • Jeffrey Bethony, et al. (2005) Antibodies against a secreted protein from hookworm larvae reduce the intensity of hookworm infection in humans and vaccinated laboratory animals. Federation of American Societies for Experimental Biology Journal 19, 1743-1745.
            • Rawshan Choudhury, et al. (2005) Lowe syndrome protein OCRL1 interacts with clathrin and regulates protein trafficking between endosomes and the trans-Golgi network. Molecular Biology of the Cell 16, 3467-3479.
            • Aki Jarvinen, et al. (2005) Metabolic stability of alpha -methylated polyamine derivatives and their use as substitutes for the natural polyamines. Journal of Biological Chemistry 280, 6595-6601.
            • Choong-Min Kang, et al. (2005) The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes and Development 19, 1692-1704.
            • Erkan Karakas, et al. (2005) Structural insights into sulfite oxidase deficiency. Journal of Biological Chemistry 280, 33506-33515.
            • Torsten Kessler, et al. (2005) Inhibition of tumor growth by RGD peptide-directed delivery of truncated tissue factor to the tumor vasculature. Clinical Cancer Research 11, 6317-6324.
            • Nadia Lamour, et al. (2005) Proline metabolism in procyclic Trypanosoma brucei is down-regulated in the presence of glucose. Journal of Biological Chemistry 280, 11902-11910.
            • Maria V. Lara, et al. (2005) NADP-malic enzyme and Hsp70: co-purification of both proteins and modification of NADP-malic enzyme properties by association with Hsp70. Plant and Cell Physiology 46, 997-1006.
            • Thomas J. Lie, Gwendolyn E. Wood and John A. Leigh. (2005) Regulation of nif expression in Methanococcus maripaludis: roles of the euryarchaeal repressor NrpR, 2-oxoglutarate, and two operators. Journal of Biological Chemistry 280, 5236-5241.
            • Ying Liu, Zhi-Xing Yao and Vassilios Papadopoulos. (2005) Cytochrome p450 17α hydroxylase/17,20 lyase (CYP17) function in cholesterol biosynthesis: identification of squalene monooxygenase (epoxidase) activity associated with CYP17 in Leydig cells. Molecular Endocrinology 19, 1918-1931.
            • Buxin Chen, Gary P. Newnam and Yury O. Chernoff. (2007) Prion species barrier between the closely related yeast proteins is detected despite coaggregation. Procedings of the National Academy of Science 104, 2791-2796.
            • Sulene L. Chi, et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Research 67, 4716-4724.
            • Bei Han, et al. (2007) Molecular characterization of a glucokinase with broad hexose specificity from Bacillus sphaericus strain C3-41. Applied and Enviornmental Microbiology 73, 3581-3586.
            • Akinori Ikegami, et al. (2007) The CHLI1 subunit of Arabidopsis thaliana magnesium chelatase is a target protein of the chloroplast thioredoxin. Journal of Biological Chemistry 282, 19282-19291.
            • Hisashi Nishiwaki, et al. (2007) Cloning, functional characterization, and mode of action of a novel insecticidal pore-forming toxin, sphaericolysin, produced by Bacillus sphaericus. Applied and Enviornmental Microbiology 73, 3404-3411.
            • Ernest P. Williams, et al. (2007) Mycobacterium tuberculosis SigF regulates genes encoding cell wall-associated proteins and directly regulates the transcriptional regulatory gene phoY1. Journal of Bacteriology 189, 4234-4242.
            • Hwan Youn, et al. (2007) A C-helix residue, arg-123, has important roles in both the active and inactive forms of the cAMP receptor protein. Journal of Biological Chemistry 282, 3632-3639.
            • Christian Zuber, et al. (2007) EDEM1 reveals a quality control vesicular transport pathway out of the endoplasmic reticulum not involving the COPII exit sites. Procedings of the National Academy of Science 104, 4407-4412.
            • Alyssa Carré-Mlouka, et al. (2006) A new rubisco-like protein coexists with a photosynthetic rubisco in the planktonic cyanobacteria Microcystis. Journal of Biological Chemistry 281, 24462-24471.
            • Gerard M. Gibbs, et al. (2006) The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. Journal of Biological Chemistry 281, 4156-4163.
            • Yannick Gueguen, et al. (2006) Characterization of a defensin from the oyster Crassostrea gigas: recombinant production, folding, solution structure, antimicrobial activities, and gene expression. Journal of Biological Chemistry 281, 313-323.
            • Jeong Hoon Kim, Catherine K. Yang and Michael R. Stallcup. (2006) Downstream signaling mechanism of the C-terminal activation domain of transcriptional coactivator CoCoA. Nucleic Acids Research 34, 2736-2750.
            • Chad E. Metcalf and David A. Wassarman. (2006) DNA binding properties of TAF1 isoforms with two AT-hooks. Journal of Biological Chemistry 281, 30015-30023.
            • Anush Oganesian, et al. (2006) The NH2-terminal propeptide of type I procollagen acts intracellularly to modulate cell function. Journal of Biological Chemistry 281, 38507-38518.
            • Ray S. Oh, Xinli Bai and Johanna M. Rommens. (2006) Human homologs of UbC6P ubiquitin-conjugating enzyme and phosphorylation of HSUbC6E in response to endoplasmic reticulum stress. Journal of Biological Chemistry 281, 21480-21490.
            • Ganna Panasyuk, et al. (2006) Nuclear export of S6K1 II is regulated by protein kinase CK2 phosphorylation at ser 17. Journal of Biological Chemistry 281, 31188-31201.
            • Lauren E. Ball, Mary N. Berkaw and Maria G. Buse. (2005) Identification of the major site of O-linked ß-N-acetylglucosamine modification in the C-terminus of insulin receptor substrate-1. Molecular and Cellular Proteomics 5, 313-323.
            • Jeffrey Bethony, et al. (2005) Antibodies against a secreted protein from hookworm larvae reduce the intensity of hookworm infection in humans and vaccinated laboratory animals. Federation of American Societies for Experimental Biology Journal 19, 1743-1745.
            • Rawshan Choudhury, et al. (2005) Lowe syndrome protein OCRL1 interacts with clathrin and regulates protein trafficking between endosomes and the trans-Golgi network. Molecular Biology of the Cell 16, 3467-3479.
            • Aki Jarvinen, et al. (2005) Metabolic stability of alpha -methylated polyamine derivatives and their use as substitutes for the natural polyamines. Journal of Biological Chemistry 280, 6595-6601.
            • Choong-Min Kang, et al. (2005) The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB: substrate identification and regulation of cell shape. Genes and Development 19, 1692-1704.
            • Erkan Karakas, et al. (2005) Structural insights into sulfite oxidase deficiency. Journal of Biological Chemistry 280, 33506-33515.
            • Torsten Kessler, et al. (2005) Inhibition of tumor growth by RGD peptide-directed delivery of truncated tissue factor to the tumor vasculature. Clinical Cancer Research 11, 6317-6324.
            • Nadia Lamour, et al. (2005) Proline metabolism in procyclic Trypanosoma brucei is down-regulated in the presence of glucose. Journal of Biological Chemistry 280, 11902-11910.
            • Maria V. Lara, et al. (2005) NADP-malic enzyme and Hsp70: co-purification of both proteins and modification of NADP-malic enzyme properties by association with Hsp70. Plant and Cell Physiology 46, 997-1006.
            • Thomas J. Lie, Gwendolyn E. Wood and John A. Leigh. (2005) Regulation of nif expression in Methanococcus maripaludis: roles of the euryarchaeal repressor NrpR, 2-oxoglutarate, and two operators. Journal of Biological Chemistry 280, 5236-5241.
            • Ying Liu, Zhi-Xing Yao and Vassilios Papadopoulos. (2005) Cytochrome p450 17α hydroxylase/17,20 lyase (CYP17) function in cholesterol biosynthesis: identification of squalene monooxygenase (epoxidase) activity associated with CYP17 in Leydig cells. Molecular Endocrinology 19, 1918-1931.
            • Kevin W. -H. Lo, et al. (2005) The 8 kDa dynein light chain binds to 53BP1 and mediates DNA damage-induced p53 nuclear accumulation. Journal of Biological Chemistry 280, 8172-8179.
            • Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189-194.
            • Seung Wook Oh, et al. (2005) NK regulates lifespan in Caenorhabditis elegans by modulating nuclear translocation of forkhead transcription factor/DAF-16. Proceedings of the National Academy of Sciences (USA) 102, 4494-4499.
            • Sabrina R. Perri, et al. (2005) Plasminogen kringle 5-engineered glioma cells block migration of tumor-associated macrophages and suppress tumor vascularization and progression. Cancer Research 65, 8359-8365.
            • Savvas Saouros, et al. (2005) A novel galectin-like domain from Toxoplasma gondii micronemal protein 1 assists the folding, assembly, and transport of a cell-adhesion complex. Journal of Biological Chemistry 280, 38583-38591.
            • D. Andrew Skaff, et al. (2005) Glucose 6-phosphate release of wild-type and mutant human brain hexokinases from mitochondria. Journal of Biological Chemistry 280, 38403-38409.
            • James A. Snipes, et al. (2005) Cloning and characterization of cyclooxygenase-1b (putative cyclooxygenase-3) in rat. Journal of Pharmacology and Experimental Therapeutics 313, 668-676.
            • Taishi Tsubouchi, et al. (2005) Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus. Journal of Biological Chemistry 280, 18511-18516.
            • Matthew W. Vetting, et al. (2005) A novel dimeric structure of the rimL N-acetyltransferase from Salmonella typhimurium. Journal of Biological Chemistry 280, 22108-22114.
            • Aileen Aherne, et al. (2004) On the molecular patholo
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              Life Science Research > Protein Sample Preparation > Protein Purification > Agarose Bead Affinity Purification > Agarose Beads for Recombinant Fusion Proteins