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17-10000 | Phosphotyrosine HTRF® Assay

17-10000
1 plate  Kit capacity: 384 wells
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      Descripción

      Replacement Information

      Tabla espec. clave

      Key ApplicationsAnalytes AvailableDetection Methods
      KA Tyrosine Kinase HTRF
      Description
      Catalogue Number17-10000
      Trade Name
      • HTRF
      DescriptionPhosphotyrosine HTRF® Assay
      OverviewThe Phosphotyrosine Detection HTRF® Assay (Cat. # 17-10000) is a complete homogenous method for measuring tyrosine kinase activities using the industry standard monoclonal phosphotyrosine antibody, 4G10®. Clone 4G10 has been validated by thousands of researchers to detect phosphorylation of virtually every tyrosine target. The kit includes Clone 4G10 conjugated to Europium, a tyrosine peptide substrate and a universal detection system, HTRF®. An increase in the HTRF® signal is observed as a result of the kinase activity. Inhibitor compounds can be screened during the kinase reaction for an IC50 value determination.
      Background InformationTyrosine Phosphorylation Protein phosphorylation is often necessary for the initiation of biological processes such as cell growth, proliferation, and ubiquitin mediated degradation. Tyrosine phosphorylation, in particular, often mediates receptor signaling events at the membrane by initiating protein-protein interactions in response to growth factors, hormones and cytokines.
      References
      Product Information
      Components
      • Black 384 well plate: (Part No. R5215) One 384-well black plate.
      • Detection buffer: (Part No. CS206478) Two bottles containing 5 mL each of detection buffer.
      • 10X KinEASE buffer: (Part No. CS206508). Two vials containing 1 mL each of kinase reaction buffer.
      • Biotinylated TK1 Peptide: (Part No. CS206504). Two vials containing 60 µL each of Biotinylated TK1 Peptide Stock at 50 µM.
      • Biotinylated TK2 Peptide: (Part No. CS206505). Two vials containing 60 µL each of Biotinylated TK2 Peptide Stock at 50 µM.
      • Biotinylated TK3 Peptide: (Part No. CS206506). Two vials containing 60 µL each of Biotinylated TK3 Peptide Stock at 50 µM.
      • Biotinylated Phosphotyrosine Peptide Standard: (Part No. 90192). One vial containing 10 µg lyophilized biotinylated phosphotyrosine peptide standard.
      • Streptavidin-APC: (Part No. CS206501). Two vials containing 360 µL each of streptavidin-APC stock at 1 µM.
      • Eu-cryptate-conjugated 4G10 anti-phosphotyrosine: (Part No. CS206507). Two vials containing 30 µL each Eu-cryptate conjugated 4G10 anti-phosphotyrosine antibody stock at 35 µg/mL.
      • ATP: (Part No. CS206509). Two vials containing 50 µL each of ATP stock at 10mM.
      • DTT: (Part No, 90499). Two vials containing 100 µL each of DTT stock at 1M.
      • MnCl2: (Part No. CS206510). Two vials containing 100 µL each of MnCl2 stock at 0.1M.
      • MgCl2: (Part No. CS206511). Two vials containing 100 µL each of MgCl2 stock at 1M.
      • Sodium Orthovanadate: (Part No. CS206465). Two vials containing 50 µL each of sodium orthovanadate stock at 50 mM.
      • Staurosporine: (Part No. CS201778). One vial containing 100 µL of staurosporine stock at 2.5 mM.
      Detection methodHTRF
      Applications
      ApplicationThe Phosphotyrosine Detection HTRF Assay is a complete homogenous method for measuring tyrosine kinase activities using the industry standard monoclonal phosphotyrosine antibody, 4G10.
      Key Applications
      • Kinase Assay
      Biological Information
      Analytes Available
      • Tyrosine Kinase
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 plate
      Material PackageKit capacity: 384 wells
      Transport Information
      Supplemental Information
      Specifications

      Productos y aplicaciones relacionados

      Accessories

      Número de referencia Descripción  
      05-321 Anti-Phosphotyrosine Antibody, clone 4G10® Precios y disponibilidad

      Productos relacionados por: Brand Facete

      Categorías

      Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays