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324355 | n-Dodecyl-β-D-maltoside, ULTROL® Grade - CAS 69227-93-6 - Calbiochem

324355
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      CAS #
      69227-93-6

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              Description
              OverviewNon-ionic detergent similar to n-octyl-β-D-glucopyranoside; however, increased alkyl chain length has been shown to increase thermal stability of lipid-free rhodopsin in solution. Useful in membrane-protein solubilization studies. Aggregation number: 98.
              Absorbance (10%, H2O, 260 nm): <1.0.
              Catalogue Number324355
              Brand Family Calbiochem®
              References
              ReferencesWitzmann, F., et al. 1991. Electrophoresis 12, 687.
              Bass, W.T., and Bricker, T.M. 1988. Anal. Biochem. 171, 330.
              Rosevear, P., et al. 1980. Biochemistry 19, 4108.
              Grip, W.J., and Bovee-Geurts, P.H.M. 1979. Chem. Phys. Lipids 23, 321.
              Stubbs, G.W., et al. 1976. Biochim. Biophys. Acta 425, 46.
              Product Information
              CAS number69227-93-6
              Aggregation Number98
              CMC100-600 µM
              FormWhite solid
              Hygroscopic Hygroscopic
              Micellar weight50000
              Structure formula Image
              Applications
              Biological Information
              Purity≥98% by HPLC
              Physicochemical Information
              Contaminantsdodecanol 1H NMR: ≤1%
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage +2°C to +8°C
              Hygroscopic Hygroscopic
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, refrigerate (4°C).
              Stock solutions are stable for up to 3 months at 4°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              Ficha datos de seguridad (MSDS)

              Título

              Ficha técnica de seguridad del material (MSDS) 

              Certificados de análisis

              CargoNúmero de lote
              324355

              Referencias bibliográficas

              Visión general referencias
              Witzmann, F., et al. 1991. Electrophoresis 12, 687.
              Bass, W.T., and Bricker, T.M. 1988. Anal. Biochem. 171, 330.
              Rosevear, P., et al. 1980. Biochemistry 19, 4108.
              Grip, W.J., and Bovee-Geurts, P.H.M. 1979. Chem. Phys. Lipids 23, 321.
              Stubbs, G.W., et al. 1976. Biochim. Biophys. Acta 425, 46.

              Folleto

              Cargo
              CALBIOSORBル Technical Bulletin

              Citas

              Título
            • Serban P. Georgescu, et al. (2005) Modulator recognition factor 1 (MRF1), an ARID-family member, is a novel co-repressor for estrogen receptor α. Molecular Endocrinology 19, 2491-2501.
            • Ficha técnica

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision20-December-2010 JSW
              DescriptionA water-soluble nonionic detergent. Studies on rhodopsin have shown that alkyl glycosides with longer alkyl chains are better at preserving the protein's properties. In addition, dodecyl maltoside's longer hydrophilic group confers higher solubility in water. This is in contrast to undecyl and dodecyl glucosides, which have low solubility in water. Due to its low CMC, dodecyl maltoside is not easily removed by dialysis. The half time for dialysis is 20 h. This detergent is milder than nonyl glucoside. Of several glycosides tested, dodecyl maltoside was found to be the most useful for the preparation of active cytochrome oxidase from Neurospora. In octyl glucoside, cytochrome oxidase dissociated into its subunits and became inactive. Similarly, beef heart oxidase when equilibrated in octyl glucoside formed a mixture of polymer, dimer, and lower molecular weight forms and underwent denaturation. In contrast, the enzyme, when equilibrated in dodecyl maltoside, was found to exist as a uniform species of 30 kDa, which is consistent with an active dimeric form. Dodecyl maltoside has a critical micelle concentration (CMC) of 100-600 µM and a micellar weight of 70,000. Aggregation number: 98.
              FormWhite solid
              CAS number69227-93-6
              Structure formula
              Purity≥98% by HPLC
              Contaminantsdodecanol 1H NMR: ≤1%
              SolubilityH₂O (100 mg/ml)
              Storage +2°C to +8°C
              Hygroscopic
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, refrigerate (4°C).
              Stock solutions are stable for up to 3 months at 4°C.
              Toxicity Standard Handling
              ReferencesWitzmann, F., et al. 1991. Electrophoresis 12, 687.
              Bass, W.T., and Bricker, T.M. 1988. Anal. Biochem. 171, 330.
              Rosevear, P., et al. 1980. Biochemistry 19, 4108.
              Grip, W.J., and Bovee-Geurts, P.H.M. 1979. Chem. Phys. Lipids 23, 321.
              Stubbs, G.W., et al. 1976. Biochim. Biophys. Acta 425, 46.
              Citation
            • Serban P. Georgescu, et al. (2005) Modulator recognition factor 1 (MRF1), an ARID-family member, is a novel co-repressor for estrogen receptor α. Molecular Endocrinology 19, 2491-2501.