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100068 | BOLTON broth (base)

100068
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      Overview

      Replacement Information

      Key Spec Table

      ph ValueSolubility
      7.2 - 7.6 (27.6 g/l, H₂O, 25 °C) (after autoclaving)27.6 g/l

      Pricing & Availability

      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      1.00068.0500
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          Plastic bottle 500 g
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          Description
          Catalogue Number100068
          OverviewGranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
          References
          Product Information
          HS Code3821 00 00
          Applications
          Biological Information
          Physicochemical Information
          pH value7.2 - 7.6 (27.6 g/l, H₂O, 25 °C) (after autoclaving)
          Solubility27.6 g/l
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Hazard Pictogram(s)
          Hazard Statement(s)H318: Causes serious eye damage.
          Precautionary Statement(s)P280: Wear eye protection.
          P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
          P313: Get medical advice/ attention.
          Storage class10 - 13 Other liquids and solids
          WGKWGK 1 slightly water endangering
          Disposal3
          Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
          Safety Information
          Hazard Symbols Irritant
          Categories of dangerirritant
          R PhraseR 36
          Irritating to eyes.
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Appearance (colour)dark red to black
          pH-value (25 °C)7.2 - 7.6
          Growth promotion test in accordance with the current version of DIN EN ISO 11133.
          Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
          Additionaly monoculture of E.coli and P.mirabilis.
          Afterwards confirmation on mCCDA resp. Tryptic soy agar.
          Inoculum on reference medium (Campylobacter coli ATCC 43478 (WDCM 00004))≤ 100
          Inoculum on reference medium (Campylobacter jejuni ATCC 33291 (WDCM 00005))≤ 100
          Inoculum on reference medium (Campylobacter jejuni ATCC 29428 (WDCM 00156))≤ 100
          Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))≥ 1000
          Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))≥ 1000
          Inoculum on reference medium (Proteus mirabilis ATCC 29906 (WDCM 00023))≥ 1000
          Growth on mCCDA (Campylobacter coli ATCC 43478 (WDCM 00004))> 10 CFU
          Growth on mCCDA (Campylobacter jejuni ATCC 33291 (WDCM 00005))> 10 CFU
          Growth on mCCDA (Campylobacter jejuni ATCC 29428 (WDCM 00156))> 10 CFU
          Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013))no growth
          Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012))no growth
          Growth on Tryptic soy agar (Proteus mirabilis ATCC 29906 (WDCM 00023))no growth
          Colony colour (Campylobacter coli ATCC 43478 (WDCM 00004))greyish
          Colony colour (Campylobacter jejuni ATCC 33291 (WDCM 00005))greyish
          Colony colour (Campylobacter jejuni ATCC 29428 (WDCM 00156))greyish
          Incubation:
          Bolton broth: 5 ± 1 hours at 37 ± 1 °C, afterwards 44 ± 4 hours at 41.5 ± 1 °C, Anaerocult C
          Confirmation on mCCDA: at least 44 ± 4 hours at 41.5 ± 1 °C, Anaerocult C
          Confirmation on Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C, aerobic

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          Accessories (Secondary)

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          Categories

          Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Product > Dehydrated and Ready-to-use Culture Media
          Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > Campylobacter
          Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media
          Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment, Isolation & Differentiation by Organism > Campylobacter