Neurite Outgrowth Assays

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Research Paper Spotlight
Merck:/Freestyle/BI-Bioscience/Antibodies-Assays/AN4463EN00_EMD-1.jpgSpatially Restricted Exposure of Toxins to Neurons and its Impact on Axonal Degeneration Using AXIS™ System.

Investigations into neurotoxic effects have been hampered by the lack of an in vitro test system capable of separating axons from their cell bodies. Using a novel microfluidic AXonal Isolation System (AXIS System) we were able to spatially isolate axons so they could be directly and specifically exposed to neurotoxins.

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Merck:/Freestyle/BI-Bioscience/Antibodies-Assays/neuroscience-images/neuro_icon11_round.gifNeurite Outgrowth is a process wherein developing neurons produce new projections as they grow in response to guidance cues. Nerve growth factors, or neurotrophins, are one family of such stimuli that regulate neurite growth. Dynamic neurite outgrowth during development results in the formation of a complex neuronal architecture that results in the establishment of the functional nervous system and brain. Understanding the biology of neurite outgrowth can shed light on mechanisms underlying certain neurodegenerative diseases.

In order to investigate neurite outgrowth, researchers need an effective methods for growing neurons in culture, stimulating the cells with neurogenic compounds, such as growth factors, and monitoring the growing neurites.

Merck understands these needs and offers innovative products for studying neurite outgrowth.
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Neurite Outgrowth Quantification Assay

Merck’s NS230 Neurite Outgrowth Assay Plus Kit (3 µm) utilizes microporous tissue culture insert technology from Merck and is based on the use of Millicell inserts which contain a permeable membrane with 3-µm pores. The Millicell inserts utilized in this kit are appropriate for cells projecting neurites of up to 3 µm.

AXIS™ Axon Investigation System:

An advanced platform for spatially controlled neurite outgrowth and analysis

Merck:/Freestyle/BI-Bioscience/Antibodies-Assays/n1e-115-cells.jpg
N1E-115 cells clearly demonstrate neurite outgrowth through the AXIS channels (150µm) using the Milli-Mark™ FluoroPan neuronal marker (MAB2300X) shown in green, versus DAPI (blue).
AXIS Axon Isolation Device is a two chamber system, each composed of two wells and an interconnected channel, separated by a set of microgrooves. The hydrostatic pressure formed by volume differential between chambers induces fluidic isolation of the solution on the low volume side of the device.

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