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613548 | TMB, Insoluble

613548
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      Precios y disponibilidad

      Número de referenciaDisponiblidad Embalaje Cant./Env. Precio Cantidad
      US1613548-100ML
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          Frasco de vidrio 100 ml
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          Description
          OverviewPeroxidase substrate that produces a stable, insoluble, aquamarine blue precipitate at reaction site with little or no background.
          Catalogue Number613548
          Brand Family Calbiochem®
          Synonyms3,3ʹ,5,5ʹ-Tetramethylbenzidine
          References
          Product Information
          CAS number54827-17-7
          FormClear to light brown liquid
          Formulation1.19 mM H₂O₂ in a proprietary solvent, 1.13 mM TMB, pH 4.9 ± 0.03.
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSDV2300000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Carcinogenic / Teratogenic
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsDiscard if the solution turns blue or turbid. The reagent is stable at room temperature; refrigeration is recommended for long-term storage. Warm to assay temperature before use.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          Certificados de análisis

          CargoNúmero de lote
          613548

          Folleto

          Cargo
          Enzyme-Linked Detection Systems Technical Bulletin
          Ficha técnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision12-September-2008 RFH
          Synonyms3,3ʹ,5,5ʹ-Tetramethylbenzidine
          DescriptionInsoluble TMB substrate supplied as a single component system. TMB is the most sensitive chromogenic substrate for detection of horseradish peroxidase (HRP)-labeled probes. In the presence of HRP and hydrogen peroxide (H2O2), TMB is oxidized first to a blue, cation free radical; upon further reaction with HRP/H2O2, or addition of acid, the radical is converted to a yellow diimine terminal oxidation product. TMB is used routinely in ELISA procedures, whereby its soluble oxidation products are measured spectrophotometrically. For TMB to be worthwhile as a blotting reagent, enhancers such as dextran sulfate or other sugar polymers are usually added.This TMB reagent is a safe, single component system that has been developed for use in blotting procedures by a proprietary methodology that eliminates the use of sugar polymers. The reagent produces a stable, insoluble aquamarine precipitate at the reaction site with little or no background. It can significantly increase the detection limits of assays on a variety of membranes. Ideal for many immunoblotting procedures and can significantly increase detection limits of assays on a variety of membranes
          FormClear to light brown liquid
          Formulation1.19 mM H₂O₂ in a proprietary solvent, 1.13 mM TMB, pH 4.9 ± 0.03.
          Recommended reaction conditionsSuggested Procedure for Immunoblot Staining with TMB: 1. Complete all required incubations with antibodies and HRP-labeled probes. 2. Wash membranes thoroughly with phosphate-buffered saline (PBS) or Tris-buffered saline (TBS), containing 0.1% Tween®-20 detergent. Note: Do not use reagents containing thimerosal or phenolic compounds as preservatives. 3. Following the final wash, cover the membranes completely with TMB solution and incubate with gentle rocking for 5-30 min, or until the desired color intensity is obtained (aquamarine/blue bands or dots will appear at the sites of enzyme activity). Note: Variables associated with assay conditions will dictate the proper reaction time. If the color develops immediately, the TMB precipitate may flake off the membrane. If this occurs, further dilution of the HRP probe is recommended. A fine blue line circumscribing the band or dot also suggests that dilution of the enzyme is necessary. 4. Stop the reaction by washing the membrane thoroughly in distilled water. DO NOT WASH WITH BUFFERED SOLUTIONS OR TAP WATER. These will cause fading. Also, DO NOT USE ACIDS TO STOP THE REACTION. Acids will turn the bands yellow. Excessive background staining indicates incomplete removal of unbound HRP from membranes. To remedy this problem, increase the wash steps or washing times. 5. Air-dry membranes and store protected from light.
          CAS number54827-17-7
          RTECSDV2300000
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsDiscard if the solution turns blue or turbid. The reagent is stable at room temperature; refrigeration is recommended for long-term storage. Warm to assay temperature before use.
          Toxicity Carcinogenic / Teratogenic