Licencias necesarias e Información técnica
|MultiScreen Solvinert and MultiScreen Deep Well Solvinert Filter Plates; 96-well chemically compatible sample prep platforms|
|Automated Sample Preparation Method for High Throughput Total Drug Analysis by LC-MS/MS|
|Automation of ADME Applications|
|Poster: Rapid Sample Preparation Method for High Throughput Total Drug Analysis by LC-MS/MS|
|Visión general referencias||Aplicación|
|LC–MS-MS Total Drug Analysis of Biological Samples Using a High-Throughput Protein Precipitation Method |
Bogdan Sleczka, Jian Wang, and Timothy Olah, Bristol-Myers Squibb Bioanalytical and Discovery Analytical Sciences, Princeton, New Jersey
LC-GC, Volume 24 Number 7 July 2006 2006
|Assessment of filter plates for multi-well in-gel digestion of proteins separated by polyacrylamide gel elelctrophoresis to identify them with LC-ESI/MSMS |
Kazuhisa Kameyama, Tomohiro Nanri, Yuko Yamanaka, Mikikio Arima, Hiroshi Kawasaki
Journal Electrophoresis. 2005; 49, 71-75 2005
|What is the maximum volume that should be used with the MultiScreen Deep Well filter plate?||The maximum recommended volume to be used with the MultiScreen Deep Well filter plate is 1.8 mL (1.5 mL with shaking).|
|What is the active membrane surface area of the MultiScreen Solvinert Deep Well plate?||The active membrane surface area of the MultiScreen Solvinert Deep Well plate is 0.28cm2.|
|Is the MultiScreen Solvinert Deep Well plate automation compatible?||Yes, the MultiScreen Solvinert Deep Well plate is automation compatible.|
|When using the MultiScreen Solvinert or Solvinert Deep Well for Total Drug Analysis, can I use only a few wells at a time?||It is recommended that all wells of the plate be filled, preferably with similar solutions and volume. If only a portion of the 96 wells are to be used, the remaining wells should be sealed with a mat-cap or sealing tape. Please see the User Guide for specific information.|
|When using the MultiScreen Solvinert Deep Well plate for Total Drug Analysis, why is the maximum recommended volume for the manual method only 1 mL total volume for a 2 mL plate?||For most efficient mixing of the sample, the volume recommended allows for vigorous mixing without spilling from the individual wells.|
|When using the MultiScreen Solvinert Deep Well plate for Total Drug Analysis, can I filter by centrifugation using any centrifuge with a carrier plate?||A deep well filter plate assembled on top of a deep well collection block is not compatible with all centrifuge rotors and plate carriers. The carrier must have at least 86 mm of clearance in order to be compatible with the deep well filter plate/Greiner receiver block assembly.|
|Can I use any plate shaker with the MultiScreen Solvinert Deep Well filter plate when running the Total Drug Analysis protocol?||Any plate shaker is compatible with the MultiScreen Solvinert Deep Well filter plate as long as it meets the following two criteria: 1. It must be able to achieve a high shaking speed to induce vigorous mixing. 2. It must have a solid platform on which to place the plate. It is important to note that some plate shakers have a foam base which has the potential to make contact with the spouts in the underdrain. In some circumstances, this contact can initiate flow through the plate.|
|When using the MultiScreen Solvinert or Solvinert Deep Well for Total Drug Analysis, can I add the serum all at once to each well? Why do I have to drip the serum in slowly for the manual procedure?||Slow addition of the serum to each well allows for maximum surface area exposure of the plasma with the acetonitrile. Subsequent mixing of the solution provides for complete dispersion of the plasma into the acetonitrile thus allowing complete protein precipitation.|
|When using the MultiScreen Solvinert or Solvinert Deep Well for Total Drug Analysis, why does the protocol require 90% acetonitrile? Can I use 100% acetonitrile?||The specific density of acetonitrile and plasma are rather different, which can lead to incomplete mixing. Prewetting of the acetonitriile with 10% water improves mixing and allows for complete integration of the plasma/serum into the acetonitrile.|
|When using the MultiScreen Solvinert or Solvinert Deep Well plate for Total Drug Analysis, why do I have to leave the vacuum on for 30 seconds after the solution has completely filtered? Won't that cause the acetonitrile to evaporate?||It is recommended to leave the vacuum on for an additional 30 seconds to ensure complete and discrete filtrate transfer into the collection plate. Although the wells have been completely evacuated, the filtrate has not completely cleared the underdrain. An additional 30 seconds of vacuum allows for the discrete transfer of the filtrate.
Vacuum filtration results in minimal evaportaion of acetonitrile. With a vacuum time of 2 minutes, only 10-20 uL of acetonitrile are lost due to evaporation.
|When using the MultiScreen Solvinert or Solvinert Deep Well plate for Total Drug Analysis, what is the maximum total volume I can use?||The maximum total volume is dependent on the designated usage of the plate.
For out of plate protein precipitation, up to 1.9 mL can be transferred to each well without shaking. The maximum volume with shaking for the device is 1.5 mL to ensure the solution does not spill out of the wells. With such volumes one must be wary of the shaker settings and monitor the liquid level.
With in-plate protein precipitation following the protocol, 1 mL is the recommended total volume for manual sample handling while1.25 mL is the maximum volume for the automated procedure.
|Should the MultiScreen Solvinert Deep Well plate be used with vacuum or centrifuge?||The MultiScreen Solvinert Deep Well plate with both vacuum and centrifuge. However, vacuum should not be used with volitile solvents (e.g., TFA).|
|At what g force should the MultiScreen Solvinert Deep Well plates be spun?||The MultiScreen Solvinert Deep Well plates should be spun at 500-3,000 x g.|
|What is the extractables level for the MultiScreen Solvinert Deep Well filter plate?||The extractables level of the MultiScreen Solvinert Deep Well plates is extremely low, making this product ideal for processing samples to be analyzed by HPLC or LC/MS/MS.|
|When I wet out the membranes in the MultiScreen Solvinert Deep Well plate they become translucent. Is this normal?||Yes, this is completely normal and expected.|
|When using the MultiScreen Solvinert or Solvinert Deep Well plate for Total Drug Analysis, how fast should I shake the plate after adding the serum/plasma and acetonitrile to each well? At what setting should my plate shaker be set?||It is recommended to shake the plate as fast as possible without spilling the solution from the wells. With the clear wells of the Solvinert Deep Well plate it is easy to see the level of the solution in the wells as it shakes. Simply turn the dial of the plate shaker up until the solution is approaching the top of the wells. It has been our experience that plate shakers can have different gauge settings, and even the same setting on different plates can have different actual rates.|
|Can I use the automated Total Drug Analysis protocol for the MultiScreen Solvinert Deep Well plate manually?||Yes, the automated protcol, or the "in-tip" method, can be employed for manual sample handling. Pipettes such as the BiohitTM Proline 1200 uL multichannel pipettor with 1200 uL pipette tips (Biohit- Helinski, Finland) which are capable of in-tip mixing as recommended in the automated protocol are required.|
|I am interested in using biological fluids other than serum/plasma for the Total Drug Analysis protocol with the MultiScreen Solvinert or Solvinert Deep Well plate. Can I use them?||Yes, other biological fluids are compatible with all assay conditions.|
|Sometimes serum droplets adhere near the top of the wells when manually adding serum dropwise to the MultiScreen Solvinert Deep Well plate for the Total Drug Analysis protocol. Is this a problem? How do I get these droplets into the acetonitrile?||Serum droplets clinging to the sides of the wells is relatively common and not a problem. By shaking the filter plate vigorously so the liquid level in the wells approaches the top, any remaining droplets near the top of the wells will be mixed with the rest of the sample. Shaking at these high speeds ensures the entire sample is thoroughly mixed.|
|Is the MultiScreen Solvinert Deep Well filter plate compatible with any deep well receiver block?||The recommended deep well receiver block is available for purchase from Greiner (catalogue number 780285). This plate is recommended in particular because of its overall nestability with the filter plate and its automation compatibility.|
Manuales del usuario
|MultiScreen Deep Well Solvinert Filter Plate User Guide|
|MultiScreen Solvinert Filter Plates User Guide|
|MultiScreen® High Volume Filter Plate and Accessories|