NS830 | GFAP ELISA Kit

NS830
1 kit  96 wells
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      Übersicht

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      GFAP M, R, H ELISA Chromogenic
      Description
      Catalogue NumberNS830
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionGFAP ELISA Kit
      OverviewBackground
      Glial Fibrillary Acidic Protein (GFAP) is a class-III intermediate filament uniquely and ubiquitously expressed as part of the structural network in astrocytic glial cells. This specific expression makes GFAP ideal as a broad astrocytic marker in vertebrates. Astrocytes play a variety of key roles in supporting, guiding, nurturing, and signaling neuronal architecture and activity in a highly interdependent manner. Current understanding of CNS structure suggests that there are at least as many glial cells as the estimated 100 billion neurons, but perhaps upwards of 100 times more. Thus,
      the health of both neurons and astrocytes is often affected in disease and trauma. GFAP is proving to be a useful biomarker in the detection of neural degeneration. Although defects in GFAP have been reported to cause certain rare conditions, such as Alexander’s disease (Brenner et al., 2001), the observed interconnected physiology of neuronal and glial cells has enabled the use of GFAP as a neurodegeneration detection gauge. This relationship is being exploited by researchers and clinicians measuring a variety of neurological issues from cancer to trauma. GFAP has been used as a biomarker in the early detection of chemically induced cancer (Capo et al., 1997) and classical glioblastomas (Jung et al., 2007). Elevated levels of GFAP have been reported in traumatic brain injury (TBI) and appear to directly correlate with the severity of injury and brain tissue oxygenation in combat casualty care research (CCR, 2008) as well as civilian accidents (Mondello et al., 2010). GFAP has also been identified as a good biomarker in studies of toxin induced acute brain injury (Liao et al., 2008), in differentiation of hemorrhagic vs. ischemic strokes (Foerch et al., 2006), as well as in cardiac arrest (Kaneko et al., 2009). Millipore now offers an ELISA assay for sensitive measurement of GFAP protein in tissue homogenates and biological fluids (serum, plasma, CSF, and serum free media) from human, mouse, and rat.

      Principals of Procedure
      This assay is a Sandwich ELISA based sequentially on: 1) capture of soluble GFAP protein to the wells of a microtiter plate coated by a pre-titered amount of anti-GFAP monoclonal antibody; 2) removal of unbound material by washing; 3) binding of biotinylated anti-GFAP monoclonal antibody to
      the previously captured GFAP protein; 4) removal of unbound material by washing; 5) binding of streptavidin conjugated horseradish peroxidase to the biotinylated antibodies; 6) removal of excess or unbound enzyme conjugates by washing; and 7) quantification of immobilized antibody-enzyme
      complexes by monitoring horseradish peroxidase activity in the presence of the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). Enzyme activity is measured spectrophotometrically by recording absorbance readings at 450 nm. Increases in absorbency are directly proportional to the amount of
      GFAP protein captured from the sample. Therefore the protein concentration of unknowns can be derived by interpolation using a standard curve generated in the same assay with reference standards of known concentrations of GFAP.
      Alternate Names
      • GFAP
      • Glial Fibrillary Acidic Protein ELISA
      Materials Required but Not Delivered1. Absorbent paper or cloth
      2. Conventional, multi-channel, or repeating pipettes
      3. Reagent reservoirs
      4. Orbital microtiter plate shaker/washer (optional)
      5. Graduated serological pipettes
      6. Microtiter plate reader capable of reading absorbency at 450 nm
      7. Graphing software for plotting data or graph paper for manual plotting of data
      8. Microfuge tubes for standard and sample dilutions
      9. Mechanical vortex
      10. Milli-Q™ Water
      References
      Product Information
      Components
      • 96-Well Anti-GFAP Coated Plate (CS206352): 1 Plate
      • 1X Assay Buffer (CS206357): 40 mL
      • 10X Wash Buffer (CS206360): 50 mL
      • GFAP Protein Standard (CS206361): 1 Vial
      • GFAP Quality Control #1 (CS206351): 1 Vial
      • GFAP Quality Control #2 (CS206475): 1 Vial
      • Biotinylated Anti-GFAP Detection Antibody (CS206356): 12 mL
      • Enzyme Solution (CS206358): 12 mL
      • TMB Solution (CS202682): 10 mL
      • Stop Solution (60193): 12 mL
      • Plate Sealers (CS200443): 10 Sheets
      Detection methodChromogenic
      Applications
      Key Applications
      • ELISA
      Biological Information
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Analytes Available
      • GFAP
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
      Gene Symbol
      • GFAP
      UniProt Number
      UniProt SummaryFUNCTION: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
      SUBCELLULAR LOCATION: Cytoplasm. Note: Associated with intermediate filaments.
      TISSUE SPECIFICITY: Expressed in cells lacking fibronectin.
      PTM: Phosphorylated by PKN1.
      SIMILARITY: Belongs to the intermediate filament family.
      Physicochemical Information
      Sensitivity
      • 1.5 ng/mL
      Standard Curve Range
      • 1.5 to 100 ng/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStorage: The GFAP ELISA kit is shipped on ice and should be stored at 2-8°C upon arrival.
      Stability: Kit components are stable for a minimum of 6 months from the date of receipt if stored and
      handled as described above.
      Packaging Information
      Material Size1 kit
      Material Package96 wells
      Transport Information
      Supplemental Information
      Specifications

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      Kategorien

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits