MABE338 | Anti-DBF4, clone 7H8.1 Antibody

MABE338
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE338
      DescriptionAnti-DBF4, clone 7H8.1 Antibody
      Alternate Names
      • Protein DBF4 homolog A
      • Activator of S phase kinase
      • Chiffon homolog A
      • DBF4-type zinc finger-containing protein 1
      Background InformationDBF4, also known as Protein DBF4 homolog A, Activator of S phase kinase, also known as Chiffon homolog A or DBF4-type zinc finger containing protein 1, and encoded by the gene DBF4/ASK/DBF4A/ZDBF1, is the regulatory subunit for the cell cycle kinase CDC7. The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. . DBF4 Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6 proteins. DBF4 is localized to the nucleus. DBF4 is expressed in expressed in many cells particularly high in testis, thymus and most cancer cells.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-DBF4 antibody, clone 7H8.1 detects level of DBF4 & has been published & validated for use in DBF4.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected DBF4 in 200 µg of human thymus tissue lysate.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human DBF4 near the C-terminus.
      EpitopeNear C-terminus
      Clone7H8.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DBF4
      • ASK
      • DBF4A
      • ZDBF1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~77 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected DBF4 in 200 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications