MAB377A5 | Anti-NeuN Antibody, clone A60, Alexa Fluor® 555 Conjugate

MAB377A5
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MICC, IHCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue NumberMAB377A5
      DescriptionAnti-NeuN Antibody, clone A60, Alexa Fluor® 555 Conjugate
      Alternate Names
      • NEUronal Nuclei
      • Neuronal Nuclei
      Background InformationNeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
      References
      Product Information
      FormatAlexaFluor®555
      Control
      • Rat E18 primary cortex cells
      PresentationPurified mouse monoclonal IgG1 conjugated to Alexa Fluor® 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Applications
      ApplicationThis Anti-NeuN Antibody, clone A60, Alexa Fluor 555 Conjugate is validated for use in ICC, IHC for the detection of NeuN.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a representative lot detected NeuN in mouse adult brain cryosections.
      Biological Information
      ImmunogenPurified cell nuclei from mouse brain.
      CloneA60
      HostMouse
      SpecificityNeuN (or Neuronal Nuclei) reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia.
      IsotypeIgG1
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteDemonstrated to react with Rat and Mouse. Unconjugated Anti-NeuN has been shown to work with multiple species including Human, Ferret, Chicken, and Avian.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • NeuN
      Purification MethodAffinity Purfied
      Molecular WeightThe unconjugated parent antibody (Cat. No. MAB377) has an observed molecular weight in the 46-48 kDa range and possibly another band at approximately 66 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in rat E18 primary cortex cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 primary cortex cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NeuN, clone A60, Alexa Fluor#174; 555 Conjugate - 2145968 2145968
      Anti-NeuN, clone A60, Alexa Fluor#174; 555 Conjugate - 2179352 2179352
      Anti-NeuN, clone A60, Alexa Fluor#174; 555 Conjugate - 2207184 2207184
      Anti-NeuN, clone A60, Alexa Fluor#174; 555 Conjugate - 2262717 2262717
      Anti-NeuN, clone A60, Alexa Fluor#174; 555 Conjugate - Q2050299 Q2050299
      Anti-NeuN, clone A60, Alexa Fluor® -2738998 2738998
      Anti-NeuN, clone A60, Alexa Fluor® -2689654 2689654
      Anti-NeuN, clone A60, Alexa Fluor® 555 Conjugate 2485979
      Anti-NeuN, clone A60, Alexa Fluor® 555 Conjugate - 2360033 2360033
      Anti-NeuN, clone A60, Alexa Fluor® 555 Conjugate - 2297297 2297297

      References

      Reference overviewPub Med ID
      Exposure of neonatal rats to alcohol has differential effects on neuroinflammation and neuronal survival in the cerebellum and hippocampus.
      Topper, LA; Baculis, BC; Valenzuela, CF
      Journal of neuroinflammation  12  160  2015

      Show Abstract
      26337952 26337952
      Differential sensitivity of prefrontal cortex and hippocampus to alcohol-induced toxicity.
      Fowler, AK; Thompson, J; Chen, L; Dagda, M; Dertien, J; Dossou, KS; Moaddel, R; Bergeson, SE; Kruman, II
      PloS one  9  e106945  2014

      Show Abstract
      25188266 25188266
      Alcohol-induced one-carbon metabolism impairment promotes dysfunction of DNA base excision repair in adult brain.
      Fowler, AK; Hewetson, A; Agrawal, RG; Dagda, M; Dagda, R; Moaddel, R; Balbo, S; Sanghvi, M; Chen, Y; Hogue, RJ; Bergeson, SE; Henderson, GI; Kruman, II
      The Journal of biological chemistry  287  43533-42  2012

      Show Abstract
      23118224 23118224

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies