ABN90P | Anti-NeuN purified Antibody

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICC, IH(P)GpAffinity PurifiedPolyclonal Antibody
      Catalogue NumberABN90P
      DescriptionAnti-NeuN purified Antibody
      Alternate Names
      • NEUronal Nuclei
      • clone A60
      • NeuN
      Background InformationABN90P is a Guinea Pig polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are some examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
      Product Information
      FormatAffinity Purified
      • Mouse E16 brain tissue lysate
      PresentationPurified guinea pig polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-NeuN purified Antibody is validated for use in Western Blotting, ICC, IHC(P) for the detection of NeuN purified.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NeuN in mouse frontal cortex tissue.

      Immunocytochemistry Analysis: A 1:1,000-2,000 dilution from a representative lot detected NeuN in rat E18 cortex cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGuinea Pig
      SpecificityThis antibody recognizes the N-terminus of NeuN.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • NeuN
      Purification MethodAntigen Affinity Purified
      Molecular Weight~48 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse E16 brain tissue lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected NeuN on 10 µg of mouse E16 brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NeuN purified - 2123666 2123666
      Anti-NeuN purified - 2445032 2445032
      Anti-NeuN purified - 2069374 2069374
      Anti-NeuN purified - 2145116 2145116
      Anti-NeuN purified - 2201953 2201953
      Anti-NeuN purified - 2272164 2272164
      Anti-NeuN purified - 2329178 2329178
      Anti-NeuN purified - Q2013755 Q2013755
      Anti-NeuN purified -2499182 2499182
      Anti-NeuN purified -2552625 2552625


      Reference overviewPub Med ID
      Diffusion tensor and volumetric magnetic resonance measures as biomarkers of brain damage in a small animal model of HIV.
      Lentz, MR; Peterson, KL; Ibrahim, WG; Lee, DE; Sarlls, J; Lizak, MJ; Maric, D; Reid, WC; Hammoud, DA
      PloS one  9  e105752  2014

      Show Abstract
      25144656 25144656


      New Products: Volume 3, 2012

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      Life Science Research > Antibodies and Assays > Primary Antibodies