ABN31 | Anti-Rhes Antibody

ABN31
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Rhesus MacaqueWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN31
      DescriptionAnti-Rhes Antibody
      Alternate Names
      • GTP-binding protein Rhes
      • Ras homolog enriched in striatum
      • Tumor endothelial marker 2
      Background InformationRas family proteins are molecular switches, which are mediated by extracellular signals and regulate intracellular signaling pathways, thereby controlling a plethora of biological functions such as cell growth, gene transcription, mRNA stability and translation, cytoskeleton organization, peptide trafficking and secrection. Ras proteins are also involved in synaptic plasticity, learning, and memory in the central nervous system.

      As a member of the RASD subfamily of the Ras-related GTP-binding protein superfamily, Rhes is predominantly expressed in the striatal region of the brain, where it reported to influence brain development and function. It is thought that the activation and expression of the Rhes gene is mediated by thyroid hormones in the brain. In humans, a lack of such hormones is known to cause cretinism. Additionally, Rhes -/- knockout mice display marked behavioral abnormalities, a gender-dependent increase in anxiety levels, and a clear deficit in motor coordination.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human pancreas tissue lysate.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Rhes Antibody detects level of Rhes & has been published & validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:500 dilution of this antibody detected Rhes in paraffin-embeded human pancreas cells.

      Western Blot Analysis: A representative lot was used by an independent laboratory in HeLa cell extracts, and in mouse striatum protein extracts.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Rhes.
      EpitopeC-terminus.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the C-terminus of Rhes.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with Human, Mouse, Rat. Predicted on Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a Ras-related protein that is enriched in striatum. The product of this gene binds to GTP and possesses intrinsic GTPase activity. The gene belongs to the Ras superfamily of small GTPases. The exact function of this gene is unknown, but most striatum-specific mRNAs characterized to date encode components of signal transduction cascades.
      Gene Symbol
      • RASD2
      • TEM2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: GTPase signaling protein that binds to and hydrolyzes GTP. Regulates signaling pathways involving G-proteins-coupled receptor and heterotrimeric proteins such as GNB1, GNB2 and GNB3. May be involved in selected striatal competencies, mainly locomotor activity and motor coordination.

      SUBUNIT STRUCTURE: Monomer Potential. Interacts with PIK3CA and UBE2I By similarity. Interacts with GNB1, GNB2 and GNB3. Interacts with HTT; interacts with mutant HTT (mHTT) with a much higher affinity than wild type HTT.

      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor.
      Molecular Weight~33 kDa observed. UniProtKB/Swiss-Prot Q96D21 (RHES_HUMAN) lists a molecular weight of 30 kDa. A molecular weight of ~50 kDa possibly due to transcriptional variation or post-translational modification may be observed in some cell lysates (Spano, D., et al. (2004). Mol Cell Biol. 24(13):5788-5796.).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human pancreas tissue lysate.

      Western Blot Analysis: A 1:500 dilution of this antibody detected Rhes in human pancreas tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications