07-428-I | Anti-phospho eNOS Antibody (Ser1177)

07-428-I
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-428-I
      Replaces07-428
      DescriptionAnti-phospho eNOS Antibody (Ser1177)
      Alternate Names
      • Nitric oxide synthase, endothelial
      • Constitutive NOS
      • cNOS
      • EC-NOS
      • Endothelial NOS
      • eNOS
      • NOS type III
      • NOSIII
      Background InformationNO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho eNOS Antibody (Ser1177) is a highly specific rabbit polyclonal antibody, that targets eNOS/NOS III & has been tested in western blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a region near the C-terminus of human eNOS phosphorylated at Ser1177.
      EpitopeNear C-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Canine, Sheep, and Porcine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS3
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~133 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in untreated and VEGF treated HUVEC cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected phospho eNOS (Ser1177) in 10 µg of VEGF treated HUVEC cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho eNOS (Ser1177) - Q2343003 Q2343003
      Anti-phospho eNOS (Ser1177) -2544328 2544328
      Anti-phospho eNOS (Ser1177) -2623661 2623661
      Anti-phospho eNOS (Ser1177) -2637135 2637135
      Anti-phospho eNOS (Ser1177) -2646489 2646489
      Anti-phospho eNOS (Ser1177) -2691444 2691444
      Anti-phospho eNOS (Ser1177) -2702114 2702114
      Anti-phospho eNOS (Ser1177) -2733305 2733305
      Anti-phospho eNOS (Ser1177) Polyclonal Antibody 2901593

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies