AB5731 Anti-Nanog Antibody, NT

AB5731
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5731
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nanog Antibody, NT
      Alternate Names
      • Homeobox transcription factor Nanog
      • Nanog homeobox
      Background InformationNANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
      NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Whole cell lysate from mouse embryonic stem cells.
      PresentationPurified rabbit polyclonal immunoglobulin in buffer containing liquid 0.1% sodium azide.
      Applications
      ApplicationDetect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blotting:
      0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide from mouse Nanog.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAntibody recognizes Nanog.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FLJ12581
      • FLJ40451
      • hNanog
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFunction: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation By similarity.

      Subunit structure: Interacts with SMAD1 and SALL4 By similarity.

      Subcellular location: Nucleus. Ref.10

      Tissue specificity: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Ref.1 Ref.2 Ref.3 Ref.5 Ref.8

      Developmental stage: Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. Ref.10 Ref.3 Ref.8 Ref.6 Ref.9

      Miscellaneous: Exists an other tandem duplicated non-processed pseudogene (NANOGP1) and 10 other NANOG-related nucleotide sequences located on different chromosomes, all of which are processed pseudogenes lacking introns (NANOGP2 to NANOGP11); except NANOGP8 which is a retrogene.

      Sequence similarities Belongs to the Nanog homeobox family.

      Contains 1 homeobox DNA-binding domain.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Molecular Weight39 kDa (MW 34 kDa)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Embryonic Mouse Brain lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected NANOG on 10 μg of Embryonic Mouse Brain lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Nanog Antibody, NT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nanog Antibody, NT Certificates of Analysis

      TitleLot Number
      Anti-Nanog, N-terminus - 24468552446855
      Anti-Nanog, N-terminus - 19769201976920
      Anti-Nanog, N-terminus - 21530312153031
      Anti-Nanog, N-terminus - 23225292322529
      Anti-Nanog, N-terminus - 24940382494038
      Anti-Nanog, N-terminus - 31624383162438
      Anti-Nanog, N-terminus - LV1616760LV1616760
      Anti-Nanog, N-terminus - LV1679491LV1679491
      Anti-Nanog, N-terminus - NG1842891NG1842891
      Anti-Nanog, N-terminus - NG1849443NG1849443

      References

      Reference overviewApplicationSpeciesPub Med ID
      Generation of Naïve Bovine Induced Pluripotent Stem Cells Using PiggyBac Transposition of Doxycycline-Inducible Transcription Factors.
      Kawaguchi, T; Tsukiyama, T; Kimura, K; Matsuyama, S; Minami, N; Yamada, M; Imai, H
      PloS one  10  e0135403  2015

      Show Abstract
      26287611 26287611
      Nanog1 in NTERA-2 and recombinant NanogP8 from somatic cancer cells adopt multiple protein conformations and migrate at multiple M.W species.
      Liu, B; Badeaux, MD; Choy, G; Chandra, D; Shen, I; Jeter, CR; Rycaj, K; Lee, CF; Person, MD; Liu, C; Chen, Y; Shen, J; Jung, SY; Qin, J; Tang, DG
      PloS one  9  e90615  2014

      Show Abstract
      24598770 24598770
      Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells.
      Hagiwara, K; Obayashi, T; Sakayori, N; Yamanishi, E; Hayashi, R; Osumi, N; Nakazawa, T; Nishida, K
      PloS one  9  e84072  2014

      Show Abstract
      24465393 24465393
      Somatostatin receptor type 2 contributes to the self-renewal of murine embryonic stem cells.
      Xu, XX; Zhang, LH; Xie, X
      Acta pharmacologica Sinica  35  1023-30  2014

      Show Abstract
      24998255 24998255
      Role of distinct surfaces of the G9a ankyrin repeat domain in histone and DNA methylation during embryonic stem cell self-renewal and differentiation.
      Bittencourt, D; Lee, BH; Gao, L; Gerke, DS; Stallcup, MR
      Epigenetics & chromatin  7  27  2014

      Show Abstract
      25478012 25478012
      Sphingosine Phosphate Lyase Regulates Murine Embryonic Stem Cell Proliferation and Pluripotency through an S1P2/STAT3 Signaling Pathway.
      Smith, GS; Kumar, A; Saba, JD
      Biomolecules  3  351-368  2013

      Show Abstract
      24619572 24619572
      Proliferation rate of somatic cells affects reprogramming efficiency.
      Xu, Y; Wei, X; Wang, M; Zhang, R; Fu, Y; Xing, M; Hua, Q; Xie, X
      The Journal of biological chemistry  288  9767-78  2013

      Show Abstract
      23439651 23439651
      Wnt signaling regulates the lineage differentiation potential of mouse embryonic stem cells through Tcf3 down-regulation.
      Atlasi, Y; Noori, R; Gaspar, C; Franken, P; Sacchetti, A; Rafati, H; Mahmoudi, T; Decraene, C; Calin, GA; Merrill, BJ; Fodde, R
      PLoS genetics  9  e1003424  2013

      Show Abstract
      23658527 23658527
      Stress-mediated p38 activation promotes somatic cell reprogramming.
      Xu, X; Wang, Q; Long, Y; Zhang, R; Wei, X; Xing, M; Gu, H; Xie, X
      Cell research  23  131-41  2013

      Show Abstract
      23044805 23044805
      Enhancer transcribed RNAs arise from hypomethylated, Tet-occupied genomic regions.
      Pulakanti, K; Pinello, L; Stelloh, C; Blinka, S; Allred, J; Milanovich, S; Kiblawi, S; Peterson, J; Wang, A; Yuan, GC; Rao, S
      Epigenetics  8  1303-20  2013

      Show Abstract
      24135681 24135681

      Brochure

      Title
      Human Stem Cell Systems

      Data Sheet

      Title
      Anti-Nanog, N-terminus - Data Sheet
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies