16-663 Magna ChIP™ Protein A+G Magnetic Beads

16-663
50 reactions  
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      Overview

      Replacement Information

      Special Offers

      Achieve first time ChIP success with EZ-ChIP Assays
      Description
      Catalogue Number16-663
      Trade Name
      • Magna ChIP
      DescriptionMagna ChIP™ Protein A+G Magnetic Beads
      OverviewRecombinant protein A plus recombinant protein G covalently bound to magnetic beads. This magnetic A+G blend allows for the use of a wider range of antibodies than A or G alone and provides a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitation (ChIP) assays as well as RNA immunoprecipitation (RIP) assays. Compared with conventional agarose beads, magnetic beads significantly reduce handling times and mechanical stress on target immunocomplexes.
      Alternate Names
      • ChIP magnetic beads
      • ChIP magnetic A/G beads
      • ChIP magnetic A+G beads
      Background InformationChromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. Experiments comparing protein A vs. protein G vs. protein A/G magnetic bead blends revealed that a mixture of protein A and G beads worked well with a wide variety of antibody isotypes. The use of Protein A/G bead blends eliminated the need to consider which beads or kit to use in order match a particular antibody/bead binding affinity combination. In addition to simplifying the procedure, comparing the use of either protein A or protein G alone, protein A/G magnetic bead blends improved signal-to-noise ratios without decreasing the recovery of input chromatin in ChIP assays.
      References
      Product Information
      PresentationLiquid suspension. Supplied as magnetic bead slurry in phosphate buffered saline, pH 7.4, containing 0.01% Tween®-20 and 0.09% sodium azide.
      Applications
      ApplicationThis blend of protein A+G magnetic beads allows for the use of a wider range of antibodies than A or G alone & provides a rapid, reproducible & efficient collection of immunocomplexes for chromatin immunoprecipitations (ChIP) and RNA immunoprecipitations (RIP) assays
      Application NotesUse 20 µL of bead suspension per ChIP application. Includes sufficient reagents for 50 precipitation reactions. Disperse beads thoroughly before pipetting by rapid vortex.
      Biological Information
      Analytes Available
      • Protein A+G
      Physicochemical Information
      Dimensions
      Particle Size~3 µm
      Volume1 mL
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Chromatin immunoprecipitation (ChIP) using HeLa nuclear extracts and the Magna ChIP™ A Kit (Cat. #17-610).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of shipment. Do Not Freeze.
      Storage Temperature2 to 8 °C (Do not freeze)
      Packaging Information
      Material Size50 reactions
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Magna ChIP Protein A+G Magnetic Beads - 21105782110578
      Magna ChIP Protein A+G Magnetic Beads - 21471102147110
      Magna ChIP Protein A+G Magnetic Beads - 19645331964533
      Magna ChIP Protein A+G Magnetic Beads - 20008312000831
      Magna ChIP Protein A+G Magnetic Beads - 20409192040919
      Magna ChIP Protein A+G Magnetic Beads - 20614592061459
      Magna ChIP Protein A+G Magnetic Beads - 20917742091774
      Magna ChIP Protein A+G Magnetic Beads - 21920752192075
      Magna ChIP Protein A+G Magnetic Beads - 22072452207245
      Magna ChIP Protein A+G Magnetic Beads - 22468272246827

      References

      Reference overviewPub Med ID
      PGC-1β regulates mouse carnitine-acylcarnitine translocase through estrogen-related receptor α.
      Mar Gacias,Albert Pérez-Martí,Magdalena Pujol-Vidal,Pedro F Marrero,Diego Haro,Joana Relat
      Biochemical and biophysical research communications  423  2012

      Show Abstract
      22713466 22713466

      Brochure

      Title
      New Products: Volume 3, 2012

      Technical Info

      Title
      Getting started with ChIP-seq: experimental design to data analysis
      Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics