110116 | β1-2,3,4,6-N-Acetylglucosaminidase, Streptococcus pneumoniae, Recombinant, E. coli

110116
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      Precios y disponibilidad

      Número de referenciaDisponiblidad Embalaje Cant./Env. Precio Cantidad
      110116-1U
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      Disponibilidad a confirmarDisponibilidad a confirmar
      En existencia 
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      Debe confirmarse disponibilidad
        El resto: se avisará
          El resto: se avisará
          Se avisará
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          Description
          OverviewRecombinant, Streptococcus pneumoniae β1-2,3,4,6-N-Acetylglucosaminidase expressed in E. coli. Catalyzes the hydrolysis of β1-2,3,4,6-linked N-acetylglucosamine from complex carbohydrates. Not known to cleave substituted or branched hexosamines. Does not cleave N-acetylgalactosamine residues.
          Catalogue Number110116
          Brand Family Calbiochem®
          SynonymsN-Acetyl-β-D-glycosaminide-N-acetylglucosaminohydrolase
          References
          ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
          Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
          Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
          Product Information
          CAS number9012-33-3
          Activity≥ 35 units/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol <i>p</i>-nitrophenol from p-nitrophenyl-β-D-N-acetylglucosaminide per minute at 37°C, pH 5.0.
          EC number3.2.1.30
          FormLiquid
          FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
          Applications
          Biological Information
          Specific Activity≥ 80 units/mg protein
          Physicochemical Information
          ContaminantsEndo-α-N-acetylgalactosaminidase, α- and β-galactosidase, neuraminidases, proteases: none detected.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          Certificados de análisis

          CargoNúmero de lote
          110116

          Referencias bibliográficas

          Visión general referencias
          Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
          Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
          Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
          Ficha técnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-April-2008 RFH
          SynonymsN-Acetyl-β-D-glycosaminide-N-acetylglucosaminohydrolase
          DescriptionRecombinant, Streptococcus pneumoniae β1-2,3,4,6-N-Acetylglucosaminidase expressed in E. coli. Catalyzes the hydrolysis of β1-2,3,4,6-linked N-acetylglucosamine from complex carbohydrates. Not known to cleave substituted or branched hexosamines. Does not cleave N-acetylgalactosamine residues.
          FormLiquid
          FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
          Recommended reaction conditions50 mM sodium phosphate buffer, pH 5.0.
          CAS number9012-33-3
          EC number3.2.1.30
          ContaminantsEndo-α-N-acetylgalactosaminidase, α- and β-galactosidase, neuraminidases, proteases: none detected.
          Specific activity≥ 80 units/mg protein
          Activity≥ 35 units/ml
          Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-N-acetylglucosaminide per minute at 37°C, pH 5.0.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
          Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
          Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.