Kit de 96 pocillos para preparación de plásmidos y BAC

Simply clear, concentrate, wash, and recover

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Kit de 96 pocillos para preparación de plásmidos y BAC Borrar Clasificación y Filtrado
Número de referenciaicon Descripciónicon Tamaño de envaseicon
LSKP09604Kit Plasmid Miniprep96 4 Precios y disponibilidad
LSKP09624Kit Plasmid Miniprep96 24 Precios y disponibilidad

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Accesorios Borrar Clasificación y Filtrado
Número de referenciaicon Descripciónicon Tamaño de envaseicon
SCGVU01REUnidad de filtración Stericup-GV; 0,22 µm, PVDF, 150 ml, esterilizada por radiación 12 Precios y disponibilidad

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Equipo necesario Borrar Clasificación y Filtrado
Número de referenciaicon Descripciónicon Tamaño de envaseicon
MSVMHTS00Colector de vacío MultiScreenHTS 1 Precios y disponibilidad
WP6110060Bomba de servicio químico, 100 V/50–60 Hz 1 Precios y disponibilidad
WP6111560Bomba de servicio químico, 115 V/60 Hz 1 Precios y disponibilidad
WP6122050Bomba de servicio químico, 220 V/50 Hz 1 Precios y disponibilidad

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Reactivos* Borrar Clasificación y Filtrado
Número de referenciaicon Descripciónicon Volumenicon
LSKCLS500Solución de lisis celular; 0,5 l 500 mL Precios y disponibilidad
LSKCRS500Solución para resuspensión celular; 0,5 l 500 mL Precios y disponibilidad
LSKCTB500Tampón Tris, 0,5 l 500 mL Precios y disponibilidad
LSKNF0500Agua libre de nucleasas; 0,5 l 500 mL Precios y disponibilidad
LSKNS0500Solución neutralizadora; 0,5 l 500 mL Precios y disponibilidad
LSKPMRN30RNasa A; 0,9 ml 0.9 mL Precios y disponibilidad

*Todos los reactivos se suministran en 1 frasco/paquete

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    Documentation

    Licencias necesarias e Información técnica

    Cargo
    Fully Automated Method for Plasmid Mini-Preps Using Montage Plasmid Miniprep96 Kits on the Biomek® 2000
    Plasmid Minipreparation using MultiScreen 96-Well Filter Plates
    Tips to Enhance Operation of the Montage BAC96 Kit

    Ficha técnica

    Cargo
    DNA Template Prep and Sequencing Reaction Cleanup
    Montage Plasmid Miniprep96 Kit

    Preguntas frecuentes

    PreguntaRespuesta
    Can I use this kit in centrifugal mode?Use of the Montage Plasmid Miniprep-96 Kit in a centrifugal protocol is not recommended.
    I have observed RNA contamination of my plasmid preps. Why?Failure to add RNase A to solution 1 will often result in RNA contamination in your DNA miniprep.
    I have observed that the wells of the MultiScreen plate used in the Montage kit are not filtering uniformly. Why?The wells of the MultiScreen plates usually filter uniformly. However, a bubble in the lysate may cause an airlock, which halts filtration. Also, a highly viscous sample may increase filtration time considerably.
    When using the Montage kits how can I tell when filtration in the MultiScreen plate is complete?Filtration is complete when the individual wells of the MultiScreen plate are empty and the membranes appear shiny.
    What is holdup volume of a MultiScreen Plate used with the Montage Kits?The holdup volume for the MultiScreen plate is between 5-20 microliters.
    The two sections of the MultiScreen plates used in the Montage kits are separating under vacuum. Why?The most common reason for this problem is the lack of a MultiScreen underdrain support grid in the vacuum manifold. A MultiScreen support grid is required for proper functioning of the MultiScreen plate with any vacuum manifold.
    What is the total processing time for plasmid preparation using the Montage kits?Processing time depends on viscosity of sample, number of manifolds, familiarity with protocol, temperature. (Also, need vacuum capable of 24 in Hg or 812.7 millibar, 609.6 torr.)
    Can the culture block in the Montage 96 Plasmid prep be autoclaved?Yes.
    The total yields with the Montage Plasmid Miniprep-96 kit seems to be less then with competitive products. Why?The Montage kit uses less culture volume (1ml vs. 1.3 ml) than most competitive kits. However the relative yield is equivalent.
    What is the 260/280 assessment ratio when using the Montage Plasmid Kit?The 260/280 reading is 1.8-2.0. This signifies and extremely high level of purity.

    Manuales del usuario

    Cargo
    Beckman Compatible Base Kit for MultiScreen™ Solvent Resistant Vacuum Manifold
    Montage Plasmid Miniprep96 Kit Laminated Card
    Montage Plasmid MiniprepHTS Kit User Guide
    MultiScreen HTS PLASMID 96-Well Plates User Guide
    MultiScreen Plasmid 384 Miniprep Clearing Plate