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181315 ARE Activator, BTZO-1 - Calbiochem

181315
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Empirical Formula
C₁₃H₈N₂OS

Products

Número de referenciaEmbalaje Cant./Env.
181315-10MG Frasco de vidrio 10 mg
Description
OverviewA cell-permeable benzothiazinone that is shown to interact with MIF homotrimer (Kd = 68.6 nM and 157 nM toward human and rat MIF, respectively, at pH 7.3) via the N-terminal proline in a pH-dependent manner, limiting its inhibitory capability against MIF tautomerase activity (13% inhibition at pH 6.0; [BTZO-1] = 30 µM) due to reduced MIF binding at acidic pH. Shown to protect primary rat cardiomyocytes (330 nM) from apoptotic death upon serum deprivation or DOX (200 nM; Cat. No. 324380) treatment via activation of ARE- (antioxidant response element) mediated cytoprotective genes transcription in a MIF-dependent manner.
Catalogue Number181315
Brand Family Calbiochem®
SynonymsMacrophage Migration Inhibitory Factor Antagonist II, MIF Antagonist II, 2-Pyridin-2-yl-4H-1,3-benzothiazin-4-one, Antioxidant Response Element Activator
References
ReferencesKimura, H., et al. 2010. Chem. Biol. 17, 1282.
Product Information
FormOff-white solid
Hill FormulaC₁₃H₈N₂OS
Chemical formulaC₁₃H₈N₂OS
Structure formula ImageStructure formula Image
Applications
Biological Information
Purity≥98% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Regulatory Review
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freez e(-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

ARE Activator, BTZO-1 - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Referencias bibliográficas

Visión general referencias
Kimura, H., et al. 2010. Chem. Biol. 17, 1282.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-July-2012 JSW
SynonymsMacrophage Migration Inhibitory Factor Antagonist II, MIF Antagonist II, 2-Pyridin-2-yl-4H-1,3-benzothiazin-4-one, Antioxidant Response Element Activator
DescriptionA cell-permeable benzothiazinone that is shown to interact with MIF homotrimer (Kd = 68.6 nM and 157 nM toward human and rat MIF, respectively, at pH 7.3) via the N-terminal proline of each subunit in a pH-dependent manner, limiting its inhibitory capability against MIF tautomerase activity at acidic pH (13% inhibition at pH 6.0; [BTZO-1] = 30 µM) due to reduced MIF binding at acidic pH. Shown to prevent primary rat cardiomyocyte apoptotic death upon serum deprivation (2.38-fold of no-drug control survival rate after 4 days; [BTZO-1] = 330 nM) or DOX (200 nM; Cat. No. 324380) treatment (95% vs. 40% viability after 18 h, respectively, with or without 330 nM BTZO-1) via activation of ARE- (antioxidant response element) mediated cytoprotective genes transcription (5.3- and 3.6-fold, respectively, of control STS Ya and HO-1 mRNA after 24 h; [BTZO-1] = 1 µM) in a MIF-dependent manner.
FormOff-white solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₃H₈N₂OS
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (50 mg/ml)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freez e(-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Regulatory Review
ReferencesKimura, H., et al. 2010. Chem. Biol. 17, 1282.