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346101 Androgen Receptor, His•Tag®, Human, Recombinant, S. frugiperda

346101
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
346101-5000U Ampolla de plást. 5000 u
Description
OverviewRecombinant, human androgen receptor (AR) fused at the N-terminus to a Flag-Tag™ sequence and at the C-terminus to His•Tag® sequence, expressed in S. frugiperda insect cells expressed using a baculovirus expression system, and purified by affinity chromatography. The activity of androgen is mediated by the binding of the intracellular androgen receptor which, when activated, translocates to the nucleus and modulates specific gene transcription by interaction with cis-acting hormone response elements in the target genes.
Catalogue Number346101
Brand Family Calbiochem®
SynonymsAR, Human, Recombinant, Dihydrotestosterone Receptor, Human, Recombinant
References
ReferencesChen, G., et al. 2005. Prostate 63, 395.
Estebanez-Perpina, E., et al. 2005, J. Biol. Chem. 280, 8060.
Gao, S., et al. 2005. Mol. Endocrinol. 19, 1792.
Hillmer, A.M., et al. 2005. Am. J. Hum. Genet. 77, 140.
Kinoshita, M., et al. 2005, Int. J. Urol. 12, 390.
Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 5156;
Marques, R.B., et al. 2005. Int. J. Cancer 117, 221;
Metzger, E., et al. 2005. Nature 437, 436;
Murthy, S., et al. 2005. Prostate 64, 362;
Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5946.
Product Information
Activity1.0 unit is equivalent to 1 ng protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.
Unit of DefinitionOne unit is defined as 1 ng purified protein.
FormLiquid
FormulationIn 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 8.0.
Applications
Biological Information
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Androgen Receptor, His•Tag®, Human, Recombinant, S. frugiperda Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Androgen Receptor, His•Tag®, Human, Recombinant, S. frugiperda Certificados de análisis

CargoNúmero de lote
346101

Referencias bibliográficas

Visión general referencias
Chen, G., et al. 2005. Prostate 63, 395.
Estebanez-Perpina, E., et al. 2005, J. Biol. Chem. 280, 8060.
Gao, S., et al. 2005. Mol. Endocrinol. 19, 1792.
Hillmer, A.M., et al. 2005. Am. J. Hum. Genet. 77, 140.
Kinoshita, M., et al. 2005, Int. J. Urol. 12, 390.
Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 5156;
Marques, R.B., et al. 2005. Int. J. Cancer 117, 221;
Metzger, E., et al. 2005. Nature 437, 436;
Murthy, S., et al. 2005. Prostate 64, 362;
Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5946.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-November-2008 JSW
SynonymsAR, Human, Recombinant, Dihydrotestosterone Receptor, Human, Recombinant
DescriptionRecombinant, human androgen receptor (AR) fused at the N-terminus to a Flag-Tag™ sequence and at the C-terminus to His•Tag® sequence, expressed in S. frugiperda insect cells expressed using a baculovirus expression system, and purified by affinity chromatography. The activity of androgen is mediated by the binding of the intracellular androgen receptor which, when activated, translocates to the nucleus and modulates specific gene transcription by interaction with cis-acting hormone response elements in the target genes.
FormLiquid
FormulationIn 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Activity1.0 unit is equivalent to 1 ng protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.
Unit definitionOne unit is defined as 1 ng purified protein.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesChen, G., et al. 2005. Prostate 63, 395.
Estebanez-Perpina, E., et al. 2005, J. Biol. Chem. 280, 8060.
Gao, S., et al. 2005. Mol. Endocrinol. 19, 1792.
Hillmer, A.M., et al. 2005. Am. J. Hum. Genet. 77, 140.
Kinoshita, M., et al. 2005, Int. J. Urol. 12, 390.
Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 5156;
Marques, R.B., et al. 2005. Int. J. Cancer 117, 221;
Metzger, E., et al. 2005. Nature 437, 436;
Murthy, S., et al. 2005. Prostate 64, 362;
Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5946.