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176871 Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells

176871
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Products

Número de referenciaEmbalaje Cant./Env.
176871-10UG Ampolla de plást. 10 μg
Description
OverviewRecombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. Inactivates bradykinin by the sequential removal of two C-terminal dipeptides. ACE is a type I membrane protein that exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular mass of the glycosylated protein appears larger than 120 kDa under either reducing or non-reducing condition.
Catalogue Number176871
Brand Family Calbiochem®
SynonymsPeptidyl-Dipetidase A, ACE
References
ReferencesWakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci. 977, 232.
Hu, J., et al. 2001. J. Biol. Chem. 276, 47863.
Kessler, S.P., et al. 2000. J. Biol. Chem. 275, 26259.
Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA. 85, 9386.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
FormLiquid
FormulationIn 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl₂, 40% glycerol, pH 7.5.
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥1 unit/µg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsEndotoxins: ≤1.0 EU/µg ACE
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells Certificados de análisis

CargoNúmero de lote
176871

Referencias bibliográficas

Visión general referencias
Wakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci. 977, 232.
Hu, J., et al. 2001. J. Biol. Chem. 276, 47863.
Kessler, S.P., et al. 2000. J. Biol. Chem. 275, 26259.
Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA. 85, 9386.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-April-2008 RFH
SynonymsPeptidyl-Dipetidase A, ACE
DescriptionRecombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. ACE gene polymorphism has been a recent topic in Alzheimer's disease studies. ACE is a type I membrane protein and exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular weight of the glycosylated protein appears larger to be than 120 kDa under both reducing and non-reducing conditions.
FormLiquid
FormulationIn 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl₂, 40% glycerol, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
ContaminantsEndotoxins: ≤1.0 EU/µg ACE
Specific activity≥1 unit/µg protein
Unit definitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesWakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci. 977, 232.
Hu, J., et al. 2001. J. Biol. Chem. 276, 47863.
Kessler, S.P., et al. 2000. J. Biol. Chem. 275, 26259.
Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA. 85, 9386.