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176905 Anthrax Protective Antigen, Bacillus anthracis, Recombinant, B. anthracis - Calbiochem

176905
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Products

Número de referenciaEmbalaje Cant./Env.
176905-100UG Ampolla de plást. 100 μg
Description
OverviewRecombinant, Bacillus anthracis anthrax protective antigen expressed in a specialized strain of B. anthracis. One of the three protein components of Anthrax toxin, protective antigen (PA) is the central moiety that mediates the entry of lethal factor and edema factor into the target cell. PA binds to the cell surface via a type I membrane protein with a von Willebrand factor A domain called anthrax toxin receptor. After proteolytic activation to a 63 kDa fragment, PA self-associates to form a heptameric cation-selective, voltage-gated membrane channel.
Catalogue Number176905
Brand Family Calbiochem®
References
ReferencesMogridge, J., et al. 2002. Biochemistry 41, 1079.
Nassi, S., et al. 2002. Biochemistry 41, 1445.
Ahuja, N., et al. 2001. Biochem. Biophys. Res. Commun. 286, 6.
Bradley, K.A., et al. 2001. Nature 414, 225.
Brossier, F., and Mock, M. 2001. Toxicon 39, 1747.
Product Information
ATP CompetitiveN
FormLyophilized
FormulationLyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.
ReversibleN
Applications
ApplicationAnthrax Protective Antigen, Bacillus anthracis, Recombinant, is the protective antigen that mediates the entry of anthrax lethal factor and edema factor into the target cell.
Biological Information
Primary TargetType I membrane protein with a von Willebrand factor A domain called anthrax toxin receptor
PuritySingle major band by SDS-PAGE. Several smaller minor components are apparent that may represent cleavage fragments of the PA.
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.
End use certificateY
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anthrax Protective Antigen, Bacillus anthracis, Recombinant, B. anthracis - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anthrax Protective Antigen, Bacillus anthracis, Recombinant, B. anthracis - Calbiochem Certificados de análisis

CargoNúmero de lote
176905

Referencias bibliográficas

Visión general referencias
Mogridge, J., et al. 2002. Biochemistry 41, 1079.
Nassi, S., et al. 2002. Biochemistry 41, 1445.
Ahuja, N., et al. 2001. Biochem. Biophys. Res. Commun. 286, 6.
Bradley, K.A., et al. 2001. Nature 414, 225.
Brossier, F., and Mock, M. 2001. Toxicon 39, 1747.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-April-2008 RFH
DescriptionRecombinant, Bacillus anthracis anthrax protective antigen expressed in a special strain of B. anthracis. One of three protein components of anthrax toxin produced by the pathogenic bacterium, Bacillus anthracis. Central moiety that mediates entry of lethal factor (LF) and edema factor (EF) into the target cell. PA83 binds to the cell surface via a type I membrane receptor with a von Willebrand factor A domain, known as anthrax toxin receptor. PA83 is proteolytically activated in vivo by a furin-like protease to produce a 63 kDa protein (PA63). PA63 self-associates to form a heptameric, cation-selective, voltage-gated, membrane channel that subsequently binds to LF and/or EF. Cleavage of the 20 kDa fragment from PA83 is necessary for binding of protective antigen to LF and EF.
FormLyophilized
FormulationLyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.
PuritySingle major band by SDS-PAGE. Several smaller minor components are apparent that may represent cleavage fragments of the PA.
SolubilityReconstitute in 100 µl sterile, distilled H₂O to yield a final concentration of 1 mg/ml.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesMogridge, J., et al. 2002. Biochemistry 41, 1079.
Nassi, S., et al. 2002. Biochemistry 41, 1445.
Ahuja, N., et al. 2001. Biochem. Biophys. Res. Commun. 286, 6.
Bradley, K.A., et al. 2001. Nature 414, 225.
Brossier, F., and Mock, M. 2001. Toxicon 39, 1747.